Information on EC 4.1.3.36 - 1,4-dihydroxy-2-naphthoyl-CoA synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.1.3.36
-
RECOMMENDED NAME
GeneOntology No.
1,4-dihydroxy-2-naphthoyl-CoA synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Claisen condensation
cleavage of 3-hydroxy acid
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1,4-dihydroxy-2-naphthoate biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Metabolic pathways
-
-
Ubiquinone and other terpenoid-quinone biosynthesis
-
-
vitamin K metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA dehydratase (cyclizing)
This enzyme is involved in the synthesis of 1,4-dihydroxy-2-naphthoate, a branch point metabolite leading to the biosynthesis of menaquinone (vitamin K2, in bacteria), phylloquinone (vitamin K1 in plants), and many plant pigments. The coenzyme A group is subsequently removed from the product by EC 3.1.2.28, 1,4-dihydroxy-2-naphthoyl-CoA hydrolase.
CAS REGISTRY NUMBER
COMMENTARY hide
72506-71-9
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-(3-carboxypropyl)-benzoyl-CoA
?
show the reaction diagram
-
-
-
-
?
2-succinylbenzoyl-CoA
1,4-dihydroxy-2-naphthoyl-CoA + H2O
show the reaction diagram
-
-
-
-
?
o-succinylbenzoate
1,4-dihydroxy-2-naphthoyl-CoA + H2O
show the reaction diagram
O-succinylbenzoyl-CoA
1,4-dihydroxy-2-naphthoate + CoA
show the reaction diagram
o-succinylbenzoyl-CoA
1,4-dihydroxy-2-naphthoyl-CoA + H2O
show the reaction diagram
-
-
-
-
?
o-succinylbenzoyl-CoA
1,4-dihydroxy-2-naphthoyl-CoA + H2O + H2O
show the reaction diagram
-
-
-
-
?
O-succinylbenzoyl-CoA
?
show the reaction diagram
o-succinylbenzoyl-CoA
CoA + 1,4-dihydroxy-2-naphthoate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
o-succinylbenzoate
1,4-dihydroxy-2-naphthoyl-CoA + H2O
show the reaction diagram
O-succinylbenzoyl-CoA
1,4-dihydroxy-2-naphthoate + CoA
show the reaction diagram
-
-
-
-
?
O-succinylbenzoyl-CoA
?
show the reaction diagram
o-succinylbenzoyl-CoA
CoA + 1,4-dihydroxy-2-naphthoate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
bicarbonate
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3Z)-3-(3,3-dimethyl-2-oxobutylidene)-3,4-dihydro-2H-1,4-benzoxazin-2-one
-
68.4% inhibition at 0.125 mg/ml, 0.00015 mM o-succinylbenzoate , pH not specified in the publication, temperature not specified in the publication
(3Z)-3-(3,3-dimethyl-2-oxobutylidene)-6-(ethylsulfonyl)-3,4-dihydro-2H-1,4-benzoxazin-2-one
-
60.3% inhibition at 0.125 mg/ml, 0.00015 mM o-succinylbenzoate, pH not specified in the publication, temperature not specified in the publication
(3Z)-6-chloro-3-(4-methyl-2-oxopentylidene)-3,4-dihydro-2H-1,4-benzoxazin-2-one
-
98% inhibition at 0.125 mg/ml, 0.00015 mM o-succinylbenzoate, pH not specified in the publication, temperature not specified in the publication
(3Z)-6-methyl-3-(4-methyl-2-oxopentylidene)-3,4-dihydro-2H-1,4-benzoxazin-2-one
-
77.3% inhibition at 0.125 mg/ml, 0.00015 mM o-succinylbenzoate, pH not specified in the publication, temperature not specified in the publication
1-hydroxy-2-naphthoyl-CoA
2,3-dihydroxybenzoyl-CoA
-
-
2,4-dihydroxybenzoyl-CoA
-
-
2-CoA-4-(2,4-dichlorophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-bromophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-chlorophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-fluorophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-iodophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-methoxyphenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(2-nitrophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(3-chlorophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(4-dichlorophenyl)-4-oxobutanoic acid
-
-
2-CoA-4-(4-methoxyphenyl)-4-oxobutanoic acid
-
-
3-(2,4-dichlorophenyl)-3-oxopropyl-CoA
-
weak inhibition
4-(2,4-dichlorophenyl)-4-oxobutanoyl-CoA
-
-
4-(2-chlorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
-
half-life at 25C, pH 7.4, is 0.4 h
4-(2-methoxyphenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
-
half-life at 25C, pH 7.4, is 11.6 h
4-(4-chlorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
-
half-life at 25C, pH 7.4, is 6.8 h
4-(4-fluorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
-
half-life at 25C, pH 7.4, is 12.2 h
4-oxo-2-[[(1S)-1-phenylethyl]amino]-4-[2-(trifluoromethyl)phenyl]butanoic acid
-
half-life at 25C, pH 7.4, is 0.2 h
4-oxo-4-chlorophenylbutenoyl methyl ester
-
penetrates the cell where it is hydrolyzed and reacts with CoA to generate the active antibacterial compound, mechanism, overview
-
4-oxo-4-phenyl-2-[[(1S)-1-phenylethyl]amino]butanoic acid
-
half-life at 25C, pH 7.4, is 11.6 h
methyl (2Z)-(2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(5-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(6-chloro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(6-fluoro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(6-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(6-nitro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(7-chloro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(7-fluoro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(7-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-(7-nitro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
-
methyl (2Z)-[6-(ethylsulfonyl)-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene]ethanoate
-
-
salicyloyl-CoA
salicylyl-CoA
-
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.106
2-(3-carboxypropyl)-benzoyl-CoA
-
wild type protein protein, pH 7.0, 25C
0.0031 - 0.0402
2-succinylbenzoyl-CoA
0.0028 - 0.0433
o-succinylbenzoate
0.0259
o-succinylbenzoyl-CoA
-
pH 7.0, 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
2-(3-carboxypropyl)-benzoyl-CoA
Mycobacterium tuberculosis
-
wild type protein protein, pH 7.0, 25C
0.00022 - 0.462
2-succinylbenzoyl-CoA
0.0027 - 0.038
o-succinylbenzoate
0.062
o-succinylbenzoyl-CoA
Escherichia coli
-
pH 7.0, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024
2-(3-carboxypropyl)-benzoyl-CoA
Mycobacterium tuberculosis
-
wild type protein protein, pH 7.0, 25C
41715
0.04 - 21
2-succinylbenzoyl-CoA
0.16 - 7.4
o-succinylbenzoate
2.4
o-succinylbenzoyl-CoA
Escherichia coli
-
pH 7.0, 25C
6009
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013
1-hydroxy-2-naphthoyl-CoA
-
pH 7,0, 23C
0.0031
2,3-dihydroxybenzoyl-CoA
-
pH 7,0, 23C
0.0022
2,4-dihydroxybenzoyl-CoA
-
pH 7,0, 23C
0.000049
2-CoA-4-(2,4-dichlorophenyl)-4-oxobutanoic acid
-
pH 7.4, 25C
0.000097
2-CoA-4-(2-chlorophenyl)-4-oxobutanoic acid
-
pH 7.4, 25C
0.00035
2-CoA-4-(4-dichlorophenyl)-4-oxobutanoic acid
-
pH 7.4, 25C
0.0091
methyl (2Z)-(2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
non-competitive inhibitor, pH not specified in the publication, temperature not specified in the publication
0.0225
methyl (2Z)-(6-chloro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
non-competitive inhibitor, pH not specified in the publication, temperature not specified in the publication
0.0115
methyl (2Z)-(6-fluoro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
-
non-competitive inhibitor , pH not specified in the publication, temperature not specified in the publication
0.006
salicylyl-CoA
-
pH 7,0, 23C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000106
2-CoA-4-(2,4-dichlorophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.000135
2-CoA-4-(2-bromophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.000103
2-CoA-4-(2-chlorophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.000204
2-CoA-4-(2-fluorophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.000421
2-CoA-4-(2-iodophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0121
2-CoA-4-(2-methoxyphenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.000154
2-CoA-4-(2-nitrophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0141
2-CoA-4-(3-chlorophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.00047
2-CoA-4-(4-dichlorophenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0335
2-CoA-4-(4-methoxyphenyl)-4-oxobutanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.4
3-(2,4-dichlorophenyl)-3-oxopropyl-CoA
Mycobacterium tuberculosis
-
above, pH 7.4, 25C
0.0022
4-(2,4-dichlorophenyl)-4-oxobutanoyl-CoA
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0084
4-(2-chlorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.12
4-(2-methoxyphenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
117
4-(4-chlorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0144
4-(4-fluorophenyl)-4-oxo-2-[[(1S)-1-phenylethyl]amino]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0032
4-oxo-2-[[(1S)-1-phenylethyl]amino]-4-[2-(trifluoromethyl)phenyl]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.0527
4-oxo-4-phenyl-2-[[(1S)-1-phenylethyl]amino]butanoic acid
Mycobacterium tuberculosis
-
pH 7.4, 25C
0.01
methyl (2Z)-(2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
non-competitive inhibitor Ki' = 0.067 mM, pH not specified in the publication, temperature not specified in the publication
0.0241
methyl (2Z)-(5-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0463
methyl (2Z)-(6-chloro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
non-competitive inhibitor Ki' = 0.0185 mM, pH not specified in the publication, temperature not specified in the publication
0.027
methyl (2Z)-(6-fluoro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
non-competitive inhibitor Ki' = 0.0101 mM, pH not specified in the publication, temperature not specified in the publication
0.0231
methyl (2Z)-(6-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0282
methyl (2Z)-(6-nitro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0357
methyl (2Z)-(7-chloro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.03
methyl (2Z)-(7-fluoro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0182
methyl (2Z)-(7-methyl-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0203
methyl (2Z)-(7-nitro-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene)ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.0179
methyl (2Z)-[6-(ethylsulfonyl)-2-oxo-2H-1,4-benzoxazin-3(4H)-ylidene]ethanoate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Geobacillus kaustophilus (strain HTA426)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Staphylococcus aureus (strain COL)
Synechocystis sp. (strain PCC 6803 / Kazusa)
Synechocystis sp. (strain PCC 6803 / Kazusa)
Synechocystis sp. (strain PCC 6803 / Kazusa)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
185000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
homotrimer
-
3 * 30000, gel filtration
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme free or in complex with bicarbonate or nitrate, hanging drop vapor diffusion method, 0.01 ml of protein solution containing 10 mg/mL purified ecMenB, 25 mM Tris, pH 8.0, and 10% glycerol, including 10 mM NaHCO3 or NaNO3 for the complexed enzyme, is mixed with 0.001 ml of reservoir solution containing reservoir solution containing 300 mM NaCl, 100 mM Tris, pH 7.5, 2% Tacsimate, and 20% PEG 335, equilibration against 0.5 ml of reservoir solution, 22C, X-ray diffraction structure determination and analysis at 2.30 A resolution
-
purified recombinant enzyme, hanging drop method, mixing of protein solution containing 10 mg/ml protein in 10 mM NaHCO3, 10 mM 1-hydroxy-2-naphthoyl-CoA, and 25 mM Tris, pH 8.0, with reservoir solution containing 200 mM (NH4)2SO4 and 23% PEG 3,350 in 100 mM Bis-Tris, pH 5.5, in a 1:1 ratio, 1 week, X-ray diffraction structure determination and analysis at 1.84 A resolution
-
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA
-
sitting drop vapour diffusion method with 30% (v/v) pentaerythritol ethocylate, 0.05 M ammonium sulfate, and 0.05 M bis-Tris pH 6.5
-
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA
-
sitting-drop or hanging-drop vapour-diffusion method, structure of native enzyme determined at 2.15 A resolution and structure of the naphthoyl-CoA complex is determined at 2.3 A resolution
-
sitting drop vapour diffusion method with 0.1 M NaHEPES pH 7.5, 1.6 M ammonium sulfate, 0.2 M NaCl
purified recombinant enzyme, hanging drop method, mixing of protein solution containing 10 mg/ml protein in 10 mM NaHCO3, 5 mM 1-hydroxy-2-naphthoyl-CoA, 5 mM salicyloyl-CoA, 20 mM glycine, pH 9.75, 1% glycerol, and 10 mM NaHCO3 or 0.15 M ammonium acetate, 4% tacsimate, 15% PEG 3350, and 100 mM Bis-Tris, pH 6.0, with reservoir solution containing 0.15 M ammonium acetate, 0.3 M ammonium sulfate, 16% PEG 3350, 100 mM Bis-Tris, pH 5.7, and 10 mM proline or 10 mM taurine, in a 1:1 ratio, 1 week, X-ray diffraction structure determination and analysis at 2.0-2.35 A resolution
-
purified recombinant enzyme, hanging drop vapor diffusion method, 0.01 ml of protein solution containing 10 mg/mL purified scMenB, 20 mM glycine, pH 9.75, and 1% glycerol, with or without 10 mM NaHCO3, is mixed with 0.001 ml of reservoir solution containing of 0.15 M ammonium acetate, 0.02 M L-proline, 0.1 M Bis-Tris, pH 6.1, and 45% MPD, equilibration against 0.5 ml of reservoir solution, 22C, X-ray diffraction structure determination and analysis at 2.04 A resolution
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DMSO stabilizes during purification
-
unstable in the presence of ammonium sulfate
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, E. coli K-12, not less than 6 weeks
-
-20C, Mycobacterium phlei, in 3-(N-morpholino)propanesulfonic acid buffer, pH 6.9, 0.2 mM DTT, 20% DMSO, not less than 6 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, ion exchange chromatography (DEAE), gel filtration
-
His-tag affinity chromatography, tag removed
-
HisTrap Chelating HP5 Superdex 200 gel filtration and HiLoad 16/60 Superdex 200 gel filtration
-
HiTrap Chelating HP column chromatography and HiTrap column chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3) too over 95% purity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) too over 95% purity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
-
expression in Escherichia coli BL21(DE3)
-
expression of enzyme in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3). K273A mutant forms inclusion bodies
-
His-tagged version expressed in Escherichia coli BL21(DE3)
-
recombinant expression
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D163A
-
loss of the ligand binding ability
D163E
-
14% 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
D163N
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
F270A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
G156D
-
no activity
K89A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
K91A
-
site-directed mutagenesis, inactive mutant
R267A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R91A
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
S161A
-
expressed in inclusion bodies and unavailable for the binding test
Y258F
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
Y94F
-
wild type level activity
D185E
-
reduced kcat
D185G
-
no activity
D185N
-
reduced kcat
D192N
-
no activity
S190A
-
reduced kcat
Y287F
-
no activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
medicine
-
mutations in the menB gene, the gene encoding naphthoate synthase, cause the small-colony variant phenotype, small-colony variants are associated with persistent infections and may be selectively enriched during antibody therapy
Show AA Sequence (4384 entries)
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