Information on EC 4.1.1.74 - Indolepyruvate decarboxylase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.1.1.74
-
RECOMMENDED NAME
GeneOntology No.
Indolepyruvate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
mechanism
-
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
mechanism
-
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
residues E468, D29, and H115 are involved in binding of all substrates and decarboxlation of the respective tetrahedral thiamine-diphosphate-substrate adducts. Residue E383 is central to substrate specificity
-
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
mechanism
Sulfolobus sp. 7
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-tryptophan degradation VII (via indole-3-pyruvate)
-
-
L-tryptophan degradation VIII (to tryptophol)
-
-
Metabolic pathways
-
-
methionine metabolism
-
-
Tryptophan metabolism
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-(indol-3-yl)pyruvate carboxy-lyase [(2-indol-3-yl)acetaldehyde-forming]
Thiamine diphosphate- and Mg2+-dependent. More specific than EC 4.1.1.1 pyruvate decarboxylase
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Decarboxylase, indolepyruvate
-
-
-
-
Decarboxylase, indolepyruvate (Enterobacter agglomerans clone pMB2 gene ipdC reduced)
-
-
-
-
GenBank L80006-derived protein GI 1507711
-
-
-
-
Indole-3-pyruvate decarboxylase
-
-
-
-
Indolepyruvate decarboxylase
-
-
-
-
Indolepyruvate decarboxylase (Enterobacter herbocola strain 299R clone pMB2 gene ipdC reduced)
-
-
-
-
Indolepyruvic acid decarboxylase
-
-
-
-
IpdC
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
183213-32-3
-
9074-92-4
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain SM and its variants, strain Sp7, and strain Sp245
UniProt
Manually annotated by BRENDA team
strain SM and strain SMIT568s10
UniProt
Manually annotated by BRENDA team
Azospirillum brasilense SM
-
-
-
Manually annotated by BRENDA team
Azospirillum brasilense Sp7
Sp7
-
-
Manually annotated by BRENDA team
UW5, previously misidentified as Pseudomonas putida and strain J3, J35, J51, and J55
UniProt
Manually annotated by BRENDA team
Enterobacter cloacae ATCC 13047
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens Psd
-
-
-
Manually annotated by BRENDA team
strain 7
-
-
Manually annotated by BRENDA team
Sulfolobus sp. 7
strain 7
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
C5ARG4
an ipdC-knockout mutant grown in the presence of tryptophan has decreased indole-3-acetic acid level (46% of wild type strain)
metabolism
C5ARG4
IpdC id involved in indole-3-acetic acid biosynthesis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
P51852
-
-
-
?
(indole-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
-
?
2-oxoglutarate
?
show the reaction diagram
Sulfolobus sp., Sulfolobus sp. 7
-
-
-
?
2-oxoisovalerate
?
show the reaction diagram
Sulfolobus sp., Sulfolobus sp. 7
-
-
-
?
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
-
-
-
-
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
Azospirillum brasilense Sp7
-
-
-
-
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
C5ARG4
3-(indol-3-yl)pyruvate is decarboxylated with the highest catalytic efficiency (kcat/Km)
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
Enterobacter cloacae ATCC 13047
-
-
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
Sulfolobus sp. 7
-
-
-
?
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
a mutant defective in this enzyme is significantly reduced in indole-3-acetic acid production
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
upregulation of expression by indole-3-acetic acid and synthetic auxins
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
key enzyme for indole-3-acetic acid biosynthesis
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
rate-limiting step in the indole-3-pyruvic acid pathway
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
a mutant defective in this enzyme is significantly reduced in indole-3-acetic acid production, enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
P51852
-
-
-
?
4-bromo-benzoylformic acid
4-bromobenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-chloro-benzoylformic acid
4-chlorobenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-ethyl-benzoylformic acid
4-ethylbenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-fluoro-benzoylformic acid
4-fluorobenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-hydroxyphenylpyruvate
(4-hydroxyphenyl)acetaldehyde + CO2
show the reaction diagram
C5ARG4
-
-
-
-
4-methoxy-benzoylformic acid
4-methoxybenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-methyl-benzoylformic acid
4-methylbenzaldehyde + CO2
show the reaction diagram
-
-
-
?
4-nitro-benzoylformic acid
4-nitrobenzaldehyde + CO2
show the reaction diagram
-
-
-
?
benzaldehyde
?
show the reaction diagram
-
-
-
?
