Reference on EC 4.1.2.47 - (S)-hydroxynitrile lyase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xu, L.L.; Singh, B.K.; Conn, E.E.
Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum
Arch. Biochem. Biophys.
263
256-263
1988
Linum usitatissimum
Selmar, D.; Lieberei, R.; Biehl, B.; Conn, E.E.
alpha-Hydroxynitrile lyase in Hevea brasiliensis and its significance for rapid cyanogenesis
Physiol. Plant.
75
97-101
1989
Hevea brasiliensis
-
White, W.L.B.; Ariaz-Garzon, D.I.; McMahon, J.M.; Sayre, R.T.
Cyanogenesis in Cassava. The role of hydroxynitrile lyase in root cyanide production
Plant Physiol.
116
1219-1225
1998
Manihot esculenta (P52705)
Lauble, H.; Decanniere, K.; Wajant, H.; Förster, S.; Effenberger, F.
Crystallization and preliminary X-ray diffraction analysis of hydroxynitrile lyase from cassava (manihot esculenta)
Acta Crystallogr. Sect. D
55
904-906
1999
Manihot esculenta
-
Wagner, U.G.; Hasslacher, M.; Griengl, H.; Schwab, H.; Kratky, C.
Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis
Structure
4
811-822
1996
Hevea brasiliensis (P52704)
Hasslacher, M.; Kratky, C.; Griengl, H.; Schwab, H.; Kohlwein, S.D.
Hydroxynitrile lyase from Hevea brasiliensis: molecular characteriation and mechanism of enzyme catalysis
Proteins Struct. Funct. Genet.
27
438-449
1997
Hevea brasiliensis
Effenberger, F.
Synthesis and reactions of optically active cyanohydrins
Angew. Chem. Int. Ed. Engl.
33
1555-1564
1994
Hevea brasiliensis
-
Foerster, S.; Roos, J.; Effenberger, F.; Wajant, H.; Sprauer, A.
The first recombinent hydroxynitrile lyase in the synthesis of (S)-cyanohydrins
Angew. Chem. Int. Ed. Engl.
35
437-439
1996
Manihot esculenta (P52705)
-
Klempier, N.; Griengl, H.
Alphatic (S)-cyanohydrins by enzyme catalyzed synthesis
Tetrahedron Lett.
34
4769-4772
1993
Hevea brasiliensis (P52704)
-
Hughes, J.; Lakey, J.H.; Hughes, M.A.
Production and characterization of a plant alpha-hydroxynitrile lyase in Escherichia coli
Biotechnol. Bioeng.
53
332-338
1997
BRENDA: Manihot esculenta
Textmining: Escherichia coli, plant
Hasslacher, M.; Schall, M.; Hayn, M.; Bona, R.; Rumbold, K.; Lueckl, J.; Griengl, H.; Kohlwein, S.D.; Schwab, H.
High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts
Protein Expr. Purif.
11
61-71
1997
Hevea brasiliensis (P52704), Hevea brasiliensis
Chueskul, S.; Chulavatnatol, M.
Properties of alpha-hydroxynitrile lyase from the petiole of cassava (Manihot esculenta Crantz)
Arch. Biochem. Biophys.
334
401-405
1996
Manihot esculenta
Wajant, H.; Foerster, S.
Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis
Plant Sci.
115
25-31
1996
Hevea brasiliensis (P52704)
-
Hasslacher, M.; Schall, M.; Hayn, M.; Griengl, H.; Kohlwein, S.D.; Schwab, H.
(S)-Hydroxynitrile lyase from Hevea brasiliensis
Ann. N. Y. Acad. Sci.
799
707-712
1996
Hevea brasiliensis
Wajant, H.; Foerster, S.; Spauer, A.; Effenberger, F.; Pfizenmaier, K.
Enantioselective synthesis of aliphatic (S)-cyanohydrins in organic solvents using hydroxynitrile lyase from Manihot esculenta
Ann. N. Y. Acad. Sci.
799
771-776
1996
Manihot esculenta
Hughes, J.; Carvalho, J.P.de C.; Hughes, M.A.
Purification, characterization, and cloning of alpha-hydroxynitrile lyase from cassava (Manihot esculenta Crantz)
Arch. Biochem. Biophys.
311
496-502
1994
BRENDA: Manihot esculenta (P52705), Manihot esculenta
Textmining: plant, Cotyledon
Lauble, H.; Förster, S.; Miehlich, B.; Wajant, H.; Effenberger, F.
Structure of hydroxynitrile lyase from Manihot esculenta in complex with substrates acetone and chloroacetone: implications for the mechanism of cyanogenesis
Acta Crystallogr. Sect. D
57
194-200
2001
Manihot esculenta (P52705)
Hanefeld, U.; Stranzl, G.; Straathof, A.J.; Heijnen, J.J.; Bergmann, A.; Mittelbach, R.; Glatter, O.; Kratky, C.
Electrospray ionization mass spectrometry, circular dichroism and SAXS studies of the (S)-hydroxynitrile lyase from Hevea brasiliensis
Biochim. Biophys. Acta
1544
133-142
2001
Hevea brasiliensis
Gruber, K.; Gugganig, M.; Wagner, U.G.; Kratky, C.
Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis
Biol. Chem.
380
993-1000
1999
Hevea brasiliensis
Yan, G.; Cheng, S.; Zhao, G.; Wu, S.; Liu, Y.; Sun, W.
A single residual replacement improves the folding and stability of recombinant cassava hydroxynitrile lyase in E. coli
Biotechnol. Lett.
25
1041-1047
2003
Manihot esculenta
Bühler, H.; Effenberger, F.; Förster, S.; Roos, J.; Wajant, H.
Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta
ChemBioChem
4
211-216
2003
Manihot esculenta (P52705), Manihot esculenta
Effenberger, F.
Enzyme-catalyzed preparation and synthetic applications of optically active cyanohydrins
Chimia
53
3-10
1999
Hevea brasiliensis
-
Effenberger, F.; Foerster, S.; Wajant, H.
Hydroxynitrile lyases in stereoselective catalysis
Curr. Opin. Biotechnol.
11
532-539
2000
Linum usitatissimum, Manihot esculenta
Bauer, M.; Griengl, H.; Steiner, W.
Parameters influencing stability and activity of a S-hydroxynitrile lyase from Hevea brasiliensis in two-phase systems
Enzyme Microb. Technol.
24
514-522
1999
Hevea brasiliensis
-
Costes, D.; Wehtje, E.; Adlercreutz, P.
Hydroxynitrile lyase-catalyzed synthesis of cyanohydrins in organic solvents. Parameters influencing activity and enantiospecificity
Enzyme Microb. Technol.
25
384-391
1999
Hevea brasiliensis, Manihot esculenta
-
Geyer, R.; Kartnig, T.; Griengl, H.; Steiner, W.
Influence of flavonoids on the stability of an (S)-hydroxynitrile lyase from Hevea brasiliensis
Food. Technol. Biotechnol.
39
161-167
2001
Hevea brasiliensis
-
Stranzl, G.R.; Gruber, K.; Steinkellner, G.; Zangger, K.; Schwab, H.; Kratky, C.
Observation of a short, strong hydrogen bond in the active site of hydroxynitrile lyase from Hevea brasiliensis explains a large pKa shift of the catalytic base induced by the reaction intermediate
J. Biol. Chem.
279
3699-3707
2004
Hevea brasiliensis (P52704), Hevea brasiliensis
Lauble, H.; Miehlich, B.; Förster, S.; Wajant, H.; Effenberger, F.
Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from manihot esculenta in complex with acetone cyanohydrin
Protein Sci.
10
1015-1022
2001
Manihot esculenta
Lauble, H.; Miehlich, B.; Förster, S.; Kobler, C.; Wajant, H.; Effenberger, F.
Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta
Protein Sci.
