Information on EC 4.1.2.5 - L-threonine aldolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
4.1.2.5
-
RECOMMENDED NAME
GeneOntology No.
L-threonine aldolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-threonine = glycine + acetaldehyde
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
glycine biosynthesis IV
-
Glycine, serine and threonine metabolism
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
threonine degradation IV
-
SYSTEMATIC NAME
IUBMB Comments
L-threonine acetaldehyde-lyase (glycine-forming)
A pyridoxal-phosphate protein. This enzyme is specific for L-threonine and can not utilize L-allo-threonine. Different from EC 4.1.2.49, L-allo-threonine aldolase, and EC 4.1.2.48, low-specificity L-threonine aldolase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
L-TA
Pseudomonas sp. NCIMB10558
-
-
-
L-threonine acetaldehyde-lyase
-
-
L-threonine aldolase
Pseudomonas sp. NCIMB10558
-
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Pseudomonas sp. NCIMB10558
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-allo-threonine
?
show the reaction diagram
Escherichia coli, Pseudomonas sp., Pseudomonas sp. NCIMB10558
-
-
-
-
?
L-threonine
glycine + acetaldehyde
show the reaction diagram
-
-
-
-
r
L-threonine
glycine + acetaldehyde
show the reaction diagram
-
-
-
-
r
L-threonine
glycine + acetaldehyde
show the reaction diagram
-
L-TA preferably accepts L-threonine as substrate
-
-
?
L-threonine
glycine + acetaldehyde
show the reaction diagram
-
no activity with L-allo-threonine
-
-
?
L-threonine
glycine + acetaldehyde
show the reaction diagram
-
the enzyme is highly specific for L-threonine, no activity with D-threonine, no activity with DL-allo-threonine
-
-
r
L-threonine
glycine + acetaldehyde
show the reaction diagram
Pseudomonas sp. NCIMB10558
-
L-TA preferably accepts L-threonine as substrate
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no requirement for metal ions
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate
acetaldehyde
-
the enzymic cleavage of L-threonine stops when about 30% of the amino acid is converted to glycine and acetaldehyde. By dialysis of the incubation medium against buffer the full activity of the enzyme is restored, product inhibition
cysteine
-
activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate
glycine
-
the enzymic cleavage of L-threonine stops when about 30% of the amino acid is converted to glycine and acetaldehyde. By dialysis of the incubation medium against buffer the full activity of the enzyme is restored, product inhibition
hydroxylamine
-
1 mM, complete inhibition
p-chloromercuribenzoate
-
0.01 mM, complete inhibition
Semicarbazide
-
1 mM, complete inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.19
-
L-allo-threonine
-
pH and temperature not specified in the publication
14.6
-
L-allo-threonine
-
pH and temperature not specified in the publication
0.42
-
L-threonine
-
pH 7.0, 30°C
10
-
L-threonine
-
pH and temperature not specified in the publication
14.7
-
L-threonine
-
pH and temperature not specified in the publication
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.4
-
acetaldehyde
-
pH and temperature not specified in the publication
0.01
-
glycine
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication
additional information
-
-
specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.6
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
7.8
-
pH 6.0: about 80% of maximal activity, pH 7.8: about 80% of maximal activity
6.5
8
-
pH 6.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0-5°C, activity slowly decreases to 50% of the original activity after 10 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DEAE-cellulose column chromatography
-
DEAE-Toyopearl column chromatography
-