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Information on EC 4.1.3.1 - isocitrate lyase and Organism(s) Dianthus caryophyllus and UniProt Accession Q05957

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.1 isocitrate lyase
IUBMB Comments
The isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate .
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This record set is specific for:
Dianthus caryophyllus
UNIPROT: Q05957
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Word Map
The taxonomic range for the selected organisms is: Dianthus caryophyllus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
icl, isocitrate lyase, citrate lyase, mtbicl, eggce, isocitritase, isocitratase, isocitric lyase, fpicl1, petal death protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
petal death protein
-
ICL
-
-
-
-
isocitrase
-
-
-
-
isocitratase
-
-
-
-
isocitric lyase
-
-
-
-
isocitritase
-
-
-
-
lyase, isocitrate
-
-
-
-
threo-DS-Isocitrate glyoxylate-lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
isocitrate glyoxylate-lyase (succinate-forming)
The isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate [3].
CAS REGISTRY NUMBER
COMMENTARY hide
9045-78-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-2-ethyl malate
acetate + 2-oxobutanoate
show the reaction diagram
-
-
-
?
(2R)-2-methyl malate
pyruvate + acetate
show the reaction diagram
-
-
-
?
(2R)-ethyl-(3S)-methyl malate
2-oxobutanoate + propanoate
show the reaction diagram
-
-
-
?
(2R)-isobutyl-(3S)-methyl malate
4-methyl-2-oxopentanoate + propanoate
show the reaction diagram
-
-
-
?
(2R)-propyl-(3S)-methyl malate
4-methyl-2-oxopentanoate + propanoate
show the reaction diagram
-
-
-
?
(2R,3R:2S,3S)-2-methyl-isocitrate
succinate + pyruvate
show the reaction diagram
-
-
-
?
(2R,3S)-2,3-dimethyl malate
pyruvate + propanoate
show the reaction diagram
-
-
-
?
(2R,3S)-isocitrate
succinate + glyoxylate
show the reaction diagram
-
-
-
?
(2R,3S:2S,3R)-2-methyl-isocitrate
succinate + pyruvate
show the reaction diagram
-
-
-
?
(2S)-2-methyl malate
pyruvate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
(R)-malate
glyoxylate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
(S)-malate
glyoxylate + acetate
show the reaction diagram
kcat below 1x10-5
-
-
?
oxalacetate
oxalate + acetate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
activating oxalacetate hydrolase activity
Mn2+
activating oxalacetate hydrolase activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
(2R)-2-ethyl malate
pH 7.5, 25°C
0.29
(2R)-2-methyl malate
pH 7.5, 25°C
0.45 - 0.53
(2R)-ethyl-(3S)-methyl malate
8
(2R)-isobutyl-(3S)-methyl malate
pH 7.5, 25°C
0.098
(2R)-propyl-(3S)-methyl malate
pH 7.5, 25°C
0.104
(2R,3R:2S,3S)-2-methyl-isocitrate
pH 7.5, 25°C
0.026 - 0.029
(2R,3S)-2,3-dimethyl malate
0.042
(2R,3S)-isocitrate
pH 7.5, 25°C
0.069
(2R,3S:2S,3R)-2-methyl-isocitrate
pH 7.5, 25°C
0.13
oxalacetate
pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
(2R,3S)-2-ethyl-3-methyl malate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PDP_DIACA
318
0
34180
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
133000
gel filtration
35000
4 * 35000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 35000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C144A
mutant with no enzyme activity
D79A
mutant with 1000fold reduced turnover rate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expresssion in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lu, Z.; Feng, X.; Song, L.; Han, Y.; Kim, A.; Herzberg, O.; Woodson, W.R.; Martin, B.M.; Mariano, P.S.; Dunaway-Mariano, D.
Diversity of function in the isocitrate lyase enzyme superfamily: the Dianthus caryophyllus petal death protein cleaves alpha-keto and alpha-hydroxycarboxylic acids
Biochemistry
44
16365-16376
2005
Dianthus caryophyllus (Q05957), Dianthus caryophyllus
Manually annotated by BRENDA team