Information on EC 4.1.3.14 - L-erythro-3-hydroxyaspartate aldolase

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The expected taxonomic range for this enzyme is: Paracoccus denitrificans

EC NUMBER
COMMENTARY hide
4.1.3.14
-
RECOMMENDED NAME
GeneOntology No.
L-erythro-3-hydroxyaspartate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-erythro-3-hydroxy-aspartate = glycine + glyoxylate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
aldol condensation
-
elimination
-
-
of an oxo-acid, C-C bond cleavage
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycolate and glyoxylate degradation III
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-
SYSTEMATIC NAME
IUBMB Comments
L-erythro-3-hydroxy-aspartate glyoxylate-lyase (glycine-forming)
A pyridoxal-phosphate protein. The enzyme, purified from the bacterium Paracoccus denitrificans NCIMB 8944, is strictly specific for the L-erythro stereoisomer of 3-hydroxyaspartate. Different from EC 4.1.3.41, erythro-3-hydroxy-D-aspartate aldolase. Requires a divalent cation.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-64-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-3-3,4-dihydroxyphenylserine
?
show the reaction diagram
D-3-3,4-methylenedioxyphenylserine
?
show the reaction diagram
D-3-hydroxyaspartate
glycine + glyoxylate
show the reaction diagram
D-3-phenylserine
?
show the reaction diagram
D-threonine
?
show the reaction diagram
DL-Allothreonine
Acetaldehyde + Gly
show the reaction diagram
-
-
-
-
erythro-3-Hydroxy-L-Asp
Gly + glyoxylate
show the reaction diagram
-
no activity: D-Thr, L-Thr, D-Ser, L-Ser, threo-3-hydroxy-DL-Asp or its beta-methyl derivative
-
-
additional information
?
-
-
induced by glycolate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
induced by glycolate
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.8
erythro-3-hydroxy-L-Asp
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
pH 7: optimal stability around
5223
8.5
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rapid loss of activity above
5223
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
pyridoxal phosphate restores activity after inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 10 mM Tris buffer, pH 8.0, rapid inactivation, the activity of the aged extract being partially restored by pyridoxal phosphate
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE