This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1a) can be considered an equivalent of 'cyanate + H2O = carbamate', where the water molecule is provided by the dehydration of bicarbonate to carbon dioxide , and hence the enzyme is classified as a hydrolase.
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SYSTEMATIC NAME
IUBMB Comments
carbamate hydro-lyase
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1a) can be considered an equivalent of 'cyanate + H2O = carbamate', where the water molecule is provided by the dehydration of bicarbonate to carbon dioxide [2], and hence the enzyme is classified as a hydrolase.
cyanate treatment inhibits germination and early seedling growth of Cyn knockout mutant plants, while wild-type plants show resistance to cyanate stress
one role of cyanases in plants is detoxification, plants containing CYN exhibit resistance to KCNO stress. The conserved residues Ser117 and Glu94 are not only catalytic residues in AtCYN but also contribute to the stability of AtCYN homodecamers. Transcriptional regulation and expression pattern of AtCYN, overview
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Atcyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein