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Information on EC 4.2.1.105 - 2-hydroxyisoflavanone dehydratase and Organism(s) Glycine max and UniProt Accession Q5NUF3

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.105 2-hydroxyisoflavanone dehydratase
IUBMB Comments
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
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This record set is specific for:
Glycine max
UNIPROT: Q5NUF3
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The taxonomic range for the selected organisms is: Glycine max
The enzyme appears in selected viruses and cellular organisms
Synonyms
2-hydroxyisoflavanone dehydratase, gmhid, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-hydroxyisoflavanone dehydratase
-
GmHID
-
-
HIDH
-
gene name, a gene homologue of 2-hydroxyisoflavanone dehydratase, HIDH2
hydroxyisoflavanone dehydratase
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
2,7,4'-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
CAS REGISTRY NUMBER
COMMENTARY hide
166800-10-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5,7,4'-tetrahydroxyisoflavanone
genistein + H2O
show the reaction diagram
-
-
-
?
2,7,4'-trihydroxyisoflavanone
daidzein + H2O
show the reaction diagram
activity is 40% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
2,7-dihydroxy-4'-methoxyisoflavanone
formononetin + H2O
show the reaction diagram
activity is 15% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
p-nitrophenyl butyrate
?
show the reaction diagram
activity is 0.6% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
additional information
?
-
-
2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-Br-cGMP
expression of the gene of HIDH is dramatically stimulated by 8-Br-cGMP
NO
stimulation of expression
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
2,5,7,4'-tetrahydroxyisoflavanone
pH 7.5, 30°C
0.114
2,7,4'-trihydroxyisoflavanone
pH 7.5, 30°C
0.029
2,7-dihydroxy-4'-methoxyisoflavanone
pH 7.5, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.1
2,5,7,4'-tetrahydroxyisoflavanone
pH 7.5, 30°C
5.3
2,7,4'-trihydroxyisoflavanone
pH 7.5, 30°C
1.6
2,7-dihydroxy-4'-methoxyisoflavanone
pH 7.5, 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
from seedling
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
unifoliate, from seedling
Manually annotated by BRENDA team
-
unifoliate and cotyledonary , from seedling
Manually annotated by BRENDA team
-
exocarp, mesocarp and endocarp
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
additional information
-
quantitative real-time RT-PCR tissue expression analysis, overview
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HIDH_SOYBN
319
0
35138
Swiss-Prot
other Location (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
introduction of soybean HID into the hairy roots of a model legume Lotus japonicus
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akashi, T.; Aoki, T.; Ayabe, S.
Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis
Plant Physiol.
137
882-891
2005
Glycine max (Q5NUF3), Glycine max, Glycyrrhiza echinata (Q5NUF4), Glycyrrhiza echinata
Manually annotated by BRENDA team
Shimamura, M.; Akashi, T.; Sakurai, N.; Suzuki, H.; Saito, K.; Shibata, D.; Ayabe, S.; Aoki, T.
2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
Plant Cell Physiol.
48
1652-1657
2007
Glycine max, Lotus japonicus
Manually annotated by BRENDA team
Suita, K.; Kiryu, T.; Sawada, M.; Mitsui, M.; Nakagawa, M.; Kanamaru, K.; Yamagata, H.
Cyclic GMP acts as a common regulator for the transcriptional activation of the flavonoid biosynthetic pathway in soybean
Planta
229
403-413
2009
Glycine max (Q5NUF3), Glycine max
Manually annotated by BRENDA team
Livingstone, J.M.; Zolotarov, Y.; Stroemvik, M.V.
Transcripts of soybean isoflavone 7-O-glucosyltransferase and hydroxyisoflavanone dehydratase gene homologues are at least as abundant as transcripts of their well known counterparts
Plant Physiol. Biochem.
49
1071-1075
2011
Glycine max, Glycine max Williams 82
Manually annotated by BRENDA team