Benzoylformate
Benzaldehyde + CO2
show the reaction diagram
C5ARG4
the enzyme shows the highest kcat value for benzoylformate
-
-
?
benzoylformic acid
benzaldehyde + CO2
show the reaction diagram
-
-
-
?
benzoylformic acid
benzaldehyde + CO2
show the reaction diagram
-
-
-
-
?
glyceraldehyde
?
show the reaction diagram
-
-
-
?
indole-3-acetaldehyde
?
show the reaction diagram
Sulfolobus sp., Sulfolobus sp. 7
-
-
-
?
phenylacetaldehyde
?
show the reaction diagram
-
-
-
?
Pyruvate
Acetaldehyde + CO2
show the reaction diagram
-
-
-
?
Pyruvate
Acetaldehyde + CO2
show the reaction diagram
-
-
-
-
?
Pyruvate
Acetaldehyde + CO2
show the reaction diagram
-
-
-
?
Pyruvate
Acetaldehyde + CO2
show the reaction diagram
C5ARG4
-
-
-
?
Pyruvate
Acetaldehyde + CO2
show the reaction diagram
Sulfolobus sp. 7
-
-
-
?
Pyruvic acid
Acetaldehyde + CO2
show the reaction diagram
-
decarboxylation at 19% of the indole-3-acetic acid decarboxylation
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
P51852
-
-
-
?
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
-
?
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
a mutant defective in this enzyme is significantly reduced in indole-3-acetic acid production
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
upregulation of expression by indole-3-acetic acid and synthetic auxins
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
key enzyme for indole-3-acetic acid biosynthesis
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
rate-limiting step in the indole-3-pyruvic acid pathway
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
P51852
-
-
-
?
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
-
a mutant defective in this enzyme is significantly reduced in indole-3-acetic acid production, enzyme is involved in indole-3-acetic acid synthesis
-
-
-
3-(indol-3-yl)pyruvate
2-(indol-3-yl)acetaldehyde + CO2
show the reaction diagram
Enterobacter cloacae ATCC 13047
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
biotin
-
binds possibly to beta subunit
thiamine diphosphate
-
0.1 mM, 8fold increase in activity. 39fold increase in activity in presence of 0.1 mM thiamine diphosphate and 5 mM Mg2+
thiamine diphosphate
-
-
thiamine diphosphate
C5ARG4
dependent on, IpdC is preincubated with 15mM thiamine diphosphate/Mg2+ in 10 mM Mes-NaOH at 25C for 30 min to saturate the enzyme with cofactor
FMN
-
1.9 mol per mol of enzyme
additional information
-
no thiamine diphosphate
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
-
8.7 mol per mol of enzyme
Mg2+
-
5 mM, 10fold increase in activity. 39fold increase in activity in presence of 0.1 mM thiamine diphosphate and 5 mM Mg2+. One of the roles of Mg2+ is to facilitate the binding of thiamin diphosphate to the enzyme by formation of a thiamine diphosphate complex
Mg2+
-
required
Mg2+
C5ARG4
required, IpdC is preincubated with 15mM thiamine diphosphate/Mg2+ in 10 mM Mes-NaOH at 25C for 30 min to saturate the enzyme with cofactor
Mo2+
-
0.8 mol per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-oxoglutaric acid
-
-
3-(Indol-3-yl)pyruvate
C5ARG4
substrate inhibition
3-phenylpyruvic acid
-
competitive against indole-3-acetic acid
4-hydroxyphenylpyruvate
C5ARG4
substrate inhibition
Pyruvic acid
-
-
SMIT568s10
P51852
knock-out strain, SMIT568s10 which shows a significant (50%) decrease in the levels of (indole-3-yl)acetic acid, (indole-3-yl)acetaldehyde and tryptophol compared to the wild type strain SM
-
benzoylformate
C5ARG4
substrate inhibition
additional information
C5ARG4
no inhibition by pyruvate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
indole-3-acetic acid
P51852
expression of ipdC is upregulated by indole-3-acetic acid, other auxins, and nutrient stress
L-phenylalanine
P23234
expression increases in response to
L-tryptophan
P51852
at low concentrations, Trp does not influence ipdC expression but 2fold induction is observed at 2.5 mM Trp
L-tryptophan
P23234
expression increases in response to
L-tyrosine
P23234
expression increases in response to
tryptophan
P51852
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0045
3-(Indol-3-yl)pyruvate
-
mutant E52D, pH 6.5, 30C
0.015
3-(Indol-3-yl)pyruvate
-
-
0.018
3-(Indol-3-yl)pyruvate
-
mutant E468D, pH 6.5, 30C
0.02
3-(Indol-3-yl)pyruvate
