Information on EC 4.1.3.45 - 3-hydroxybenzoate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.3.45
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RECOMMENDED NAME
GeneOntology No.
3-hydroxybenzoate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chorismate = 3-hydroxybenzoate + pyruvate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phenylalanine, tyrosine and tryptophan biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
chorismate pyruvate-lyase (3-hydroxybenzoate-forming)
The enzyme, found in several bacterial species is involved in biosynthesis of secondary products. The enzyme from the bacterium Xanthomonas campestris pv. campestris also has the activity of EC 4.1.3.40, chorismate lyase [3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzoate + pyruvate + H2O
chorismate
show the reaction diagram
chorismate
3-hydroxybenzoate + pyruvate
show the reaction diagram
chorismate
3-hydroxybenzoate + pyruvate + H2O
show the reaction diagram
additional information
?
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no activity with 3-hydroxybenzoate and 4-hydroxybenzoate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzoate + pyruvate + H2O
chorismate
show the reaction diagram
chorismate
3-hydroxybenzoate + pyruvate
show the reaction diagram
chorismate
3-hydroxybenzoate + pyruvate + H2O
show the reaction diagram
additional information
?
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no activity with 3-hydroxybenzoate and 4-hydroxybenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0261 - 0.53
chorismate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
3-hydroxybenzoate
Streptomyces sp.
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at pH 6.5 and 26C
22.4
chorismate
Streptomyces hygroscopicus
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in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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at 26C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 28
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the enzyme exhibits more than 75% of its maximum activity between 20 and 28C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli and in Streptomyces hygroscopicus BIOT-4010 cells
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