Information on EC 4.1.2.33 - fucosterol-epoxide lyase

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The expected taxonomic range for this enzyme is: Manduca sexta

EC NUMBER
COMMENTARY hide
4.1.2.33
-
RECOMMENDED NAME
GeneOntology No.
fucosterol-epoxide lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(24R,241R)-fucosterol epoxide = desmosterol + acetaldehyde
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
reversal of an aldol condensation
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-
-
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SYSTEMATIC NAME
IUBMB Comments
(24R,241R)-fucosterol-epoxide acetaldehyde-lyase (desmosterol-forming)
The insect enzyme is involved in the conversion of sitosterol into cholesterol.
CAS REGISTRY NUMBER
COMMENTARY hide
99676-42-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
tobacco hornworm
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
show the reaction diagram
(24RS,28RS)-fucosterol epoxide
?
show the reaction diagram
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-
-
-
?
(24S,28S)-fucosterol epoxide
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
10 mM: 16% of maximal activity
Chymotrypsin
-
0.05 mg/ml: 57% of maximal activity
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D-glucose
-
200 mM: 42% of maximal activity
EDTA
-
10 mM: 66% of maximal activity
Fe2+
-
10 mM: 18% of maximal activity
Fe3+
-
10 mM: 26% of maximal activity
glutathione
-
oxidized, 10 mM: 58% of maximal activity, reduced: 10 mM, 89% of maximal activity
imidazole
-
10 mM: 99% of maximal activity
K+
-
10 mM: 71% of maximal activity
Lubrol Px
-
1% (w/v): complete inhibition
Mg2+
-
10 mM: 75% of maximal activity
N-ethylmaleimide
-
10 mM: 77% of maximal activity
NAD+
-
10 mM: 95% of maximal activity
phenylmethylsulfonyl fluoride
-
10 mM: 63% of maximal activity
Staphylococcus aureus V8 protease
-
0.05 mg/ml: 20% of maximal activity
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Sucrose
-
200 mM: 73% of maximal activity
Triton X-100
-
1% (w/v): 12% of maximal activity
Zn2+
-
10 mM: 9% of maximal activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
10 mM: 103% of maximal activity, slight activation
NADH
-
10 mM: 139% of maximal activity, activation
NADP+
-
10 mM: 109% of maximal activity, activation
NADPH
-
10 mM: 106% of maximal activity, slight activation
p-hydroxymercuribenzoate
-
1 mM: 102% of maximal activity, slight activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
(24RS,28RS)-fucosterol epoxide
-
37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
broad range of activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no activity is lost in a single freeze-thaw cycle
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, no activity is lost in a single freeze-cycle
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
larval tobacco hornworm, partial
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