Information on EC 4.1.2.51 - 2-dehydro-3-deoxy-D-gluconate aldolase

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The expected taxonomic range for this enzyme is: Picrophilus torridus

EC NUMBER
COMMENTARY hide
4.1.2.51
-
RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-D-gluconate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-D-gluconate = pyruvate + D-glyceraldehyde
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Entner-Doudoroff pathway II (non-phosphorylative)
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Galactose metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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Pentose phosphate pathway
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Entner Doudoroff pathway
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)
The enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway. In the direction of aldol synthesis the enzyme catalyses the formation of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at a similar ratio. It shows no activity with 2-dehydro-3-deoxy-D-gluconate 6-phosphate. cf. EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in the nonphosphorylative variant of the EntnerDoudoroff pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
show the reaction diagram
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
show the reaction diagram
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
show the reaction diagram
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
show the reaction diagram
Q6KZI8
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3 - 0.4
2-dehydro-3-deoxy-D-gluconate
2.5 - 8
2-dehydro-3-deoxy-D-gluconate 6-phosphate
4.6
D-glyceraldehyde
pH 6.2, 60C
2.7
pyruvate
pH 6.2, 60C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24.2 - 26.7
2-dehydro-3-deoxy-D-gluconate
0.34 - 0.36
2-dehydro-3-deoxy-D-gluconate 6-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
52 - 89
2-dehydro-3-deoxy-D-gluconate
1172
0.04 - 0.14
2-dehydro-3-deoxy-D-gluconate 6-phosphate
1596
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48
pH 6.2, 60C, substrate: 2-dehydro-3-deoxy-D-gluconate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
4 * 32000, SDS-PAGE
120000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
4 * 32000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
half-life: 20 min
90
half-life: 15 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli