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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
phenylserine aldolase,
more
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5-dehydro-2-deoxy-D-gluconate-6-phosphate malonate-semialdehyde-lyase
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aldolase, phenylserine
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L-threo-3-Phenylserine = glycine + benzaldehyde
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elimination
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of an aldehyde, C-C bond cleavage
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L-threo-3-phenylserine benzaldehyde-lyase (glycine-forming)
A pyridoxal-phosphate protein.
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glycine + benzaldehyde
L-threo-3-phenylserine + L-erythro-3-phenylserine
L-allo-Thr
glycine + acetaldehyde
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?
L-erythro-3-phenylserine
glycine + benzaldehyde
no activity with D-erythro-3-phenylserine
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r
L-Thr
glycine + acetaldehyde
L-threo-3-phenylserine
glycine + benzaldehyde
L-threo-phenylserine
glycine + benzaldehyde
additional information
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glycine + benzaldehyde
L-threo-3-phenylserine + L-erythro-3-phenylserine
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glycine + benzaldehyde
L-threo-3-phenylserine + L-erythro-3-phenylserine
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L-Thr
glycine + acetaldehyde
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L-Thr
glycine + acetaldehyde
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L-threo-3-phenylserine
glycine + benzaldehyde
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r
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L-threo-3-phenylserine
glycine + benzaldehyde
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no activity with D-threo-3-phenylserine
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L-threo-3-phenylserine
glycine + benzaldehyde
no activity with D-threo-3-phenylserine
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r
L-threo-3-phenylserine
glycine + benzaldehyde
no activity with D-threo-3-phenylserine
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r
L-threo-phenylserine
glycine + benzaldehyde
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L-threo-phenylserine
glycine + benzaldehyde
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additional information
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enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
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additional information
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enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
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additional information
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inducible enzyme
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additional information
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inducible enzyme
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additional information
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no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine
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additional information
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no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine
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additional information
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inducible enzyme
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additional information
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no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine
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additional information
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additional information
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inducible enzyme
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additional information
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inducible enzyme
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additional information
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inducible enzyme
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pyridoxal 5'-phosphate
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coenzyme
pyridoxal 5'-phosphate
0.7 mol of pyridoxal 5'-phosphate per mol of subunit, K213 of the enzyme probably forms a Schiff base with pyridoxal 5'-phosphate
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3-(ethylamino)phenol
competitive against DL-threo-3-phenylserine
D-cycloserine
1 mM, 98% inhibition
DL-3-hydroxy-n-butyrate
20 mM, 30% inhibition
DL-3-hydroxynorvaline
20 mM, 49% inhibition
DL-3-hydroxyphenylethylamine
20 mM, 79% inhibition
L-3-phenyllactate
10 mM, 39% inhibition
p-chloromercuribenzoate
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phenylhydrazine
1 mM, 52% inhibition
Semicarbazide
1 mM, 97% inhibition
hydroxylamine
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hydroxylamine
1 mM, 97% inhibition
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22
L-allo-Thr
pH 8.5, 30°C
4.6
L-erythro-3-phenylserine
pH 8.5, 30°C
1.3
L-threo-3-phenylserine
pH 8.5, 30°C
0.035 - 2.8
L-threo-phenylserine
0.035
L-threo-phenylserine
wild-type, pH 8.0, 30°C
0.065
L-threo-phenylserine
mutant D95N/E96S, pH 8.0, 30°C
0.071
L-threo-phenylserine
mutant D95G/E96G, pH 8.0, 30°C
