This enzyme catalyses the first step in the biosynthesis of ubiquinone in Escherichia coli and other Gram-negative bacteria . The yeast Saccharomyces cerevisiae can synthesize ubiquinone from either chorismate or tyrosine .
the enzyme is required for initiation of cyanobacterial plastoquinone biosynthesis, together with two 4-hydroxy-3-solanesylbenzoate decarboxylases. The chorismate pyruvate-lyase Sll1797 catalyzes the conversion of chorismate to 4-hydroxybenzoate that is prenylated by Slr0926. Slr1099 and Sll0936 are both involved in the decarboxylation of 4-hydroxy-3-solanesylbenzoate
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1.0 A crystal structure of the enzyme-product complex, 2.0 A structure of the G90A mutant enzyme with bound product, 2.4 A structure of the enzyme complexed with the inhibitor vanillate, 1.9 A structure of the G90A mutant enzyme with the inhibitor vanillate
structure of wild-type enzyme, mutant enzyme C14S and C14S/C81S in enzyme-product complex. Structure is determined by heavy atom methods using the single mutant in its orthorhombic crystal form, and subsequently solved by molecular replacement in both the wild-type and double-mutant forms
expression in Nicotiana tabacum under control of the constitutive plant promoter. The gene product is targeted into the plastid by fusing it to the sequence for the chloroplast transit peptide of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Transgenic plants show high chorismate pyruvate-lyase activity and accumulate 4-hydroxybenzoate as beta-glucosides, with the glucose attached to either the hydroxy or the carboxyl function of 4-hydroxybenzoate. The total content of 4-hydroxybenzoate glucosides is approximately 0.52% of dry weight, which exceeds the content of untransformed plants by at least a factor of 1000
gene sll1797, phylogenetic analysis and tree, expression in Escherichia coli strain BL21AI as GST-tagged protein, and in Escherichia coli strain BW25113 for quantitative expression analysis by real time quantitative PCR. Complementation of ubiquinone-deficient Escherichia coli mutants by catalytic activity of heterologous Sll1797
the ubiC gene is integrated into the chloroplast genome of Nicotiana tabacum under the control of the light-regulated psbA 5'-untranslated region. the limitation for 4-hydroxybenzoate production in nuclear-transformed plants is the activity of chorismate pyruvate-lyase activity. The process becomes substrate-limited only when the enzyme is present at very high levels in the compartment of interest