Information on EC 4.1.1.73 - tartrate decarboxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.1.73
-
RECOMMENDED NAME
GeneOntology No.
tartrate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R,R)-tartrate = D-glycerate + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
-
redox reaction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R,R)-tartrate carboxy-lyase (D-glycerate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
124248-30-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 5D1A, belongs to group Ve-2
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
L-tartrate
D-glycerate + CO2
show the reaction diagram
meso-tartrate
D-glycerate + CO2
show the reaction diagram
oxaloacetate + NAD+
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-tartrate
D-glycerate + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
-
requirement, less effective than NAD+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation
Cu2+
-
slight activation
K+
-
activation, above 40 mM
Mn2+
-
activation
NH4+
-
slight activation
Ni2+
-
slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
strong
D-glycerate
-
product inhibition, 10 mM, 65% inhibition
D-malate
-
10 mM, 83% inhibition
L-Glycerate
-
10 mM, 63% inhibition
L-malate
-
10 mM, 29% inhibition
L-Tartrate
meso-tartrate
-
10 mM, 14% inhibition
Ni2+
-
weak
succinate
-
10 mM, 28% inhibition
Zn2+
-
strong
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
L-Tartrate
-
pH 7.5, 30C
1.1
NAD+
-
pH 7.5, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8
-
pH 7.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
half-maximal activity at pH 5.5 and 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
D-glycerate formation with whole cells
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
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isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
4 * 40000, SDS-PAGE
148000
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HPLC gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
30 min at 60C, inactivation
648380
7
-
30 min at 60C, 23% loss of activity
648380
7.5
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30 min at 60C, in 20 mM Tris-HCl buffer, 0.2 mM MgCl2, 5% loss of activity
648380
8
-
30 min at 60C, 13% loss of activity
648380
9
-
30 min at 60C, 57% loss of activity
648380
9.5
-
30 min at 60C, inactivation
648380
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, stable
60
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30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 5% loss of activity, inactivation at pH 6.5 and 9.5
65
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 29% loss of activity
70
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50% glycerol, 3 months, stable, without glycerol, 1 month, 40% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
about 11fold
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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