Information on EC 4.1.2.23 - 3-deoxy-D-manno-octulosonate aldolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.2.23
-
RECOMMENDED NAME
GeneOntology No.
3-deoxy-D-manno-octulosonate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-deoxy-D-manno-octulosonate = pyruvate + D-arabinose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
of an aldehyde, C-C bond cleavage
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SYSTEMATIC NAME
IUBMB Comments
3-deoxy-D-manno-octulosonate D-arabinose-lyase (pyruvate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-95-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-deoxy-D-manno-octulosonate
?
show the reaction diagram
3-deoxy-D-manno-octulosonate
pyruvate + D-arabinose
show the reaction diagram
3-deoxy-L-manno-octulosonate
pyruvate + L-arabinose
show the reaction diagram
-
-
-
-
r
D-sialic acid
N-acetyl-D-mannosamine + pyruvate
show the reaction diagram
-
the RS-aldolase has a 5.8fold higher cleavage rate toward 3-deoxy-L-manno-octulosonate than toward D-sialic acid
-
-
r
N-acetyl-D-mannosamine + pyruvate
D-sialic acid
show the reaction diagram
-
-
-
-
r
pyruvate + 2-deoxy-2-fluoro-D-arabinose
3,5-dideoxy-5-fluoro-alpha-D-manno-2-octulosonic acid
show the reaction diagram
pyruvate + 2-deoxy-D-ribose
3,5-dideoxy-alpha-D-manno-2-octulosonic acid
show the reaction diagram
pyruvate + 2-deoxy-D-ribose
3-deoxy-alpha-D-altro-2-octulosonic acid
show the reaction diagram
-
-
-
-
pyruvate + 5-azido-2,5-dideoxy-D-ribose
?
show the reaction diagram
-
at 15% of the activity with D-arabinose
-
-
-
pyruvate + D-altrose
?
show the reaction diagram
-
at 25% of the activity with D-arabinose
-
-
-
pyruvate + D-altrose
D-erythrose + D-glyceraldehyde
show the reaction diagram
pyruvate + D-arabinose
3-deoxy-D-manno-octulosonate
show the reaction diagram
pyruvate + D-erythrose
?
show the reaction diagram
pyruvate + D-glyceraldehyde
2-dehydo-3-deoxy-D-galactonate
show the reaction diagram
pyruvate + D-lyxose
?
show the reaction diagram
-
at 35% of the activity with D-arabinose
-
-
-
pyruvate + D-ribose
3-deoxy-L-glycero-L-galacto-2-nonulosonic acid
show the reaction diagram
-
-
-
-
pyruvate + D-ribose
?
show the reaction diagram
pyruvate + D-threose
?
show the reaction diagram
pyruvate + L-arabinose
3-deoxy-L-manno-octulosonate
show the reaction diagram
-
-
-
-
r
pyruvate + L-glyceraldehyde
2-keto-3-deoxy-L-gluconic acid
show the reaction diagram
pyruvate + L-mannose
3-deoxy-alpha-D-arabino-2-heptulosonic acid
show the reaction diagram
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-deoxy-D-manno-octulosonate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate
-
one sulfate ion is found adjacent to the side chain of Lys-165 in the active site and another one on the surface of RS-aldolase
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 6
3-deoxy-D-manno-octulosonate
199
L-arabinose
-
in 50 mM Tris-HCl, pH 7.8, at 37°C
181
N-acetyl-D-mannosamine
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in 50 mM Tris-HCl, pH 7.8, at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.3
L-arabinose
Escherichia coli
-
in 50 mM Tris-HCl, pH 7.8, at 37°C
1.1
N-acetyl-D-mannosamine
Escherichia coli
-
in 50 mM Tris-HCl, pH 7.8, at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
L-arabinose
Escherichia coli
-
in 50 mM Tris-HCl, pH 7.8, at 37°C
206
0.0055
N-acetyl-D-mannosamine
Escherichia coli
-
in 50 mM Tris-HCl, pH 7.8, at 37°C
1064
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 7.8
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pH 6.3: about 45% of maximal activity, pH 7.8: about 65% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with hydroxypyruvate, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate and 0.1 M Bis-Tris, pH 6.5
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10-20% loss of activity, even when the preparations are repeatedly thawed and refrozen several times over a period of one year
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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