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Information on EC 4.1.99.16 - geosmin synthase and Organism(s) Streptomyces avermitilis and UniProt Accession Q82L49

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.16 geosmin synthase
IUBMB Comments
Requires Mg2+. Geosmin is the cause of the characteristic smell of moist soil. It is a bifunctional enzyme. The N-terminal part of the enzyme is EC 4.2.3.22, germacradienol synthase, and forms germacradienol from FPP. The C-terminal part of the enzyme catalyses the conversion of germacradienol to geosmin via (1S,4aS,8aS)-1,4a-dimethyl-1,2,3,4,4a,5,6,8a-octahydronaphthalene.
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This record set is specific for:
Streptomyces avermitilis
UNIPROT: Q82L49
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Word Map
  • 4.1.99.16
  • odor
  • geosmin-producing
  • taste
  • anabaena
  • cyanobacterial
  • isoprenoid
  • sesquiterpene
  • coelicolor
  • off-flavors
  • bloom
  • freshwater
  • amplicon
  • circinalis
  • 2-methylisoborneol
  • terpenoid
  • terpene
  • trouble
  • dolichospermum
  • on-site
  • aquaculture
  • farnesyl
  • bornet
The taxonomic range for the selected organisms is: Streptomyces avermitilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
geosmin synthase, sce1440, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
germacradienol geosmin-lyase (acetone forming)
Requires Mg2+. Geosmin is the cause of the characteristic smell of moist soil. It is a bifunctional enzyme. The N-terminal part of the enzyme is EC 4.2.3.22, germacradienol synthase, and forms germacradienol from FPP. The C-terminal part of the enzyme catalyses the conversion of germacradienol to geosmin via (1S,4aS,8aS)-1,4a-dimethyl-1,2,3,4,4a,5,6,8a-octahydronaphthalene.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ?
show the reaction diagram
overall reaction of germacradienol/geosmin synthase
presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin
-
?
additional information
?
-
nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
10-20% of the activity with Mg2+
Cu2+
10-20% of the activity with Mg2+
Fe2+
50% of the activity with Mg2+
Mg2+
preferred divalent cation, maximum activity at 10 mM
Mn2+
10-20% of the activity with Mg2+
Zn2+
50% of the activity with Mg2+
additional information
absolute requirement for a divalent cation. No activity with Ni2+ or Ca2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000075
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0031
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
germacradienol/geosmin synthase
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
mutants with a deleted geoA are unable to produce either germacradienol or geosmin
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cane, D.E.; He, X.; Kobayashi, S.; Omura, S.; Ikeda, H.
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase
J. Antibiot.
59
471-479
2006
Streptomyces avermitilis (Q82L49)
Manually annotated by BRENDA team