Information on EC 4.1.99.19 - 2-iminoacetate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.99.19
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RECOMMENDED NAME
GeneOntology No.
2-iminoacetate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-tyrosine + S-adenosyl-L-methionine + NADPH = 2-iminoacetate + 4-methylphenol + 5'-deoxyadenosine + L-methionine + NADP+ + H+
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Thiamine metabolism
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thiazole biosynthesis I (facultative anaerobic bacteria)
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vitamin B1 metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-tyrosine 4-methylphenol-lyase (2-iminoacetate-forming)
Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The first stage of catalysis is reduction of the S-adenosyl-L-methionine to produce methionine and a 5-deoxyadenosin-5-yl radical that is crucial for the conversion of the substrate. The reductant is assumed to be NADPH, which is provided by a flavoprotein:NADPH oxidoreductase system [4]. Part of the pathway for thiamine biosynthesis.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-tyrosine + S-adenosyl-L-methionine + reduced acceptor
2-iminoacetate + 4-methylphenol + 5'-deoxyadenosine + L-methionine + acceptor + 2 H+
show the reaction diagram
the reductive cleavage of S-adenosyl-L-methionine yields the highly reactive 5'-deoxyadenosyl radical, which can abstract the phenolic hydrogen atom from tyrosine
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-sulfur centre
contains an oxygen labile Fe-S cluster
[4Fe-4S]-center
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essential for activity
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4Fe-4S cluster
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4Fe-4S-center
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Iron
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iron content is 5.2 mol eq of iron per mol of ThiH, contains an 4F-4S cluster essential for activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44143
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x * 44143, calculated from sequence; x * 44143, electrospray mass spectrometry
additional information
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the 440000 Da complex contains both ThiG and ThiH-His
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 44143, calculated from sequence; x * 44143, electrospray mass spectrometry
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
when isolated under anaerobic conditions, ThiG and ThiH-His co-purify as a large multimeric non-covalent complex
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C-terminally hexahistidine-tagged ThiH(ThiH-His) is expressed in Escherichia coli as a soluble protein from thiGHHis-tag and thiFSGH-His-tag-bearing plasmids
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A26G
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
A272V
the thiH mutant allele results in a thiH null phenotype
A373P
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
A50T
the thiH mutant allele results in a thiH null phenotype
A51V
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
A61A
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
A61T
the thiH mutant allele results in a thiH null phenotype
C285A
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
C285H
the thiH mutant allele results in a thiH null phenotype
C89A
the thiH mutant allele results in a thiH null phenotype
D41V
the thiH mutant allele results in a thiH null phenotype
D6E
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
E131A
the thiH mutant allele results in a thiH null phenotype
E131K
the thiH mutant allele results in a thiH null phenotype
E187D
the thiH mutant allele results in a thiH null phenotype
E29K
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
E55G
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
E55K
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
G130A
the thiH mutant allele results in a thiH null phenotype
G216C
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
G22V
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
G327C
the thiH mutant allele results in a thiH null phenotype
h124Y
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
N87A
the thiH mutant allele results in a thiH null phenotype
N87K
the thiH mutant allele results in a thiH null phenotype
P334Q
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
P347S
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
Q338L
the thiH mutant allele results in a thiH null phenotype
R102P
the thiH mutant allele results in a thiH null phenotype
R205W
the thiH mutant allele results in a thiH null phenotype
T266N
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
V257A
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
V360I
the thiH mutant allele results in a phenotype with thiamine auxotrophy that is corrected by either L-tyrosine or thiazole
W363C
the thiH mutant allele results in a thiH null phenotype
Y190C
the thiH mutant allele results in a thiH null phenotype
Y91A
the thiH mutant allele results in a thiH null phenotype
Show AA Sequence (494 entries)
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