Information on EC 4.1.1.59 - gallate decarboxylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.1.1.59
-
RECOMMENDED NAME
GeneOntology No.
gallate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3,4,5-trihydroxybenzoate = pyrogallol + CO2
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
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-
gallate degradation III (anaerobic)
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
3,4,5-trihydroxybenzoate carboxy-lyase (pyrogallol-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-51-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
strain 64-1
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-
Manually annotated by BRENDA team
strain 64-1
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Manually annotated by BRENDA team
i.e. Aerobacter aerogenes
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-
Manually annotated by BRENDA team
no activity in Erwinia rhapontici
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-
-
Manually annotated by BRENDA team
no activity in Klebsiella terrigena
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-
-
Manually annotated by BRENDA team
no activity in Pantoea agglomerans
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-
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Manually annotated by BRENDA team
i.e. Enterobacter agglomerans, strain T71
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-
Manually annotated by BRENDA team
2 strains, YL-1 and ML-1, isolated from the phyllosphere of green leaves of Rosa rugosa
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-
Manually annotated by BRENDA team
type strain 2.2, formerly Streptococcus caprinus
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4,5-Trihydroxybenzoate
?
show the reaction diagram
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
show the reaction diagram
gallic acid
?
show the reaction diagram
-
-
-
-
?
gallic acid
pyrogallol + CO2
show the reaction diagram
-
-
-
-
?
protocatechuic acid
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4,5-Trihydroxybenzoate
?
show the reaction diagram
3,4,5-Trihydroxybenzoate
Pyrogallol + CO2
show the reaction diagram
gallic acid
pyrogallol + CO2
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
required as cofactor, contains 0.8 mol of iron per mol of enzyme subunit
Mg2+
-
dependent on, Km: 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2S2O8
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-
1,10-phenanthroline
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2,2'-bipyridyl
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Cuprizone
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FeCl2
-
slight
FeCl3
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slight
p-chloromercuribenzoate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2S2O8
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there is an activity increase of 52.7% in the presence of (NH4)2S2O8
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.96 - 3.3
3,4,5-Trihydroxybenzoate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
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pH 5.0: about 55% of maximal activity, pH 7.0: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
-
6 * 57000, SDS-PAGE
320000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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6 * 57000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
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stable in the range
4733
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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stable below
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
crude enzyme is very unstable, several attempts to purify the enzyme have so far been unsuccessful
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dilution and dialysis completely inactivates
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive enzyme
-
4733
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM potassium phosphate buffer, pH 6.0, containing glycerol and reducing agents, stable for 3 weeks
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5°C, 90% loss of activity after 2 days, crude enzyme
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon Superflow resin column chromatography, and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain JW2308-4
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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synthesis of pyrogallol from readily available tannic acid by using the combined action of gallate decarboxylase and tannase in a two-enzyme resting cell bioconversion