Information on EC 4.1.1.61 - 4-Hydroxybenzoate decarboxylase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.1.1.61
-
RECOMMENDED NAME
GeneOntology No.
4-Hydroxybenzoate decarboxylase
PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzoate carboxy-lyase (phenol-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4-hydroxybenzoate DC
C0H3U9
-
4-hydroxybenzoate decarboxylase
C0H3U9
-
Decarboxylase, p-hydroxybenzoate
-
-
-
-
HBDC
Chlamydia pneumoniae AR39
-
-
-
p-Hydroxybenzoate decarboxylase
-
-
-
-
Phenol carboxylase
-
-
-
-
vanillate/4-hydroxybenzoate decarboxylase
C0H3U9
-
CAS REGISTRY NUMBER
COMMENTARY
37290-53-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ATCC 6051
UniProt
Manually annotated by BRENDA team
strain AR39
-
-
Manually annotated by BRENDA team
Chlamydia pneumoniae AR39
strain AR39
-
-
Manually annotated by BRENDA team
in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
Manually annotated by BRENDA team
Aerobacter aerogenes
-
-
Manually annotated by BRENDA team
strain P240
-
-
Manually annotated by BRENDA team
Enterobacter cloacae P240
strain P240
-
-
Manually annotated by BRENDA team
in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
-
Manually annotated by BRENDA team
strain K172
-
-
Manually annotated by BRENDA team
Pseudomonas sp. K172
strain K172
-
-
Manually annotated by BRENDA team
-
Q4R101 and Q9S4M7 and Q4R102
UniProt
Manually annotated by BRENDA team
Clostridium hydroxybenzoicum, strain JW/Z-1T
-
-
Manually annotated by BRENDA team
Clostridium-like strain 6
-
-
Manually annotated by BRENDA team
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
Q4R101 and Q9S4M7 and Q4R102
not dependent on biotin, ATP, thiamin or pyridoxal phosphate
-
additional information
-
the activity of 4-hydroxybenzoate decarboxylase is independent of pyridoxal 5'-phosphate, thiamine diphosphate, and the pyruvoyl group
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
-
-
2,3,4-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4,6-Trihydroxybenzoate
-
-
2,4,6-Trihydroxybenzoate
-
complete inhibition of carboxylation of phenol
2,4-Dihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
-
3,4,5-Trihydroxybenzoate
-
slight inhibition of carboxylation of phenol
3-Chloro-4-hydroxybenzoate
-
-
CuCl2
-
2.5 mM, 91% loss of activity
CuSO4
-
2.5 mM, 92% loss of activity
EDTA
-
47% inhibition at 5 mM, Mg2+, Mn2+, Zn2+ or Ca2+ protect, Fe2+ does not
EDTA
-
2.5 mM, 22% inhibition
HgCl2
-
2.5 mM, 78% loss of activity
NH4+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Rb+
-
decreases carboxylation of 3,4-dihydroxybenzoate by about 20%
Zn2+
-
destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate
m-Hydroxybenzoylhydrazine dichloride
-
-
additional information
-
not significantly affected by phenylhydrazine or hydroxylamine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no stimulation by ATP up to 5 mM or phenylphosphate
-
additional information
-
1 mM pyridoxal 5'-phosphate or thiamine diphosphate do not enhance activity
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
18.3
-
3,4-dihydroxybenzoate
-
-
55
-
4-hydroxybenzoate
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
5.9
-
isoeolctric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
induced in growing and resting cells by 4-hydroxybenzoate
Manually annotated by BRENDA team
Enterobacter cloacae P240
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexamer
-
6 * 57000, SDS-PAGE
homohexamer
-
6 * 60000, X-ray crystallography
homohexamer
Enterobacter cloacae P240
-
6 * 60000, X-ray crystallography
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7.3
-
stable for 2 h
6
-
-
11-40C, stable for more than 24 h
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
11
40
-
pH 6.0, stable for more than 24 h
20
-
-
when the enzyme is incubated at temperatures up to 20C for 30 min, activity is barely decreased
30
60
-
treatment at 30, 40, 50, and 60C causes 32, 43, 96, and 100% losses of the initial activity, respectively
40
50
-
loses more than 50% decarboxylase activity at 40C for 5 min, and less than 25% enzyme activity remains after incubation at 50C for 5 min
50
-
-
half-life: 12 h
56
-
-
half-life: 15 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
oxygen-sensitive
Q4R101 and Q9S4M7 and Q4R102
extremely sensitive to oxygen, in contact with air, loss of activity within a few hours
-
oxygen sensitive, loss of 50% of activity after 1 h in presence of air at 4C compared to no activity lost after 7 days under anaerobic conditions at 4C
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
under anaerobic conditions (oxygen-free N2) at 4C by affinity chromatography
-
DEAE-Sephacel column chromatography, phenyl-Sepharose CL-4B column chromatography, and butyl-Toyopearl 650M column chromatography
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
use of enzyme for conversion of phenol into 4-hydroxybenzoic acid