Information on EC 4.1.3.34 - citryl-CoA lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.3.34
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RECOMMENDED NAME
GeneOntology No.
citryl-CoA lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-citryl-CoA = acetyl-CoA + oxaloacetate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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of an oxo-acid, C-C bond cleavage
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Carbon fixation pathways in prokaryotes
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citrate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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NIL
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reductive TCA cycle II
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citric acid cycle
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SYSTEMATIC NAME
IUBMB Comments
(3S)-citryl-CoA oxaloacetate-lyase (acetyl-CoA-forming)
The enzyme is a component of EC 4.1.3.6 {[citrate (pro-3S)-lyase]}and EC 2.3.3.8 [ATP citrate synthase]. Also acts on (3S)-citryl thioacyl-carrier protein.
CAS REGISTRY NUMBER
COMMENTARY hide
131095-35-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain TK-6, IAM12695
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Manually annotated by BRENDA team
strain TK-6, IAM12695
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Manually annotated by BRENDA team
ATCC13882
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Manually annotated by BRENDA team
gene tauO
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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inactivation of TauO in the STQ0606 (DELTAtauO) mutant leads to an apramycin-resistant and kanamycin-sensitive phenotype
metabolism
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citryl-CoA lyase has a key function in the biosynthesis of dialkylmaleic anhydride, which is converted to tautomycetin, a dialkylmaleic anhydride antibiotic, tautomycetin biosynthesis overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-citryl-CoA
acetyl-CoA + oxaloacetate
show the reaction diagram
(3S)-citryl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + oxaloacetate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3S)-citryl-CoA
acetyl-CoA + oxaloacetate
show the reaction diagram
(3S)-citryl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + oxaloacetate
show the reaction diagram
-
enzyme is involved in citrate fermentation
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additional information
?
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the enzyme is involved in the biosynthesis of dialkylmaleic anhydride in Streptomyces griseochromogenes
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.345
acetyl-CoA
0.082
citryl-CoA
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pH 8.0, 70°C
0.0367 - 0.13
oxaloacetate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
570
citryl-CoA
Hydrogenobacter thermophilus
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pH 8.0, 70°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1
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citrate synthase activity as a side reaction
33
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citrate, ATP, CoA and citryl-CoA forming enzyme CCS in the enzyme assay
280
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purified citryl-CoA as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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10% of maximum activity at pH 7.0, 50% of maximum activity at pH 7.5, 90% of maximum activity at pH 9.0, 30% of maximum activity at pH 9.5
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
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alpha6,beta6,gamma6, 6 * 10000, gamma, + 6 * 33000, beta, + 6 * 55000, alpha
11700
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alpha6,beta6,gamma6, 6 * 11700, gamma, + 6 * 32000, beta, + 6 * 56000, alpha
12000
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alpha6,beta6,gamma6, 6 * 12000, gamma, + 6 * 35000, beta, + 6 * 54000, alpha
14000
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alpha6,beta6,gamma6, 6 * 14000, gamma, + 6 * 37000, beta, + 6 * 54000, alpha
30000
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3 * 30000, homotrimer, SDS-PAGE
31352
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x * 31352, determination of amino acid sequence
31600
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alpha6,beta6,gamma6, 6 + 11400, gamma + 6 * 31600, beta, + 6 * 44600, gamma
33000
-
alpha6,beta6,gamma6, 6 * 10000, gamma, + 6 * 33000, beta, + 6 * 55000, alpha
33354
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x * 33354, calculation from nucleotide sequence
35000
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alpha6,beta6,gamma6, 6 * 12000, gamma, + 6 * 35000, beta, + 6 * 54000, alpha
37000
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alpha6,beta6,gamma6, 6 * 14000, gamma, + 6 * 37000, beta, + 6 * 54000, alpha
44600
-
alpha6,beta6,gamma6, 6 + 11400, gamma + 6 * 31600, beta, + 6 * 44600, gamma
55000
-
alpha6,beta6,gamma6, 6 * 10000, gamma, + 6 * 33000, beta, + 6 * 55000, alpha
56000
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alpha6,beta6,gamma6, 6 * 11700, gamma, + 6 * 32000, beta, + 6 * 56000, alpha
85000
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alpha6beta6gamm1, 6 * 32000, acyllyase beta-subunit, + 6 * 54000, acyltransferase alpha-subunit + 1 * 85000, acyl-carrier-protein gamma-subunit, SDS-PAGE
87000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 90
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thermostable with no detectable loss of activity at 80°C for 10 min, retains 83% after 90°C for 10 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native and recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned, sequenced and expressed in Escherichia coli BL21(DE3)
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expression in Escherichia coli
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gene tauO, cloning in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D362E
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conversion of citrate synthase, EC 4.1.3.7, into citryl-CoA lyase, EC 4.1.3.34
additional information
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inactivation of the genes of the tautomycetin biosynthetic gene cluster, tauI, tauO, and tauK, which encode for putative P450 oxidase, citryl-CoA lyase, and esterase enzymes, respectively. The mutant STQ0606 (DELTAtauO) does not produce any detectable amount of tautomycetin intermediates but can convert dialkylmaleic anhydride to tautomycetin. Mutant STQ1211 (DELTAtauK) accumulates dialkylmaleic anhydride, whereas the cofermentation of mutant STQ1211 (DELTAtauK) and mutant STQ0606 (DELTAtauO) restores the production of tautomycetin
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