Information on EC 4.1.3.2 - malate synthase

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The expected taxonomic range for this enzyme is: Sulfolobus acidocaldarius

EC NUMBER
COMMENTARY hide
4.1.3.2
transferred to EC 2.3.3.9
RECOMMENDED NAME
GeneOntology No.
malate synthase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain DSM 639
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + acetyl-CoA + 5,5'-dithiobis-(2-nitrobenzoic acid)
5-mercapto-2-nitrobenzoic acid + ?
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not dependent on Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0056
acetyl-CoA
-
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
0.044
glyoxylate
-
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
-
after 139fold purification, in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88400
-
2 * 88400, SDS-PAGE
103000
-
2 * 103000, gel filtration
206000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 103000, gel filtration; 2 * 88400, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Phenyl Sepharose HP column chromatography, Superdex 200 gel filtration, Red-Sepharose column chromatography, and Uno-Q1 column chromatography
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