0.17
L-threo-phenylserine
mutant D95C, pH 8.0, 30°C
0.19
L-threo-phenylserine
mutant D95L, pH 8.0, 30°C
0.24
L-threo-phenylserine
mutant D176E, pH 8.0, 30°C
0.31
L-threo-phenylserine
mutant D95Y, pH 8.0, 30°C
0.58
L-threo-phenylserine
mutant D95M, pH 8.0, 30°C
0.78
L-threo-phenylserine
mutant D95W, pH 8.0, 30°C
2.8
L-threo-phenylserine
mutant D95Y/E96T, pH 8.0, 30°C
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1300
L-allo-Thr
pH 8.5, 30°C
7900
L-erythro-3-phenylserine
pH 8.5, 30°C
2300
L-threo-3-phenylserine
pH 8.5, 30°C
0.1 - 67.4
L-threo-phenylserine
0.1
L-threo-phenylserine
mutant D176E, pH 8.0, 30°C
0.78
L-threo-phenylserine
mutant D95W, pH 8.0, 30°C
2.8
L-threo-phenylserine
mutant D95Y/E96T, pH 8.0, 30°C
5.5
L-threo-phenylserine
mutant D95N/E96S, pH 8.0, 30°C
6.8
L-threo-phenylserine
mutant D95G/E96G, pH 8.0, 30°C
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L-threo-phenylserine
mutant D95Y, pH 8.0, 30°C
21
L-threo-phenylserine
mutant D95M, pH 8.0, 30°C
22
L-threo-phenylserine
mutant D95C, pH 8.0, 30°C
23
L-threo-phenylserine
mutant D95L, pH 8.0, 30°C
67.4
L-threo-phenylserine
wild-type, pH 8.0, 30°C
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0.42 - 1900
L-threo-phenylserine
0.42
L-threo-phenylserine
mutant D176E, pH 8.0, 30°C
22
L-threo-phenylserine
mutant D95W, pH 8.0, 30°C
36
L-threo-phenylserine
mutant D95M, pH 8.0, 30°C
45
L-threo-phenylserine
mutant D95Y, pH 8.0, 30°C
85
L-threo-phenylserine
mutant D95N/E96S, pH 8.0, 30°C
96
L-threo-phenylserine
mutant D95G/E96G, pH 8.0, 30°C
100
L-threo-phenylserine
mutant D95Y/E96T, pH 8.0, 30°C
120
L-threo-phenylserine
mutant D95L, pH 8.0, 30°C
130
L-threo-phenylserine
mutant D95C, pH 8.0, 30°C
1900
L-threo-phenylserine
wild-type, pH 8.0, 30°C
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4.1
3-(ethylamino)phenol
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300
cleavage of L-threo-3-phenylserine
4.3
reaction with glycine and benzaldehyde
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7.5
reaction with glycine and benzyldehyde
8.5
reaction with L-threo-3-phenylserine
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cow
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brenda
enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
UniProt
brenda
enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
UniProt
brenda
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brenda
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brenda
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brenda
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brenda
strain 24-1
SwissProt
brenda
strain 24-1
SwissProt
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
additional information
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not: skeletal muscle, heart muscle, brain spleen, adrenals, red cells, plasma, serum
brenda
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G4CIW0_9NEIS
346
0
37614
TrEMBL
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G4CM77_9NEIS
353
0
38722
TrEMBL
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F5S9R0_KINKI
348
0
37971
TrEMBL
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D4YQK5_9MICO
346
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36436
TrEMBL
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Q59IT3_PSEPU
357
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38317
TrEMBL
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Q9A3V8_CAUVC
Caulobacter vibrioides (strain ATCC 19089 / CB15)
343
0
35908
TrEMBL
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37400
6 * 37400, calculation from nucleotide sequence
38000
6 * 38000, SDS-PAGE
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hexamer
6 * 38000, SDS-PAGE
hexamer
6 * 37400, calculation from nucleotide sequence
hexamer
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6 * 38000, SDS-PAGE
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hexamer
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6 * 37400, calculation from nucleotide sequence
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K213Q
loss of activity, disappearance of absorption maximum at 420 nm
K213Q
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loss of activity, disappearance of absorption maximum at 420 nm
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6.5 - 9.5
30°C, 10 min, stable
663708
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-20°C, pH 7.2, 10 mM TES buffer, 0.01% 2-mercaptoethanol, 0.05 mM pyridoxal 5'-phosphate, 30% glycerol, stable for several months
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overexpression in Escherichia coli
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Bruns, F.H.; Fiedler, L.
Enzymatic cleavage and synthesis of L-threo-beta-phenylserine and L-erythro-beta-phenylserine
Nature
181
1533-1534
1958
Bos taurus, Cavia porcellus, Ovis aries, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
brenda
Misono, H.; Maeda, H.; Tuda, K.; Ueshima, S.; Miyazaki, N.; Nagata, S.
Characterization of an inducible phenylserine aldolase from Pseudomonas putida 24-1
Appl. Environ. Microbiol.
71
4602-4609
2005
Pseudomonas putida (Q59IT3), Pseudomonas putida, Pseudomonas putida 24. Jan (Q59IT3)
brenda
Giger, L.; Toscano, M.; Bouzon, M.; Marlière, P.; Hilvert, D.
A novel genetic selection system for PLP-dependent threonine aldolases
Tetrahedron
68
7549-7557
2012
Caulobacter vibrioides (Q9A3V8), Caulobacter vibrioides CB15 (Q9A3V8)
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brenda
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