11
65-71
2002
Manihot esculenta (P52705), Manihot esculenta
Zuegg, J.; Gruber, K.; Gugganig, M.; Wagner, U.G.; Kratky, C.
Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis
Protein Sci.
8
1990-2000
1999
BRENDA: Hevea brasiliensis
Textmining: Electron
Gruber, K.
Elucidation of the mode of substrate binding to hydroxynitrile lyase from Hevea brasiliensis
Proteins
44
26-31
2001
Hevea brasiliensis (P52704), Hevea brasiliensis
Froehlich, R.F.G.; Zabelinskaja-Mackova, A.A.; Fechter, M.H.; Griengl, H.
Novel access to chiral 1,1'-disubstituted ferrocene derivatives via double stereoselective cyanohydrin synthesis exploiting the hydroxynitrile lyase from Hevea brasiliensis
Tetrahedron
14
355-362
2003
Hevea brasiliensis
-
Bianchi, P.; Roda, G.; Riva, S.; Danieli, B.; Zabelinskaja-Mackova, A.; Griengl, H.
On the selectivity of oxynitrilases towards alpha-oxygenated aldehydes
Tetrahedron
57
2213-2220
2001
Hevea brasiliensis
-
Avi, M.; Fechter, M.J.; Gruber, K.; Belaj, F.; Pöchlauer, P.; Griengl, H.
Hydroxynitrile lyase catalysed synthesis of heterocyclic (R)- and (S)-cyanohydrins
Tetrahedron
60
10411-10418
2004
Hevea brasiliensis
-
Roos, J.; Effenberger, F.
Hydroxynitrile lyase catalyzed enantioselective HCN addition to O-protected alpha-hydroxyaldehydes
Tetrahedron Asymmetry
10
2817-2828
1999
Manihot esculenta
-
Griengl, H.; Schwab, H.; Fechter, M.
The synthesis of chiral cyanohydrins by oxynitrilases
Trends Biotechnol.
18
252-256
2000
Hevea brasiliensis, Manihot esculenta
Geyer, R.; Kartnig, T.; Griengl, H.; Steiner, W.
Influence of flavonoids on the stability of an (S)-hydroxynitrile lyase from Hevea brasiliensis
Food Technol. Biotechnol.
39
161-167
2001
Hevea brasiliensis
-
Xu, R.; Zong, M.H.; Liu, Y.Y.; He, J.; Zhang, Y.Y.; Lou, W.Y.
Enzymatic enantioselective transcyanation of silicon-containing aliphatic ketone with (S)-hydroxynitrile lyase from Manihot esculenta
Appl. Microbiol. Biotechnol.
66
27-33
2004
Manihot esculenta
Lou, W.Y.; Xu, R.; Zong, M.H.
Hydroxynitrile lyase catalysis in ionic liquid-containing systems
Biotechnol. Lett.
27
1387-1390
2005
Manihot esculenta
Bühler, H.; Miehlich, B.; Effenberger, F.
Inversion of stereoselectivity by applying mutants of the hydroxynitrile lyase from Manihot esculenta
ChemBioChem
6
711-717
2005
Manihot esculenta
Gruber, K.; Gartler, G.; Krammer, B.; Schwab, H.; Kratky, C.
Reaction mechanism of hydroxynitrile lyases of the alpha/beta-hydrolase superfamily: The three-dimensional structure of the transient enzyme-substrate complex certifies the crucial role of LYS236
J. Biol. Chem.
279
20501-20510
2004
Hevea brasiliensis (P52704), Hevea brasiliensis
Yeow, Y.L.; Leong, Y.K.; Cheah, M.Y.; Dang, H.D.; Law, C.K.
Cleaving of S-mandelonitrile catalyzed by S-hydroxynitrile lyase from Hevea brasiliensis--a kinetic investigation based on the rate curve method
J. Biotechnol.
111
31-39
2004
BRENDA: Hevea brasiliensis (P52704), Hevea brasiliensis
Textmining: Hevea
Siritunga, D.; Arias-Garzon, D.; White, W.; Sayre, R.T.
