Information on EC 4.1.2.18 - 2-dehydro-3-deoxy-L-pentonate aldolase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
4.1.2.18
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RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-L-pentonate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-Dehydro-3-deoxy-L-pentonate = pyruvate + glycolaldehyde
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
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degradation of pentoses
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L-arabinose degradation IV
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-L-pentonate glycolaldehyde-lyase (pyruvate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9076-49-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 61A76
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Manually annotated by BRENDA team
no activity in Azospirillum brasiliense
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-
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Manually annotated by BRENDA team
no activity in Rhizobium leguminosarum
strain DL-14
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-
Manually annotated by BRENDA team
no activity in Rhizobium leguminosarum DL-14
strain DL-14
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Manually annotated by BRENDA team
no activity in Rhizobium phaseoli
strain K26
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Manually annotated by BRENDA team
no activity in Rhizobium phaseoli K26
strain K26
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Manually annotated by BRENDA team
no activity in Rhizobium trifolii
strain TIL
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Manually annotated by BRENDA team
no activity in Rhizobium trifolii TIL
strain TIL
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Manually annotated by BRENDA team
strain MSU-1
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Manually annotated by BRENDA team
strain 32H1
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Manually annotated by BRENDA team
Rhizobium sp. BTAi 1
strain BTAi 1
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Manually annotated by BRENDA team
strain L5-30
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Manually annotated by BRENDA team
strain L5-30
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Keto-3-deoxy-D-fuconate
Pyruvate + D-lactaldehyde
show the reaction diagram
2-Keto-3-deoxy-D-xylonate
Pyruvate + glycolaldehyde
show the reaction diagram
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r, stereospecific reaction, the product does not serve as a substrate for the formation of 2-keto-3-deoxy-L-arabonate
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2-Keto-3-deoxy-L-arabonate
Pyruvate + glycolaldehyde
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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divalent cation required; most effective
Zn2+
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divalent cation required, 15% of the activation with Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9
2-Keto-3-deoxy-D-fuconate
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0.97
2-keto-3-deoxy-D-xylonate
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1.8
2-Keto-3-deoxy-L-arabonate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.2
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7.8 - 8.3
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53
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half-life: 30 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-thioethanol stabilizes during purification
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thiols stabilize during purification
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.1 M bicine, 0.14 mM 2-thioethanol, 3 weeks, 70% loss of activity
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-20°C, 2 weeks, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE