Information on EC 4.2.1.100 - cyclohexa-1,5-dienecarbonyl-CoA hydratase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.2.1.100
-
RECOMMENDED NAME
GeneOntology No.
cyclohexa-1,5-dienecarbonyl-CoA hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-hydroxycyclohex-1-ene-1-carbonyl-CoA = cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
-
-
benzoyl-CoA degradation
-
-
benzoyl-CoA degradation II (anaerobic)
-
-
crotonate fermentation (to acetate and cyclohexane carboxylate)
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Metabolic pathways
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Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
6-hydroxycyclohex-1-enecarbonyl-CoA hydro-lyase (cyclohexa-1,5-dienecarbonyl-CoA-forming)
Forms part of the anaerobic benzoate degradation pathway, which also includes EC 1.3.8.6 [glutaryl-CoA dehydrogenase (ETF)], EC 1.3.7.8 (benzoyl-CoA reductase) and EC 4.2.1.55 (3-hydroyxbutyryl-CoA dehydratase).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cyclohexa-1,5-diene-1-carbonyl-CoA hydratase
-
-
-
-
Cyclohexa-1,5-diene-1-carboxyl-CoA hydratase
-
-
-
-
dienoyl-CoA hydratase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
214355-79-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
formerly known as Pseudomonas sp. strains K 172 and KB 740
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-
Manually annotated by BRENDA team
strain DSMZ 2059, grown with benzoate as substrate
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-
Manually annotated by BRENDA team
strain DSMZ 7210, grown with benzoate as substrate
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-
Manually annotated by BRENDA team
grown with benzoate as substrate
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-
Manually annotated by BRENDA team
denitrifying bacterium, inducible enzyme
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
metabolism
-
the enzyme is involved in benzoyl-coenzyme A degradation
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyclohex-1-ene-1-carbonyl-CoA + H2O
2-hydroxycyclohexane-1-carbonyl-CoA
show the reaction diagram
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
-
-
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
involved in the benzoyl-CoA pathway
-
-
r
cyclohexa-1,5-dienecarbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
o87873
trans-1,4 water addition, stereochemistry of 6-hydroxycyclohex-1-ene-1-carboxyl-CoA by NMR analysis
-
-
?
cyclohexa-2,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-2-ene-1-carbonyl-CoA
show the reaction diagram
-
only when dithionite is used as an artificial electron donor
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
-
-
-
?
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-enecarbonyl-CoA
show the reaction diagram
-
involved in the benzoyl-CoA pathway
-
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
6-hydroxycyclohex-1-ene-1-carbonyl-CoA
show the reaction diagram
D3RXI0
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
6-hydroxycyclohex-1-enecarbonyl-CoA
-
-
0.03
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
0.085
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
60
cyclohexa-1,5-diene-1-carbonyl-CoA
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-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
51
cyclohexa-1,5-diene-1-carbonyl-CoA
-
-
175
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
280
cyclohexa-1,5-diene-1-carbonyl-CoA
-
pH 7.5, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.8
-
cell extract, pH 7.5, 30C
2
-
cell extract, pH 7.5, 30C
2.5
-
cell extract, pH 7.5, 30C
31.7
D3RXI0
85C, pH 7.0, highly enriched enzyme
110
-
reversible reaction with 6-hydroxycyclohex-1-ene-1-carboxyl-CoA as substrat
350
-
purified enzyme, hydration reaction, pH 7.5, 30C
550
-
purified enzyme, hydration reaction, pH 7.5, 30C
730
-
purified enzyme, dehydration reaction, pH 7.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4 - 7.6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
85
D3RXI0
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55000
-
gel filtration
285201
70000
-
Ferguson blot analysis
680480
92000
-
gel filtration
680480
115000
-
gel filtration
680480
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
alpha2, 2 * 28000, SDS-PAGE
dimer
-
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis
tetramer
-
4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration
trimer
-
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
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DEAE-Sepharose, hydroxyapatite, Mono-Q, Blue-Sepharose
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
D3RXI0
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells
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