Over-expression of hydroxynitrile lyase in transgenic cassava roots accelerates cyanogenesis and food detoxification
Plant Biotechnol. J.
2
37-43
2004
Manihot esculenta
Gaisberger, R.P.; Fechter, M.H.; Griengl, H.
The first hydroxynitrile lyase catalysed cyanohydrin formation in ionic liquids
Tetrahedron
15
2959-2963
2004
Hevea brasiliensis
-
Veum, L.; Hanefeld, U.; Pierre, A.
The first encapsulation of hydroxynitrile lyase from Hevea brasiliensis in a sol-gel matrix
Tetrahedron
60
10419-10425
2004
Hevea brasiliensis
-
Cabirol, F.L.; Hanefeld, U.; Sheldon, R.A.
Immobilized hydroxynitrile lyases for enantioselective synthesis of cyanohydrins: sol-gels and cross-linked enzyme aggregates
Adv. Synth. Catal.
348
1645-1654
2006
Hevea brasiliensis
-
Purkarthofer, T.; Skranc, W.; Schuster, C.; Griengl, H.
Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry
Appl. Microbiol. Biotechnol.
76
309-320
2007
Hevea brasiliensis
Krammer, B.; Rumbold, K.; Tschemmernegg, M.; Poechlauer, P.; Schwab, H.
A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening
J. Biotechnol.
129
151-161
2007
Hevea brasiliensis (P52704)
Rustler, S.; Motejadded, H.; Altenbuchner, J.; Stolz, A.
Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid
Appl. Microbiol. Biotechnol.
80
87-97
2008
BRENDA: Manihot esculenta
Textmining: Pseudomonas fluorescens, Komagataella pastoris
von Langermann, J.; Guterl, J.K.; Pohl, M.; Wajant, H.; Kragl, U.
Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values
Bioprocess Biosyst. Eng.
31
155-161
2008
Manihot esculenta (P52705), Manihot esculenta
Semba, H.; Dobashi, Y.; Matsui, T.
Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor
Biosci. Biotechnol. Biochem.
72
1457-1463
2008
BRENDA: Manihot esculenta (P52705), Manihot esculenta
Textmining: Saccharomyces cerevisiae, Transformation
Koch, K.; van den Berg, R.J.; Nieuwland, P.J.; Wijtmans, R.; Wubbolts, M.G.; Schoemaker, H.E.; Rutjes, F.P.; van Hest, J.C.
Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate
Chem. Eng. J.
135
S89-S92
2008
Hevea brasiliensis
-
Avi, M.; Wiedner, R.M.; Griengl, H.; Schwab, H.
Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4-oxocyclohexyl)acetonitrile as the model
Chemistry
14
11415-11422
2008
Hevea brasiliensis (P52704)
Schmidt, A.; Gruber, K.; Kratky, C.; Lamzin, V.S.
Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis
J. Biol. Chem.
283
21827-21836
2008
Hevea brasiliensis (P52704)
Ueberbacher, B.J.; Griengl, H.; Weber, H.
Chemo-enzymatic synthesis of new ferrocenyl-oxazolidinones and their application as chiral auxiliaries
Tetrahedron Asymmetry
19
838-846
2008
Hevea brasiliensis (P52704)
-
Wagner, U.G.; Schall, M.; Hasslacher, M.; Hayn, M.; Griengl, H.; Schwab, H.; Kratky, C.
Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis
Acta Crystallogr. Sect. D
52
591-593
1996
Hevea brasiliensis (P52704)
Van Pelt, S.; Van Rantwijk, F.; Sheldon, R.
Synthesis of aliphatic (S)-alpha-hydroxycarboxylic amides using a one-pot bienzymatic cascade of immobilised oxynitrilase and nitrile hydratase
Adv. Synth. Catal.
351
397-404
2009
Manihot esculenta
-
Bauer, M.; Griengl, H.; Steiner, W.
Kinetic studies on the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis using initial rate methods and progress curve analysis
Biotechnol. Bioeng.