-
wild-type, pH 6.5, 30C
0.024
3-(Indol-3-yl)pyruvate
-
mutant Q383T, pH 6.5, 30C
0.025
3-(Indol-3-yl)pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
0.032
3-(Indol-3-yl)pyruvate
-
mutant H115K, pH 6.5, 30C
0.019
4-bromo-benzoylformic acid
-
pH 6.5, 30C
0.048
4-chloro-benzoylformic acid
-
pH 6.5, 30C
0.111
4-ethyl-benzoylformic acid
-
pH 6.5, 30C
0.62
4-fluoro-benzoylformic acid
-
pH 6.5, 30C
5.86
4-hydroxyphenylpyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
1.05
4-methoxy-benzoylformic acid
-
pH 6.5, 30C
0.127
4-methyl-benzoylformic acid
-
pH 6.5, 30C
0.005
4-nitro-benzoylformic acid
-
pH 6.5, 30C
0.395
benzaldehyde
-
70C, pH 8.0
7.3
benzoylformate
C5ARG4
at pH 6.5, temperature not specified in the publication
0.024
benzoylformic acid
-
mutant D29E, pH 6.5, 30C
0.14
benzoylformic acid
-
mutant E468D, pH 6.5, 30C
0.27
benzoylformic acid
-
mutant H115K, pH 6.5, 30C
1.65
benzoylformic acid
-
pH 6.5, 30C
1.65
benzoylformic acid
-
wild-type, pH 6.5, 30C
1.71
benzoylformic acid
-
mutant Q383T, pH 6.5, 30C
2.72
benzoylformic acid
-
mutant E52D, pH 6.5, 30C
0.042
glyceraldehyde
-
70C, pH 8.0
0.009
Indole-3-acetaldehyde
-
70C, pH 8.0
0.02
Indole-3-pyruvate
-
pH 6.5, 30C
0.788
Indole-3-pyruvate
-
70C, pH 8.0
0.105
phenylacetaldehyde
-
70C, pH 8.0
1.66
phenylpyruvate
-
70C, pH 8.0
0.63
pyruvate
-
mutant D29E, pH 6.5, 30C
0.73
pyruvate
-
mutant Q383T, pH 6.5, 30C
0.77
pyruvate
-
mutant E52D, pH 6.5, 30C
3.16
pyruvate
-
mutant E468D, pH 6.5, 30C
3.38
pyruvate
-
pH 6.5, 30C
3.38
pyruvate
-
wild-type, pH 6.5, 30C
3.48
pyruvate
-
mutant H115K, pH 6.5, 30C
43.3
pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
2.5
Pyruvic acid
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.04
3-(Indol-3-yl)pyruvate
-
mutant E468D, pH 6.5, 30C
0.1
3-(Indol-3-yl)pyruvate
-
mutant E52D, pH 6.5, 30C
0.31
3-(Indol-3-yl)pyruvate
-
mutant H115K, pH 6.5, 30C
0.64
3-(Indol-3-yl)pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
0.98
3-(Indol-3-yl)pyruvate
-
wild-type, pH 6.5, 30C
2.05
3-(Indol-3-yl)pyruvate
-
mutant Q383T, pH 6.5, 30C
1.65
4-hydroxyphenylpyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
31.4
benzoylformate
C5ARG4
at pH 6.5, temperature not specified in the publication
0.01
benzoylformic acid
-
mutant D29E, pH 6.5, 30C
0.07
benzoylformic acid
-
mutant E468D, pH 6.5, 30C
0.29
benzoylformic acid
-
mutant H115K, pH 6.5, 30C
0.87
benzoylformic acid
-
mutant E52D, pH 6.5, 30C
2.25
benzoylformic acid
-
mutant Q383T, pH 6.5, 30C
11.6
benzoylformic acid
-
wild-type, pH 6.5, 30C
0.016
pyruvate
-
mutant H115K, pH 6.5, 30C
0.019
pyruvate
-
mutant D29E, pH 6.5, 30C
0.06
pyruvate
-
mutant E52D, pH 6.5, 30C
0.069
pyruvate
-
mutant E468D, pH 6.5, 30C
0.88
pyruvate
-
wild-type, pH 6.5, 30C
0.89
pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
2.06
pyruvate
-
mutant Q383T, pH 6.5, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
25.6
3-(Indol-3-yl)pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
3503
0.28
4-hydroxyphenylpyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
423
4.3
benzoylformate
C5ARG4
at pH 6.5, temperature not specified in the publication
613
0.021
pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
31
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.73
3-(Indol-3-yl)pyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
22.11
4-hydroxyphenylpyruvate
C5ARG4
at pH 6.5, temperature not specified in the publication
3.4
benzoylformate
C5ARG4
at pH 6.5, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.102
P51852
strain SMIT568s10, product: (indole-3-yl)ethanol, substrate: -Trp
0.217
P51852
strain SMIT568s10, product: (indole-3-yl)acetaldehyde, substrate: -Trp
0.268
P51852
strain SM, product: (indole-3-yl)ethanol, substrate: -Trp
0.615
P51852
strain SM, product: (indole-3-yl)acetaldehyde , substrate: -Trp
0.626
P51852
strain SMIT568s10, product: (indole-3-yl)ethanol, substrate: +Trp
1.006
P51852
strain SMIT568s10, product: (indole-3-yl)acetaldehyde, substrate: +Trp
1.252
P51852
strain SM, product: (indole-3-yl)ethanol, substrate: +Trp
2.3
P51852
strain SM, product: (indole-3-yl)acetaldehyde , substrate: +Trp
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.4 - 6.6
-
-
8
-
70% loss of activity at pH 7.0, 15% loss of activity at pH 9
8.2
P51852
