Information on EC 4.1.1.78 - acetylenedicarboxylate decarboxylase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY hide
4.1.1.78
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RECOMMENDED NAME
GeneOntology No.
acetylenedicarboxylate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetylenedicarboxylate + H2O = pyruvate + CO2
show the reaction diagram
the mechanism appears to involve hydration of the acetylene and decarboxylation of the oxaloacetic acid formed, although free oxaloacetate is not an intermediate. It is thus analogous to EC 4.2.1.71, acetylenecarboxylate hydratase, in its mechanism. Formerly EC 4.2.1.72
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition of H2O
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decarboxylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pyruvate metabolism
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SYSTEMATIC NAME
IUBMB Comments
acetylenedicarboxylate carboxy-lyase (pyruvate-forming)
The mechanism appears to involve hydration of the acetylene and decarboxylation of the oxaloacetic acid formed, although free oxaloacetate is not an intermediate. It is thus analogous to EC 4.2.1.27 (acetylenecarboxylate hydratase) in its mechanism.
CAS REGISTRY NUMBER
COMMENTARY hide
72561-10-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Clostridium cylindrosporum
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Manually annotated by BRENDA team
no activity in Clostridium kluyveri
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Manually annotated by BRENDA team
no activity in Escherichia coli
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Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens
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Manually annotated by BRENDA team
no activity in Rattus norvegicus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetylene dicarboxylate
pyruvate + CO2
show the reaction diagram
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ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetylene dicarboxylate
pyruvate + CO2
show the reaction diagram
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ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no requirement
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al(NO3)3
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Fe(NO3)3
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p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
acetylene dicarboxylate
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23°C, pH 7.3
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 7.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
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enzyme assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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highest stability at 3°C for 17 h
648381
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
loss of stabililty by freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.005 mM 2-mercaptoethanol, 10 days, less than 10% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE