Information on EC 4.1.2.18 - 2-dehydro-3-deoxy-L-pentonate aldolase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.1.2.18
-
RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-L-pentonate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-Dehydro-3-deoxy-L-pentonate = pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
condensation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
-
Fructose and mannose metabolism
-
L-arabinose degradation IV
-
SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-L-pentonate glycolaldehyde-lyase (pyruvate-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-keto-3-deoxy-D-xylonate aldolase
-
-
-
-
2-keto-3-deoxy-L-arabonate aldolase
-
-
-
-
2-keto-3-deoxy-L-pentonate aldolase
-
-
-
-
3-Deoxy-D-penulosonic acid aldolase
-
-
-
-
aldolase, 2-keto-3-deoxy-L-arabonate
-
-
-
-
aldolase, 2-keto-3-deoxy-L-pentonate
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9076-49-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 61A76
-
-
Manually annotated by BRENDA team
Bradyrhizobium japonicum 61A76
strain 61A76
-
-
Manually annotated by BRENDA team
no activity in Azospirillum brasiliense
-
-
-
Manually annotated by BRENDA team
no activity in Rhizobium leguminosarum
strain DL-14
-
-
Manually annotated by BRENDA team
no activity in Rhizobium leguminosarum DL-14
strain DL-14
-
-
Manually annotated by BRENDA team
no activity in Rhizobium phaseoli
strain K26
-
-
Manually annotated by BRENDA team
no activity in Rhizobium phaseoli K26
strain K26
-
-
Manually annotated by BRENDA team
no activity in Rhizobium trifolii
strain TIL
-
-
Manually annotated by BRENDA team
no activity in Rhizobium trifolii TIL
strain TIL
-
-
Manually annotated by BRENDA team
strain MSU-1
-
-
Manually annotated by BRENDA team
Pseudomonas sp. MSU-1
strain MSU-1
-
-
Manually annotated by BRENDA team
strain 32H1
-
-
Manually annotated by BRENDA team
strain BTAi 1
-
-
Manually annotated by BRENDA team
Rhizobium sp. 32H1
strain 32H1
-
-
Manually annotated by BRENDA team
Rhizobium sp. BTAi 1
strain BTAi 1
-
-
Manually annotated by BRENDA team
strain L5-30
-
-
Manually annotated by BRENDA team
Sinorhizobium meliloti L5-30
strain L5-30
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Keto-3-deoxy-D-fuconate
Pyruvate + D-lactaldehyde
show the reaction diagram
-
-
-
-
2-Keto-3-deoxy-D-fuconate
Pyruvate + D-lactaldehyde
show the reaction diagram
-
-
-
-
-
2-Keto-3-deoxy-D-fuconate
Pyruvate + D-lactaldehyde
show the reaction diagram
Pseudomonas sp. MSU-1
-
-
-
-
2-Keto-3-deoxy-D-fuconate
Pyruvate + D-lactaldehyde
show the reaction diagram
Pseudomonas sp. MSU-1
-
-
-
-
-
2-Keto-3-deoxy-D-xylonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
r, stereospecific reaction, the product does not serve as a substrate for the formation of 2-keto-3-deoxy-L-arabonate
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
Pseudomonas sp. MSU-1
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
Pseudomonas sp. MSU-1
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
Rhizobium sp. 32H1, Rhizobium sp. 32111, Bradyrhizobium japonicum 61A76, Sinorhizobium meliloti L5-30
-
-
-
-
-
2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
Rhizobium sp. BTAi 1
-
-
-
-
-
additional information
?
-
-
enzyme activity increases 3fold by growth on D-fucose or L-arabinose
-
-
-
additional information
?
-
-
induced by growth on arabinose
-
-
-
additional information
?
-
-
enzyme functions in the metabolism of L-arabinose and D-fucose
-
-
-
additional information
?
-
Pseudomonas sp. MSU-1
-
enzyme activity increases 3fold by growth on D-fucose or L-arabinose
-
-
-
additional information
?
-
Pseudomonas sp. MSU-1
-
enzyme functions in the metabolism of L-arabinose and D-fucose
-
-
-
additional information
?
-
Rhizobium sp. BTAi 1
-
induced by growth on arabinose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme activity increases 3fold by growth on D-fucose or L-arabinose
-
-
-
additional information
?
-
-
induced by growth on arabinose
-
-
-
additional information
?
-
-
enzyme functions in the metabolism of L-arabinose and D-fucose
-
-
-
additional information
?
-
Pseudomonas sp. MSU-1
-
enzyme activity increases 3fold by growth on D-fucose or L-arabinose
-
-
-
additional information
?
-
Pseudomonas sp. MSU-1
-
enzyme functions in the metabolism of L-arabinose and D-fucose
-
-
-
additional information
?
-
Rhizobium sp. BTAi 1
-
induced by growth on arabinose
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
95% of the activation with Mn2+; divalent cation required
Co2+
-
76% of the activation with Mn2+; divalent cation required
Mg2+
-
54% of the activity with Mn2+; divalent cation required
Mg2+
-
33% of the activation with Mn2+; divalent cation required
Mn2+
-
divalent cation required; most effective
Ni2+
-
45% of the activity with Mn2+; divalent cation required
Ni2+
-
divalent cation required; no activation
Zn2+
-
divalent cation required, 15% of the activation with Mn2+
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.9
-
2-Keto-3-deoxy-D-fuconate
-
-
0.97
-
2-keto-3-deoxy-D-xylonate
-
-
1.8
-
2-Keto-3-deoxy-L-arabonate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.16
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
8.2
-
-
7.8
8.3
-
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
53
-
-
half-life: 30 min
55
-
-
half-life: 15 min
55
-
-
half-life: 2 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-thioethanol stabilizes during purification
-
thiols stabilize during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 0.1 M bicine, 0.14 mM 2-thioethanol, 3 weeks, 70% loss of activity
-
-20°C, 2 weeks, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE