Information on EC 4.1.1.61 - 4-Hydroxybenzoate decarboxylase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.1.1.61
-
RECOMMENDED NAME
GeneOntology No.
4-Hydroxybenzoate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4-hydroxybenzoate = phenol + CO2
show the reaction diagram
-
-
-
-
4-hydroxybenzoate = phenol + CO2
show the reaction diagram
biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction
-
4-hydroxybenzoate = phenol + CO2
show the reaction diagram
mechanism
-
4-hydroxybenzoate = phenol + CO2
show the reaction diagram
biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction
Pseudomonas sp. K172
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
decarboxylation
-, C0H3U9
-
PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzoate carboxy-lyase (phenol-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4-hydroxybenzoate DC
C0H3U9
-
4-hydroxybenzoate decarboxylase
C0H3U9
-
Decarboxylase, p-hydroxybenzoate
-
-
-
-
HBDC
Chlamydia pneumoniae AR39
-
-
-
p-Hydroxybenzoate decarboxylase
-
-
-
-
Phenol carboxylase
-
-
-
-
vanillate/4-hydroxybenzoate decarboxylase
C0H3U9
-
CAS REGISTRY NUMBER
COMMENTARY
37290-53-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ATCC 6051
UniProt
Manually annotated by BRENDA team
strain AR39
-
-
Manually annotated by BRENDA team
Chlamydia pneumoniae AR39
strain AR39
-
-
Manually annotated by BRENDA team
in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
Manually annotated by BRENDA team
Aerobacter aerogenes
-
-
Manually annotated by BRENDA team
strain P240
-
-
Manually annotated by BRENDA team
Enterobacter cloacae P240
strain P240
-
-
Manually annotated by BRENDA team
in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
Manually annotated by BRENDA team
strain K172
-
-
Manually annotated by BRENDA team
Pseudomonas sp. K172
strain K172
-
-
Manually annotated by BRENDA team
-
Q4R101 and Q9S4M7 and Q4R102
UniProt
Manually annotated by BRENDA team
Clostridium hydroxybenzoicum, strain JW/Z-1T
-
-
Manually annotated by BRENDA team
Clostridium-like strain 6
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
show the reaction diagram
-
-
-
-
-
3,4-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
show the reaction diagram
-
slow reaction
-
?
3,4-dihydroxybenzoate
benzene-1,2-diol + CO2
show the reaction diagram
Enterobacter cloacae, Enterobacter cloacae P240
-
activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate
-
-
?
3,5-Dihydroxybenzoate
1,3-Dihydroxybenzene + CO2
show the reaction diagram
-
-
-
-
-
3,5-Dimethoxy-4-hydroxybenzoate
1,3-Dimethoxy-2-hydroxybenzene + CO2
show the reaction diagram
-
-
-
-
-
3-Chloro-4-hydroxybenzoate
1-Chloro-2-hydroxybenzene + CO2
show the reaction diagram
-
-
-
-
-
3-methoxy-4-hydroxybenzoate
2-methoxyphenol + CO2
show the reaction diagram
-, C0H3U9
vanillate
guaiacol
-
?
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
-, C0H3U9
-
-
-
?
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
Enterobacter cloacae P240
-
-
-
-
r
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
Pseudomonas sp. K172
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
Pseudomonas sp. K172
-
-
-
-
4-Hydroxybenzoate
Phenol + CO2
show the reaction diagram
Chlamydia pneumoniae AR39
-
-
-
-
r
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
-
-
-
-
-
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
-
-
-
-
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
-
carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19%
-
-
?
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
-
weak reaction
-
-
r
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
Enterobacter cloacae P240
-
carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19%
-
-
?
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
Pseudomonas sp. K172
-
-
-
-
-
Phenol + CO2
4-Hydroxybenzoate
show the reaction diagram
Chlamydia pneumoniae AR39
-
weak reaction
-
-
r
Phenol + CO2
?
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. K172
-
first step in the anaerobic metabolism of phenol
-
-
-
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
show the reaction diagram
-
-
-
-
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
show the reaction diagram
-
-
-
-
-
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
show the reaction diagram
Pseudomonas sp. K172
-
-
-
-
Phenylphosphate + CO2
4-Hydroxybenzoate + phosphate
show the reaction diagram
Pseudomonas sp. K172
-
-
-
-
-
Catechol + CO2
3,4-Dihydroxybenzoate
show the reaction diagram
-
-
-
-
additional information
?
-
-
catalyzes a CO2:4-hydroxybenzoate isotope exchange reaction
-
-
-
additional information
?
-
-
no substrates are 2-hydroxybenzoate, 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,3,4-trihydroxybenzoate, 3,4,5-trihydroxybenzoate
-
?
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
-
additional information
?
