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3,4-dihydroxycinnamoyl-CoA + H2O
3,4-dihydroxybenzaldehyde + acetyl-CoA
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
vanillin + acetyl-CoA
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
4-coumaroyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
4-feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
i.e. vanillin
ir
4-hydroxy-3-methoxy-phenyl-beta-hydroxypropionyl-CoA + H2O
vanillin + acetylCoA + feruloylCoA
-
-
-
?
4-hydroxy-3-methoxycinnamoyl-CoA + H2O
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
4-hydroxycinnamoyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
4-trans-feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
feruloyl-CoA
4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
-
-
-
?
feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
feruloyl-CoA + H2O
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
feruloyl-CoA + H2O
vanillin + acetyl-CoA
p-coumaroyl-CoA
4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
in transgenic plants the HCHL enzyme acts upon intermediates of the phenylpropanoid pathway
i.e. vanillin
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
3,4-dihydroxycinnamoyl-CoA + H2O
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
-
-
?
3,4-dihydroxycinnamoyl-CoA + H2O
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
vanillin + acetyl-CoA
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxycinnamoyl-CoA
-
?, ir
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxycinnamoyl-CoA
-
?, ir
4-coumaroyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
-
-
-
-
?
4-coumaroyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
-
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxycinnamoyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxycinnamoyl-CoA + H2O
4-hydroxybenzaldehyde + acetyl-CoA
-
-
-
?
4-trans-feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
i.e. vanillin
?
4-trans-feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
i.e. vanillin
?
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
i.e. 3,4-dihydroxycinnamoyl-CoA
i.e. protocatechuic aldehyde
?, ir
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
i.e. 3,4-dihydroxycinnamoyl-CoA
i.e. protocatechuic aldehyde
?, ir
feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
modelling of the natural substrate feruloyl-CoA in the active site of HCHL reveals a catalytic role for Glu143 and a substrate-specific evidence for Tyr239 from the adjacent monomeric structure
catalyzes the hydration of the acyl-CoA thioester of ferulic acid, hydration of the double bond yields 4-hydroxy-3-methoxy-phenyl-beta-hydroxy-propionyl-CoA, subsequent retro-aldol cleavage leads to vanilin
-
?
feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
first step of reaction
-
-
?
feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
first step of reaction
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
overall reaction
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
overall reaction
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
the enzyme is required for p-coumarate degradation
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
the enzyme is required for p-coumarate degradation
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
4-feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
i.e. vanillin
ir
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
feruloyl-CoA
4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
-
-
-
?
feruloyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
-
?
feruloyl-CoA + H2O
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
vanillin + acetyl-CoA
-
-
-
-
?
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxycinnamoyl-CoA
-
ir
4-coumaroyl-CoA
4-hydroxybenzaldehyde + acetyl-CoA
-
i.e. 4-hydroxycinnamoyl-CoA
-
ir
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
-
-
?
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
i.e. 3,4-dihydroxycinnamoyl-CoA
i.e. protocatechuic aldehyde
ir
caffeoyl-CoA
3,4-dihydroxybenzaldehyde + acetyl-CoA
-
i.e. 3,4-dihydroxycinnamoyl-CoA
i.e. protocatechuic aldehyde
ir
feruloyl-CoA + H2O
vanillin + acetyl-CoA
the enzyme is required for p-coumarate degradation
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
the enzyme is required for p-coumarate degradation
-
-
?
feruloyl-CoA + H2O
vanillin + acetyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
trans-feruloyl-CoA + H2O
4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
-
-
?