62
20-29
1999
BRENDA: Hevea brasiliensis (P52704), Hevea brasiliensis
Textmining: Hevea
Andexer, J.; Guterl, J.K.; Pohl, M.; Eggert, T.
A high-throughput screening assay for hydroxynitrile lyase activity
Chem. Commun. (Camb. )
28
4201-4203
2006
Manihot esculenta (P52705)
Fechter, M.H.; Gruber, K.; Avi, M.; Skranc, W.; Schuster, C.; Pöchlauer, P.; Klepp, K.O.; Griengl, H.
Stereoselective biocatalytic synthesis of (S)-2-hydroxy-2-methylbutyric acid via substrate engineering by using "thio-disguised" precursors and oxynitrilase catalysis
Chemistry
13
3369-3376
2007
Hevea brasiliensis (P52704)
Hickel, A.; Graupner, M.; Lehner, D.; Hemnetter, A.; Glatter, O.; Griengl, H.
Stability of the hydroxynitrile lyase from Hevea brasiliensis: a fluorescence and dynamic light scattering study
Enzyme Microb. Technol.
21
361-366
1997
Hevea brasiliensis (P52704)
-
Wajant, H.; Pfizenmaier, K.
Identification of potential active-site residues in the hydroxynitrile lyase from Manihot esculenta by site-directed mutagenesis
J. Biol. Chem.
271
25830-25834
1996
Manihot esculenta (P52705), Manihot esculenta
Hasslacher, M.; Schall, M.; Hayn, M.; Griengl, H.; Kohlwein, S.D.; Schwab, H.
Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cerevisiae and identification of an active site residue
J. Biol. Chem.
271
5884-5891
1996
BRENDA: Hevea brasiliensis (P52704), Hevea brasiliensis
Textmining: Escherichia, Escherichia coli, Saccharomyces, Saccharomyces cerevisiae, Manihot esculenta, Oryza sativa
Trummler, K.; Wajant, H.
Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases
J. Biol. Chem.
272
4770-4774
1997
Linum usitatissimum, Linum usitatissimum (P93243), Escherichia coli, Prunus serotina
Gartler, G.; Kratky, C.; Gruber, K.
Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis
J. Biotechnol.
129
87-97
2007
Hevea brasiliensis (P52704), Hevea brasiliensis
Guterl, J.K.
Andexer, J.N.; Sehl, T.; von Langermann, J.; Frindi-Wosch, I.; Rosenkranz, T.; Fitter, J.; Gruber, K.; Kragl, U.; Eggert, T.; Pohl, M.: Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold
J. Biotechnol.
141
166-173
2009
Hevea brasiliensis, Manihot esculenta, Manihot esculenta (P52705)
Bauer, M.; Geyer, R.; Boy, M.; Griegl, G.; Steiner, W.
Stability of the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis
J. Mol. Catal. B
5
343-347
1998
Hevea brasiliensis (P52704)
-
Cui, F.C.; Pan, X.L.; Liu, J.Y.
Catalytic mechanism of hydroxynitrile lyase from Hevea brasiliensis: a theoretical investigation
J. Phys. Chem. B
114
9622-9628
2010
Hevea brasiliensis, Hevea brasiliensis (P52704)
Hasslacher, M.; Kratky, C.; Griengl, H.; Schwab, H.; Kohlwein, S.D.
Hydroxynitrile lyase from Hevea brasiliensis: molecular characterization and mechanism of enzyme catalysis
Proteins
27
438-449
1997
Hevea brasiliensis (P52704), Hevea brasiliensis
Fröhlich, R.F.G.; Zabelinskaja-Mackova, A.A.; Fechter, M.H.; Griengl, H.
Novel access to chiral 1,1'-disubstituted ferrocene derivatives via double stereoselective cyanohydrin synthesis exploiting the hydroxynitrile lyase from Hevea brasiliensis
Tetrahedron Asymmetry
14
355-362
2003
Hevea brasiliensis (P52704)
-
Klempier, N.; Pichler, U.; Griengl, H.