-
-
induced by 4-hydroxybenzoate
-
-
-
additional information
?
-
-
benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates
-
-
-
additional information
?
-
-, C0H3U9
no reaction with: 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,6-dihydroxybenzoate, and 3,4-dihydroxybenzoate, 2,3,4-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, 3,5-dihydroxy-2-naphthalene dicarboxylate, 2,8-dihydroxynaphthoate, 5-hydroxyisophthalate, 1-hydroxy-2-naphthoate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, 6-hydroxynicotinate, 2-hydroxypyridine-3-carboxylate, 3-hydroxypyridine-2-carboxylate
-
-
-
additional information
?
-
Enterobacter cloacae P240
-
benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates
-
-
-
additional information
?
-
Pseudomonas sp. K172
-
induced after anaerobic growth on phenol
-
-
-
additional information
?
-
Pseudomonas sp. K172
-
catalyzes a CO2:4-hydroxybenzoate isotope exchange reaction
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Phenol + CO2
?
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. K172
-
first step in the anaerobic metabolism of phenol
-
-
-
additional information
?
-
-
induced after anaerobic growth on phenol
-
-
-
additional information
?
-
-
induced by 4-hydroxybenzoate
-
-
-
additional information
?
-
Pseudomonas sp. K172
-
induced after anaerobic growth on phenol
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
Q4R101 and Q9S4M7 and Q4R102
not dependent on biotin, ATP, thiamin or pyridoxal phosphate
-
additional information
-
the activity of 4-hydroxybenzoate decarboxylase is independent of pyridoxal 5'-phosphate, thiamine diphosphate, and the pyruvoyl group
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
1 mM, 1.44fold stimulation of activity
CaCl2
-
2.5 mM, 99% stimulation of activity
CdCl2
-
2.5 mM, 51% stimulation of activity
Co2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Fe2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Fe2+
-
1 mM, 2.52fold stimulation of activity
K+
-
phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on
K+
-
not required for phenylphosphate decarboxylase activity
K+
-
required for CO2:4-hydroxybenzoate isotope exchange reaction
Mg2+
-
can replace to some extent for Mn2+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Mg2+
-
1 mM, 2.99fold stimulation of activity
MgSO4
-
2.5 mM, 125% stimulation of activity
Mn2+
-
minor stimulation of carboxylation of 4-hydroxybenzoate
Mn2+
-
restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8
Mn2+
-
required for phenylphosphate decarboxylase activity
Mn2+
-
required for CO2:4-hydroxybenzoate isotope exchange reaction
Mn2+
-
1 mM, 2.43fold stimulation of activity
Na+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
NH4+
-
stimulates activity of the enzyme form with pH-optimum pH 7.8
Rb+
-
can replace K+ in the phenol carboxylase and partially in both decarboxylases
Rb+
-
can replace to some extent for K+ in the CO2:4-hydroxybenzoate isotope exchange reaction
Zn2+
-
compensates the loss of activity by EDTA
ZnCl2
-
2.5 mM, 75% stimulation of activity
ZnSO4
-
2.5 mM, 100% stimulation of activity
MnCl2
-
2.5 mM, 112% stimulation of activity
additional information
Q4R101 and Q9S4M7 and Q4R102
no metal ions
additional information
-
AgNO3, FeCl2, CoCl2, NiCl2, and FeSO4 do not affect activity
additional information
-
not stimulated by Cu2+ or Zn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
-
-
2,3,4-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4,6-Trihydroxybenzoate
-
-
2,4,6-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4-Dihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
slight inhibition of carboxylation of phenol
3-Chloro-4-hydroxybenzoate
-
-
CuCl2
-
2.5 mM, 91% loss of activity
CuSO4
-
2.5 mM, 92% loss of activity
EDTA
-
47% inhibition at 5 mM, Mg2+, Mn2+, Zn2+ or Ca2+ protect, Fe2+ does not
EDTA
-
2.5 mM, 22% inhibition
HgCl2
-
2.5 mM, 78% loss of activity
NH4+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Rb+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Zn2+
-
destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate
m-Hydroxybenzoylhydrazine dichloride
-
-
additional information
-
not significantly affected by phenylhydrazine or hydroxylamine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no stimulation by ATP up to 5 mM or phenylphosphate
-
additional information
-
1 mM pyridoxal 5'-phosphate or thiamine diphosphate do not enhance activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.2
-
3,4-dihydroxybenzoate
-
-
6.8
-
3,4-dihydroxybenzoate
-
in 100 mM potassium phosphate buffer (pH 7.0)
0.004
-
4-hydroxybenzoate
-
carboxylase with pH-optimum 7.8
0.02
-
4-hydroxybenzoate
-
carboxylase with pH-optimum 6.4
0.21
-
4-hydroxybenzoate
-
in 20 mM Tris-HCl (pH 7.5) containing 1 mM Mg2+, at 30C
0.4
-
4-hydroxybenzoate
-
-
0.596
-
4-hydroxybenzoate
-
in 100 mM potassium phosphate buffer (pH 7.0)
0.67
-
4-hydroxybenzoate
-
-
4
-
4-hydroxybenzoate
-
-
5.4
-
4-hydroxybenzoate
-
pH 6.0, 37C
1
-
CO2
-
dissolved, CO2:4-hydroxybenzoate isotope exchange reaction
14.8
-
Phenol
-
20 mM phenol, 3 MKHCO3, 10 mM dithiothreitol in 100 mM potassium phosphate buffer (pH 7.0)
0.2
-
Phenylphosphate
-
CO2:4-hydroxybenzoate isotope exchange reaction
1.5
-
CO2
-
dissolved
additional information
-
additional information
-, C0H3U9
4-hydroxybenzoate as substrate Km = 1 mmol/l; vanillate as substrate Km = 4 mmol/l
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
18.3
-
3,4-dihydroxybenzoate
-
-
55
-
4-hydroxybenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00025
-
-
crude cell extract, at 30C
0.00506
-
-
after purification, at 30C
0.012
-
-
phenylphosphate carboxylation
2.5
-
-, C0H3U9
uninduced, 4-hydroxybenzoate as substrate
6
-
-, C0H3U9
3-methoxy-4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate
11
-
-, C0H3U9
BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 3-methoxy-4-hydroxybenzoate as substrate
13.7
-
-
at 30C, pH 7.0, crude cell extract
14
-
-, C0H3U9
BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 4-hydroxybenzoate as substrate
15
-
-, C0H3U9
uninduced, 3-methoxy-4-hydroxybenzoate as substrate
16
-
-, C0H3U9
BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 3-methoxy-4-hydroxybenzoate as substrate
17.8
-
-
at 30C, pH 7.0, after 1.5fold purification
23
-
-, C0H3U9
BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 4-hydroxybenzoate as substrate
25
-
-, C0H3U9
3-methoxy-4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate
30
-
-, C0H3U9
4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate
31
-
-, C0H3U9
4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
phenol carboxylation
6.5
-
-
phenylphosphate carboxylation
6.5
-
-
in potassium phosphate buffer
7
-
-, C0H3U9
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
in potassium phosphate buffer
37
-
-
enzyme with pH-optimum of pH 7.8
41
-
-
enzyme with pH-optimum of pH 6.4
45
-
-, C0H3U9
assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
5.9
-
isoeolctric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
induced in growing and resting cells by 4-hydroxybenzoate
Manually annotated by BRENDA team
Enterobacter cloacae P240
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8569
-
-, C0H3U9
bsdD, calculated
64000
-
-
SDS-PAGE
280000
-
-
gel filtration
315800
-
-
calculated from amino acid sequence
350000
-
-
gradient PAGE, gel filtration
372000
-
-
gel-filtration HPLC
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexamer
-
6 * 57000, SDS-PAGE
homohexamer
-
6 * 60000, X-ray crystallography
homohexamer
Enterobacter cloacae P240
-
6 * 60000, X-ray crystallography
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7.3
-
stable for 2 h
6
-
-
11-40C, stable for more than 24 h
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
11
40
-
pH 6.0, stable for more than 24 h
20
-
-
when the enzyme is incubated at temperatures up to 20C for 30 min, activity is barely decreased
30
60
-
treatment at 30, 40, 50, and 60C causes 32, 43, 96, and 100% losses of the initial activity, respectively
40
50
-
loses more than 50% decarboxylase activity at 40C for 5 min, and less than 25% enzyme activity remains after incubation at 50C for 5 min
50
-
-
half-life: 12 h
56
-
-
half-life: 15 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
oxygen-sensitive
Q4R101 and Q9S4M7 and Q4R102
extremely sensitive to oxygen, in contact with air, loss of activity within a few hours
-
oxygen sensitive, loss of 50% of activity after 1 h in presence of air at 4C compared to no activity lost after 7 days under anaerobic conditions at 4C
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
half-life in air-saturated solution: less than 1 min
-
4742
oxygen-sensitive, 50% loss of activity when exposed to air at 5C for 2 h
-
4740
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
under anaerobic conditions (oxygen-free N2) at 4C by affinity chromatography
-
DEAE-Sephacel column chromatography, phenyl-Sepharose CL-4B column chromatography, and butyl-Toyopearl 650M column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-, C0H3U9
expressed in Escherichia coli strain BL21 (DE3)
-
-
Q4R101 and Q9S4M7 and Q4R102
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
use of enzyme for conversion of phenol into 4-hydroxybenzoic acid