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expressed in Escherichia coli
-
expressed in Escherichia coli strain BL21 to yield HCHL protein encoded by Pseudomonas fluorescens strain AN103
-
expressed in Escherichia coli Survival 2 T1 cells and in Capsicum frutescens cultivar Hot Lava
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli strain BL21(DE3)
-
functional expression in hairy root cells of Datura stramonium via infection with Agrobacterium rhizogenes, no accumulation of 4-hydroxybenzaldehyde derivatives occurs, but diminished feruloyl-CoA availability, leading to reduced lignin formation, and formation of new glucoconjugates of 4-hydroxybenzaldehyde derivatives, e.g. 4-hydroxybenzyl alcohol-O-beta-D-glucoside
-
gene encoding an enoyl-SCoA hydratase/lyase isolated, cloned and heterologously expressed in Escherichia coli JM109
-
gene ferB, the ferBA genes form an operon, genetic organization of the fer gene cluster, overview. Transcriptional regulation of ferBA controlled by a MarR-type transcriptional regulator, FerC. The ferC gene is located upstream of ferB. The transcription start site of ferB is mapped at 30 nucleotides upstream from the ferB initiation codon. Constitutive expression of the ferC-ferB intergenic region in SME043 cells, FerC regulates the ferBA transcription through the binding to the ferCferB intergenic region
overexpression in Escherichia coli
Phyllostachys nigra cells are transformed to express 4-hydroxycinnamoyl-CoA hydratase/lyase. The PpHCHL-transformed cells accumulate glucose conjugates of 4-hydroxybenzoic acid and vanillic acid, indicating that the PpHCHL products (aldehydes) are further metabolized by inherent enzymes in the Phyllostachys nigra cells
-
transgenic tobacco cv. XHFD 8 plants expressing various levels of bacterial HCHL, lignin deposition reduced, monolignol biosynthesis and vascular integrity impaired, reduction in stem toughness, cell wall esterified and soluble phenolics reduced, wall-bound accretion of vanillin and vanillic acid, intracellular accumulation of novel metabolites such as hydroxybenzoic and vanillic acid derivatives
-
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Narbad, A.; Gasson, M.J.
Metabolism of ferulic acid via vanillin using a novel CoA-dependent pathway in a newly isolated strain of Pseudomonas fluorescens
Microbiology
144
1397-1405
1998
Bacillus subtilis, Corynebacterium glutamicum, Burkholderia cepacia, Delftia acidovorans, Escherichia coli, Pseudomonas fluorescens, Streptomyces griseus
brenda
Pometto, A.L.; Crawford, D.L.
Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus
Appl. Environ. Microbiol.
45
1582-1585
1983
Streptomyces viridosporus, Streptomyces viridosporus T7A / ATCC 39115
brenda
Mitra, A.; Kitamura, Y.; Gasson, M.J.; Narbad, A.; Parr, A.J.; Pyne, J.; Rhodes, M.J.C.; Sewter, C.; Walton, N.J.
4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL)-an enzyme of phenylpropanoid chain cleavage from Pseudomonas
Arch. Biochem. Biophys.
365
10-16
1999
Pseudomonas fluorescens, Pseudomonas fluorescens AN103
brenda
Overhage, J.; Priefert, H.; Steinbuchel, A.
Biochemical and genetic analysis of ferulic acid catabolism in Pseudomonas sp. strain HR199
Appl. Environ. Microbiol.
65
4837-4847
1999
Pseudomonas sp., Pseudomonas sp. HR 199
brenda
Gasson, M.J.; Kitamura, Y.; McLauchlan, W.R.; Narbad, A.; Parr, A.J.; Parsons, E.L.; Payne, J.; Rhodes, M.J.; Walton, N.J.
Metabolism of ferulic acid to vanillin. A bacterial gene of the enoyl-SCoA hydratase/isomerase superfamily encodes an enzyme for the hydration and cleavage of a hydroxycinnamic acid SCoA thioester
J. Biol. Chem.
273
4163-4170
1998
Delftia acidovorans, Pseudomonas fluorescens
brenda
Mitra, A.; Mayer, M.J.; Mellon, F.A.; Michael, A.J.; Narbad, A.; Parr, A.J.; Waldron, K.W.; Walton, N.J.
4-Hydroxycinnamoyl-CoA hydratase/lyase, an enzyme of phenylpropanoid cleavage from Pseudomonas, causes formation of C(6)-C(1) acid and alcohol glucose conjugates when expressed in hairy roots of Datura stramonium L.
Planta
215
79-89
2002
Pseudomonas fluorescens, Pseudomonas fluorescens AN103
brenda
Leonard, P.M.; Marshall, C.M.; Dodson, E.J.; Walton, N.J.; Grogan, G.
Purification, crystallization and preliminary x-ray crystallographic analysis of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL), a crotonase homologue active in phenylpropanoid metabolism
Acta Crystallogr. Sect. D
D60
2343-2345
2004
Pseudomonas fluorescens, Pseudomonas fluorescens AN103
brenda
Leonard, P.M.; Brzozowski, A.M.; Lebedev, A.; Marshall, C.M.; Smith, D.J.; Verma, C.S.; Walton, N.J.; Grogan, G.