Synthesis of alpha,beta-unsaturated (S)-cyanohydrins using the oxynitrilase from Hevea brasiliensis
Tetrahedron Asymmetry
6
845-848
1995
Hevea brasiliensis (P52704)
-
Schmidt, M.; Herve, S.; KLempier, N.; Griengl, H.
Preparation of optically active cyanohydrins using the (S)-hydroxynitrile lyase from Hevea brasiliensis
Tetrahedron
52
7833-7840
1996
Hevea brasiliensis (P52704)
-
Caruso, C.S.; de Fatima Travensolo, R.; de Campus Bicudo, R.; de Macedo Lemos, E.G.; Ulian de Araujo, A.P.; Carrilho, E.
alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization
Microb. Pathog.
47
118-127
2009
Xylella fastidiosa, Arabidopsis thaliana
Sosedov, O.; Baum, S.; Buerger, S.; Matzer, K.; Kiziak, C.; Stolz, A.
Construction and application of variants of the Pseudomonas fluorescens EBC191 arylacetonitrilase for increased production of acids or amides
Appl. Environ. Microbiol.
76
3668-3674
2010
Escherichia coli, plasmids
Yuryev, R.; Purkarthofer, T.; Gruber, M.; Griengl, H.; Liese, A.
Kinetic studies of the asymmetric Henry reaction catalyzed by hydroxynitrile lyase from Hevea brasiliensis
Biocatal. Biotransform.
28
348-356
2010
Hevea brasiliensis
-
Yuryev, R.; Briechle, S.; Gruber-Khadjawi, M.; Griengl, H.; Liese, A.
Asymmetric retro-Henry reaction catalyzed by hydroxynitrile lyase from Hevea brasiliensis
ChemCatChem
2
981-986
2010
Hevea brasiliensis
-
Semba, H.; Ichige, E.; Imanaka, T.; Atomi, H.; Aoyagi, H.
Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture
Methods Mol. Biol.
643
133-144
2010
BRENDA: Manihot esculenta (P52705), Manihot esculenta
Textmining: Escherichia coli
Dadashipour, M.; Fukuta, Y.; Asano, Y.
Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems
Protein Expr. Purif.
77
92-97
2010
BRENDA: Manihot esculenta
Textmining: Escherichia coli
Padhi, S.K.; Fujii, R.; Legatt, G.A.; Fossum, S.L.; Berchtold, R.; Kazlauskas, R.J.
Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions
Chem. Biol.
17
863-871
2010
Hevea brasiliensis, Manihot esculenta
Fuhshuku, K.; Asano, Y.
Synthesis of (R)-beta-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system
J. Biotechnol.
153
153-159
2011
Hevea brasiliensis
Narayanan, N.N.; Ihemere, U.; Ellery, C.; Sayre, R.T.
Overexpression of hydroxynitrile lyase in cassava roots elevates protein and free amino acids while reducing residual cyanogen levels
PLoS ONE
6
e21996
2011
Manihot esculenta
Asano, Y.; Dadashipour, M.; Yamazaki, M.; Doi, N.; Komeda, H.
Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants
Protein Eng. Des. Sel.
24
607-616
2011
Manihot esculenta (P52705)
Baum, S.; Williamson, D.S.; Sewell, T.; Stolz, A.
Conversion of sterically demanding alpha,alpha-disubstituted phenylacetonitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191
Appl. Environ. Microbiol.
78
48-57
2012
Pseudomonas fluorescens (Q5EG61), Pseudomonas fluorescens, Manihot esculenta
Andexer, J.N.; Staunig, N.; Eggert, T.; Kratky, C.; Pohl, M.; Gruber, K.
Hydroxynitrile lyases with alpha/beta-hydrolase fold: two enzymes with almost identical 3D structures but opposite enantioselectivities and different reaction mechanisms
ChemBioChem
13
1932-1939
2012
Hevea brasiliensis
Dadashipour, M.; Yamazaki, M.; Momonoi, K.; Tamura, K.; Fuhshuku, K.; Kanase, Y.; Uchimura, E.; Kaiyun, G.; Asano, Y.
