Information on EC 4.2.1.100 - cyclohexa-1,5-dienecarbonyl-CoA hydratase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.2.1.100
-
RECOMMENDED NAME
GeneOntology No.
cyclohexa-1,5-dienecarbonyl-CoA hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
6-hydroxycyclohex-1-enecarbonyl-CoA = cyclohexa-1,5-dienecarbonyl-CoA + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
addition
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Benzoate degradation
-
benzoyl-CoA degradation II (anaerobic)
-
crotonate fermentation (to acetate and cyclohexane carboxylate)
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Metabolic pathways
-
Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
6-hydroxycyclohex-1-enecarbonyl-CoA hydro-lyase (cyclohexa-1,5-dienecarbonyl-CoA-forming)
Forms part of the anaerobic benzoate degradation pathway, which also includes EC 1.3.8.6 [glutaryl-CoA dehydrogenase (ETF)], EC 1.3.7.8 (benzoyl-CoA reductase) and EC 4.2.1.55 (3-hydroyxbutyryl-CoA dehydratase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
cyclohexa-1,5-diene-1-carbonyl-CoA hydratase
-
-
-
-
Cyclohexa-1,5-diene-1-carboxyl-CoA hydratase
-
-
-
-
DCH
o87873
-
dienoyl-CoA hydratase
-
-
-
-
dienoyl-CoA hydratase
o87873
-
EC 4.2.1.102
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-
formerly
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CAS REGISTRY NUMBER
COMMENTARY
214355-79-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
formerly known as Pseudomonas sp. strains K 172 and KB 740
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-
Manually annotated by BRENDA team
strain DSMZ 2059, grown with benzoate as substrate
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-
Manually annotated by BRENDA team
strain DSMZ 7210, grown with benzoate as substrate
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-
Manually annotated by BRENDA team
grown with benzoate as substrate
-
-
Manually annotated by BRENDA team
denitrifying bacterium, inducible enzyme
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyclohex-1-ene-1-carbonyl-CoA + H2O
2-hydroxycyclohexane-1-carbonyl-CoA
show the reaction diagram
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
-
-
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
involved in the benzoyl-CoA pathway
-
-
r
cyclohexa-1,5-dienecarbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
o87873
trans-1,4 water addition, stereochemistry of 6-hydroxycyclohex-1-ene-1-carboxyl-CoA by NMR analysis
-
-
?
cyclohexa-2,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-2-ene-1-carbonyl-CoA
show the reaction diagram
-
only when dithionite is used as an artificial electron donor
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
involved in the benzoyl-CoA pathway
-
-
r
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
6-hydroxycyclohex-1-enecarbonyl-CoA
-
-
0.03
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cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30C
0.085
-
cyclohexa-1,5-diene-1-carbonyl-CoA
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pH 7.5, 30C
60
-
cyclohexa-1,5-diene-1-carbonyl-CoA
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-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
51
-
cyclohexa-1,5-diene-1-carbonyl-CoA
-
-
175
-
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
280
-
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.8
-
-
cell extract, pH 7.5, 30C
2
-
-
cell extract, pH 7.5, 30C
2.5
-
-
cell extract, pH 7.5, 30C
110
-
-
reversible reaction with 6-hydroxycyclohex-1-ene-1-carboxyl-CoA as substrat
350
-
-
purified enzyme, hydration reaction, pH 7.5, 30C
550
-
-
purified enzyme, hydration reaction, pH 7.5, 30C
730
-
-
purified enzyme, dehydration reaction, pH 7.5, 30C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55000
-
-
gel filtration
70000
-
-
Ferguson blot analysis
92000
-
-
gel filtration
115000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
alpha2, 2 * 28000, SDS-PAGE
dimer
-
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis
tetramer
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4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration
trimer
-
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant protein using His-tag
-
DEAE-Sepharose, hydroxyapatite, Mono-Q, Blue-Sepharose
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells
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