The 1.8 A resolution structure of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL) from Pseudomonas fluorescens, an enzyme that catalyses the transformation of feruloyl-coenzyme A to vanillin
Acta Crystallogr. Sect. D
62
1494-1501
2006
Pseudomonas fluorescens
brenda
Merali, Z.; Mayer, M.J.; Parker, M.L.; Michael, A.J.; Smith, A.C.; Waldron, K.W.
Metabolic diversion of the phenylpropanoid pathway causes cell wall and morphological changes in transgenic tobacco stems
Planta
225
1165-1178
2007
Nicotiana tabacum
brenda
Bennett, J.P.; Bertin, L.; Moulton, B.; Fairlamb, I.J.; Brzozowski, A.M.; Walton, N.J.; Grogan, G.
A ternary complex of hydroxycinnamoyl-CoA hydratase-lyase (HCHL) with acetyl-CoA and vanillin gives insights into substrate specificity and mechanism
Biochem. J.
414
281-289
2008
Pseudomonas fluorescens (O69762), Pseudomonas fluorescens AN103 (O69762)
brenda
Botosoa, E.P.; Blumenstein, C.; MacKenzie, D.A.; Silvestre, V.; Remaud, G.S.; Kwiecien, R.A.; Robins, R.J.
Quantitative isotopic 13C nuclear magnetic resonance at natural abundance to probe enzyme reaction mechanisms via site-specific isotope fractionation: the case of the chain-shortening reaction for the bioconversion of ferulic acid to vanillin
Anal. Biochem.
393
182-188
2009
Streptomyces griseus
brenda
Kasai, D.; Kamimura, N.; Tani, K.; Umeda, S.; Abe, T.; Fukuda, M.; Masai, E.
Characterization of FerC, a MarR-type transcriptional regulator, involved in transcriptional regulation of the ferulate catabolic operon in Sphingobium sp. strain SYK-6
FEMS Microbiol. Lett.
332
68-75
2012
Sphingobium sp. (G2IJ08), Sphingobium sp.
brenda
Kiran, K.; Sonbarse, P.P.; Veeresh, L.; Shetty, N.P.; Parvatam, G.
Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development
3 Biotech
8
365
2018
Decalepis hamiltonii
brenda
Chee, M.J.; Lycett, G.W.; Khoo, T.J.; Chin, C.F.
Bioengineering of the plant culture of Capsicum frutescens with vanillin synthase gene for the production of vanillin
Mol. Biotechnol.
59
1-8
2017
Vanilla planifolia
brenda
Yang, H.; Barros-Rios, J.; Kourteva, G.; Rao, X.; Chen, F.; Shen, H.; Liu, C.; Podstolski, A.; Belanger, F.; Havkin-Frenkel, D.; Dixon, R.A.
A re-evaluation of the final step of vanillin biosynthesis in the orchid Vanilla planifolia
Phytochemistry
139
33-46
2017
no activity in Vanilla planifolia
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Hirakawa, H.; Schaefer, A.L.; Greenberg, E.P.; Harwood, C.S.
Anaerobic p-coumarate degradation by Rhodopseudomonas palustris and identification of CouR, a MarR repressor protein that binds p-coumaroyl coenzyme A
J. Bacteriol.
194
1960-1967
2012
Rhodopseudomonas palustris (Q6N8W7), Rhodopseudomonas palustris ATCC BAA-98 (Q6N8W7)
brenda
Yang, W.; Tang, H.; Ni, J.; Wu, Q.; Hua, D.; Tao, F.; Xu, P.
Characterization of two Streptomyces enzymes that convert ferulic acid to vanillin
PLoS One
8
e67339
2013
Streptomyces sp. (S5LPF1), Streptomyces sp.
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Liberato, M.V.; Araujo, J.N.; Sodre, V.; Goncalves, T.A.; Vilela, N.; Moraes, E.C.; Garcia, W.; Squina, F.M.
The structure of a prokaryotic feruloyl-CoA hydratase-lyase from a lignin-degrading consortium with high oligomerization stability under extreme pHs
Biochim. Biophys. Acta
1868
140344
2020
uncultured organism (A0A2P1BT02)
brenda
Kitaoka, N.; Nomura, T.; Ogita, S.; Kato, Y.
Bioproduction of glucose conjugates of 4-hydroxybenzoic and vanillic acids using bamboo cells transformed to express bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase
J. Biosci. Bioeng.
S1389-1723
30926-0
2020
Pseudomonas putida, Pseudomonas putida KT 2440
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