S-selective hydroxynitrile lyase from a plant Baliospermum montanum: molecular characterization of recombinant enzyme
J. Biotechnol.
153
100-110
2011
BRENDA: Baliospermum montanum (D1MX73), Baliospermum montanum
Textmining: plant
Sosedov, O.; Stolz, A.
Improvement of the amides forming capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 by site-directed mutagenesis
Appl. Microbiol. Biotechnol.
99
2623-2635
2015
Escherichia coli, Manihot esculenta, plant
Von Langermann, J.; Wapenhensch, S.
Hydroxynitrile lyase-catalyzed synthesis of enantiopure cyanohydrins in Biocatalytic Active Static Emulsions (BASE) with suppression of the non-enzymatic side reaction
Adv. Synth. Catal.
356
2989-2997
2014
Manihot esculenta (P52705)
-
Nakano, S.; Dadashipour, M.; Asano, Y.
Structural and functional analysis of hydroxynitrile lyase from Baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics
Biochim. Biophys. Acta
1844
2059-2067
2014
BRENDA: Baliospermum montanum (D1MX73), Baliospermum montanum, Manihot esculenta (P52705), Manihot esculenta
Textmining: Hevea brasiliensis
von Langermann, J.; Nedrud, D.M.; Kazlauskas, R.J.
Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters
ChemBioChem
15
1931-1938
2014
Hevea brasiliensis (P52704), Hevea brasiliensis
Kawahara, N.; Asano, Y.
Mutagenesis of an Asn156 residue in a surface region of S-selective hydroxynitrile lyase from Baliospermum montanum enhances catalytic efficiency and enantioselectivity
ChemBioChem
16
1891-1895
2015
Baliospermum montanum (D1MX73), Baliospermum montanum
Asano, Y.; Kawahara, N.
A new S-hydroxynitrile lyase from Baliospermum montanum - its structure, molecular dynamics simulation, and improvement by protein engineering
Ind. Biotechnol.
12
91-97
2016
Baliospermum montanum
-
Van Rantwijk, F.; Stolz, A.
Enzymatic cascade synthesis of (S)-2-hydroxycarboxylic amides and acids Cascade reactions employing a hydroxynitrile lyase, nitrile-converting enzymes and an amidase
J. Mol. Catal. B
114
25-30
2015
Manihot esculenta (P52705)
-
Rauwerdink, A.; Lunzer, M.; Devamani, T.; Jones, B.; Mooney, J.; Zhang, Z.J.; Xu, J.H.; Kazlauskas, R.J.; Dean, A.M.
Evolution of a catalytic mechanism
Mol. Biol. Evol.
33
971-979
2016
Hevea brasiliensis
Zhao, Y.; Chen, N.; Mo, Y.; Cao, Z.
A full picture of enzymatic catalysis by hydroxynitrile lyases from Hevea brasiliensis Protonation dependent reaction steps and residue-gated movement of the substrate and the product
Phys. Chem. Chem. Phys.
16
26864-26875
2014
Hevea brasiliensis (P52704)
Semba, H; Ichige, E; Imanaka, T; Atomi, H; Aoyagi, H
Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture.
Appl Microbiol Biotechnol
79
563-9
2008
Manihot esculenta
Hughes, J; Keresztessy, Z; Brown, K; Suhandono, S; Hughes, MA
Genomic organization and structure of alpha-hydroxynitrile lyase in cassava (Manihot esculenta Crantz).
Arch Biochem Biophys
356
107-16
1998
Manihot esculenta
Hanefeld, U; Straathof, AJ; Heijnen, JJ
Study of the (S)-hydroxynitrile lyase from Hevea brasiliensis: mechanistic implications.
Biochim Biophys Acta
1432
185-93
1999
Hevea brasiliensis
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