external pH influences ipdC expression as maximum expression is observed at the alkaline pH of 8.2 (69% increase) while highly acidic pH decreases the expression level. At pH 4.2, expression is ca. 32% of that observed at pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
P51852
temperatures on either side of the growth optimum (30C) are found to alter ipdC expression significantly. At 25C a 48% increase and at 37C an 86% increase are noted
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
57500
P23234
tyrR insertional mutant
692883
63380
-
SDS-PAGE, purified, corresponding closely to the expected molecular mass
693596
240000
-
gel filtration
4826
245000
-
gel filtration
651152
245000
C5ARG4
holoenzyme, gel filtration
714125
280000
-
gel filtration
651291
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 60000, SDS-PAGE
?
Enterobacter cloacae ATCC 13047
-
x * 60000, SDS-PAGE
-
hexamer
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE
hexamer
Sulfolobus sp. 7
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE
-
tetramer
-
4 * 60000, SDS-PAGE
tetramer
-
dimer of dimers, crystallographic data
homotetramer
C5ARG4
4 * 60000, SDS-PAGE
additional information
-
at pH-values between pH 5.6 and 6.0 the tetrameric form dominates, at pH values above 8.0, the monomeric form is present
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
P51852
expression of ipdC is upregulated by an increase in pH
692100
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
24 h, in presence of 1 mM thiamine diphosphate and 5 mM MgCl2, stable
4826
50
-
30 min, in presence of 1 mM thiamine diphosphate and 5 mM MgCl2, stable
4826
70
-
30 min, pH 7.0, almost stable
651291
90
-
30 min, 22% inactivation
651291
100
-
30 min, 97% inactivation
651291
additional information
P51852
expression of ipdC is upregulated by temperature
692100
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate stabilizes
-
less stable at a high potassium phosphate concentration
-
slightly stabilized in presence of 5 mM MgCl2
-
stabilized by addition of 1 mM thiamine diphosphate
-
thiamine diphosphate stabilizes
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ethanol
-
rather stable in ethanol
Ethyl acetate
-
rather stable in ethyl acetate
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
quite stable at 40C without further additions
-
-20C, 20% (v/v) glycerol with addition of thiamine diphosphate/Mg2+, six months, the enzyme remains quite stable
C5ARG4
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli cells
-
Ni-NTA column chromatography, gel filtration
C5ARG4
recombinant protein, purified to homogeneity using affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Pseudomonas fluorescens strain Psd
-
heterologous overexpression of a functional iaaC gene from strain Sp245 in strain SM confirms its role in controlling IAA biosynthesis and lowering ipdC expression which may be effected by dissociating indole-3-acetic acid-transcriptional regulator interactions in the 5'region
P51852
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli BL21(DE3) cells
C5ARG4
cloned in Escherichia coli DH5alpha, recombinant protein expressed in Escherichia coli BL21 (DE3) as a glutathione S-transferase (GST)-fusion protein
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D29E
-
significant decrease in Km-values
E468D
-
slight decrease in Km-values
E52D
-
decrease in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, increase in Km-value for benzoylformic acid
H115K
-
slight increase in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, decrease in Km-value for benzoylformic acid
Q383T
-
decrease in Km-value for pyruvate
tyrR insertional mutant
P23234
activity of tyrR in Enterobacter cloacae UW5 is abolished by insertion of a tetracycline resistance cassette into the coding sequence, creating Enterobacter cloacae J35
additional information
P51852
ipdC gene knock-out, strain SMIT568s10 which shows a significant (50%) decrease in the levels of indole-3-acetic acid, indole-3-acetaldehyde and tryptophol compared to the wild type strain SM. Reduction in the plant growth promoting response of strain SMIT568s10 is observed in terms of root length and lateral root proliferation as well as the total dry weight of the treated plants