Information on EC 4.1.1.92 - indole-3-carboxylate decarboxylase

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The expected taxonomic range for this enzyme is: Arthrobacter nicotianae

EC NUMBER
COMMENTARY
4.1.1.92
-
RECOMMENDED NAME
GeneOntology No.
indole-3-carboxylate decarboxylase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
indole-3-carboxylate = indole + CO2
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
indole-3-carboxylate carboxy-lyase
Activated by Zn2+, Mn2+ or Mg2+.
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Arthrobacter nicotianae FI1612
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methylindole + CO2
2-methylindole-3-carboxylate
show the reaction diagram
Arthrobacter nicotianae, Arthrobacter nicotianae FI1612
-
-
-
-
?
indole-3-carboxylate
indole + CO2
show the reaction diagram
Arthrobacter nicotianae, Arthrobacter nicotianae FI1612
-
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole-3-carboxylate
indole + CO2
show the reaction diagram
Arthrobacter nicotianae, Arthrobacter nicotianae FI1612
-
-
-
-
r
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
the enzyme does not need any cofactor for the decarboxylation and carboxylation reaction
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
1 mM, 1.3fold activation
Mg2+
-
1 mM, 1.9fold activation
Mn2+
-
1 mM, 2.1fold activation
Zn2+
-
1 mM, 2.1fold activation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
1 mM, 85% inhibition
2,2'-Bipyridyl
-
1 mM, 72% inhibition
8-hydroxyquinoline
-
1 mM, 49% inhibition
AgNO3
-
1 mM, 50% inhibition
CuCl2
-
1 mM, complete inhibition
HgCl2
-
1 mM, 69% inhibition
hydroxylamine
-
1 mM, 35% inhibition
N-ethylmaleimide
-
1 mM, 97% inhibition
NaN3
-
1 mM, 14% inhibition
phenylhydrazine
-
1 mM, 87% inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.719
-
Indole
-
pH 7.0, 30C
0.631
-
indole-3-carboxylate
-
pH 7.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.224
-
-
pH 7.0, 30C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
decarboxylation, potassium phosphate buffer
8
-
-
carboxylation, Tris-HCl buffer, 100 mM
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
decarboxylation. For the carboxylation reaction, temperatures over 30C are not appropriate
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
258000
-
-
gel filtration
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
30 min, stable
30
-
-
30 min, 13% loss of initial activity
40
-
-
30 min, 35% loss of initial activity
50
-
-
30 min, 65% loss of initial activity
60
-
-
30 min, complete loss of initial activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, cell extract, 80% of the initial activity is lost after 4 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
induced specifically by indole-3-carboxylate, but not by indole
-
induced specifically by indole-3-carboxylate, but not by indole
Arthrobacter nicotianae FI1612
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-