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EC Tree
IUBMB Comments The enzyme catalyses the reversible hydration of gaseous CO2 to carbonic acid, which dissociates to give hydrogencarbonate above neutral pH. It is widespread and found in archaea, bacteria, and eukaryotes. Three distinct classes exist, and appear to have evolved independently. Contains zinc.
The taxonomic range for the selected organisms is: Neisseria gonorrhoeae The enzyme appears in selected viruses and cellular organisms
Synonyms
carbonic anhydrase, ca ix, ca ii, hca ii, hca i, carbonic anhydrase ix, carbonic anhydrase ii, hca ix, ca iv, anhydrase,
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carbonate anhydrase
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Carbonate dehydratase
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Carbonate dehydratase IX
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Carbonate dehydratase VA
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Carbonate dehydratase VB
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Carbonate dehydratase VI
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Carbonate dehydratase VII
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Carbonate dehydratase XII
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Carbonate dehydratase XIV
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carbonic acid anhydrase
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carbonic dehydratase
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dehydratase, carbonate
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Membrane antigen MN
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RCC-associated antigen G250
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Renal cell carcinoma-associated antigen G250
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Salivary carbonic anhydrase
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Secreted carbonic anhydrase
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Tumor antigen HOM-RCC-3.1.3
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CA
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carbonic anhydrase
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-, -, -, -, -, -, -, -, -
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carbonic acid hydro-lyase (carbon-dioxide-forming)
The enzyme catalyses the reversible hydration of gaseous CO2 to carbonic acid, which dissociates to give hydrogencarbonate above neutral pH. It is widespread and found in archaea, bacteria, and eukaryotes. Three distinct classes exist, and appear to have evolved independently. Contains zinc.
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4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
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additional information
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esterase activity with p-nitrophenyl acetate
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CO2 + H2O
H2CO3
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?
CO2 + H2O
H2CO3
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r
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Co2+
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can substitute for Zn2+
Zn2+
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zinc metalloenzyme
Zn2+
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zinc-containing enzyme
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6-ethoxy-2-benzo-thiazolesulfonamide
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CN-
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anion inhibition of the esterase activity
guanidine hydrochloride
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N3-
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anion inhibition of the esterase activity
NCO-
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anion inhibition of the esterase activity
SCN-
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anion inhibition of the esterase activity
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3133
CO2
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purified recombinant enzyme
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0.000025 - 0.000084
6-ethoxy-2-benzo-thiazolesulfonamide
0.027
NCO-
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pH 6.7, 25°C
0.000025
6-ethoxy-2-benzo-thiazolesulfonamide
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pH 7.0, 10°C, mutant H66A
0.000084
6-ethoxy-2-benzo-thiazolesulfonamide
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pH 7.0, 10°C, wild-type enzyme
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Uniprot
brenda
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CAH_NEIGO
252
0
28085
Swiss-Prot
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28000
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x * 28000, SDS-PAGE
28085
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x * 28085, a 26-residue signal peptide is cleaved off by the Escherichia coli processing machinery, thus the recombinant enzyme has a MW of 26314 Da, calculation from nucleotide sequence
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x * 28000, SDS-PAGE
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x * 28085, a 26-residue signal peptide is cleaved off by the Escherichia coli processing machinery, thus the recombinant enzyme has a MW of 26314 Da, calculation from nucleotide sequence
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proteolytic modification
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a 26-residue signal peptide is cleaved off by the E. coli processing machinery
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complex with the inhibitor acetazolamide
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H66A
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higher sensitivity to inhibitor 6-ethoxy-2-benzo-thiazolesulfonamide
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guanidine hydrochloride causes denaturation and inactivation
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Ni-NTA column chromatography
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cloned and expressed in Escherichia coli BL21(DE3)
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expressed in Escherichia coli BL21(DE3) cells
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overexpression in Escherichia coli
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Chirica, L.C.; Elleby, B.; Jonsson, B.H.; Lindskog, S.
The complete sequence, expression in Escherichia coli, purification and some properties of carbonic anhydrase from Neisseria gonorrhoeae
Eur. J. Biochem.
244
755-760
1997
Neisseria gonorrhoeae
brenda
Huang, S.; Xue, Y.; Sauer-Eriksson, E.; Chirica, L.; Lindskog, S.; Jonsson, B.H.
Crystal structure of carbonic anhydrase from Neisseria gonorrhoeae and its complex with the inhibitor acetazolamide
J. Mol. Biol.
283
301-310
1998
Neisseria gonorrhoeae (Q50940), Neisseria gonorrhoeae
brenda
Elleby, B.; Chirica, L.C.; Tu, C.; Zeppezauer, M.; Lindskog, S.
Characterization of carbonic anhydrase from Neisseria gonorrhoeae
Eur. J. Biochem.
268
1613-1619
2001
Neisseria gonorrhoeae
brenda
Liu, Z.; Bartlow, P.; Dilmore, R.M.; Soong, Y.; Pan, Z.; Koepsel, R.; Ataai, M.
Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum
Biotechnol. Prog.
25
68-74
2009
Neisseria gonorrhoeae
brenda