Information on EC 4.1.2.53 - 2-keto-3-deoxy-L-rhamnonate aldolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.1.2.53
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RECOMMENDED NAME
GeneOntology No.
2-keto-3-deoxy-L-rhamnonate aldolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-L-rhamnonate = pyruvate + (S)-lactaldehyde
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
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L-rhamnose degradation II
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Microbial metabolism in diverse environments
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degradation of hexoses
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-L-rhamnonate (R)-lactaldehyde lyase (pyruvate-forming)
Requires Mg2+ for activity. The enzyme can also use 2-oxo-3-deoxy-L-mannonate, 2-oxo-3-deoxy-L-lyxonate and 4-hydroxy-2-ketoheptane-1,7-dioate (HKHD) as substrates [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-L-lyxonate
?
show the reaction diagram
2-dehydro-3-deoxy-L-mannonate
?
show the reaction diagram
2-dehydro-3-deoxy-L-rhamnonate
pyruvate + (L)-lactaldehyde
show the reaction diagram
2-dehydro-3-deoxy-L-rhamnonate
pyruvate + (R)-lactaldehyde
show the reaction diagram
2-dehydro-3-deoxy-L-xylonate
?
show the reaction diagram
4-hydroxy-2-oxoheptane-1,7-dioate
?
show the reaction diagram
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-
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?
4-hydroxy-2-oxohexanoate
?
show the reaction diagram
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-
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?
4-hydroxy-2-oxopentanoate
?
show the reaction diagram
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-L-rhamnonate
pyruvate + (L)-lactaldehyde
show the reaction diagram
2-dehydro-3-deoxy-L-rhamnonate
pyruvate + (R)-lactaldehyde
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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about 240% activity in the presence of Co2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
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complete inhibition in the presence of Ca2+
Co2+
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about 75% residual activity in the presence of Co2+
Cu2+
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complete inhibition in the presence of Cu2+
Fe2+
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complete inhibition in the presence of Fe2+
Mg2+
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about 25% residual activity in the presence of Mg2+
Ni2+
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about 10% residual activity in the presence of Ni2+
Zn2+
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about 80% residual activity in the presence of Zn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
2-dehydro-3-deoxy-L-lyxonate
0.14
2-dehydro-3-deoxy-L-mannonate
0.078 - 1.37
2-dehydro-3-deoxy-L-rhamnonate
0.1 - 0.15
4-hydroxy-2-oxoheptane-1,7-dioate
0.05
4-hydroxy-2-oxohexanoate
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pH 8.0, 25C, 25C, Ni2+-activated
0.1
4-hydroxy-2-oxopentanoate
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pH 8.0, 25C, 25C, Ni2+-activated
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-dehydro-3-deoxy-L-lyxonate
0.3 - 54.7
2-dehydro-3-deoxy-L-rhamnonate
0.54 - 299
4-hydroxy-2-oxoheptane-1,7-dioate
447
4-hydroxy-2-oxohexanoate
Escherichia coli
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pH 8.0, 25C, 25C, Ni2+-activated
396
4-hydroxy-2-oxopentanoate
Escherichia coli
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pH 8.0, 25C, 25C, Ni2+-activated
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.8
2-dehydro-3-deoxy-L-lyxonate
14733
2.1
2-dehydro-3-deoxy-L-mannonate
26828
5.1 - 60.2
2-dehydro-3-deoxy-L-rhamnonate
4609
3.6 - 2700
4-hydroxy-2-oxoheptane-1,7-dioate
10416
9900
4-hydroxy-2-oxohexanoate
Escherichia coli
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pH 8.0, 25C, 25C, Ni2+-activated
2062
3900
4-hydroxy-2-oxopentanoate
Escherichia coli
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pH 8.0, 25C, 25C, Ni2+-activated
1197
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in a L-rhamnose-defective mutant of Escherichia coli
expressed in Escherichia coli DH5alpha cells
expression in Escherichia coli BL21(DE3)
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Show AA Sequence (301 entries)
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