Information on EC 4.1.1.57 - methionine decarboxylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.1.1.57
-
RECOMMENDED NAME
GeneOntology No.
methionine decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-methionine = 3-methylthiopropanamine + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-methionine carboxy-lyase (3-methylthiopropanamine-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37290-50-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K37
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DL-Homocysteine
3-Thiopropanamine + CO2
show the reaction diagram
-
24% of the activity with L-Met
-
-
-
L-Ethionine
3-Ethylthiopropanamine + CO2
show the reaction diagram
L-Ile
2-Methylbutylamine + CO2
show the reaction diagram
L-Leu
3-Methylbutylamine + CO2
show the reaction diagram
L-Met
3-Methylthiopropanamine + CO2
show the reaction diagram
L-Met
?
show the reaction diagram
-
induced by L-Met
-
-
-
L-methionine
3-methylthiopropanamine + CO2
show the reaction diagram
-
-
-
?
L-Norleucine
Pentanamine + CO2
show the reaction diagram
L-Norvaline
Butylamine + CO2
show the reaction diagram
L-Val
2-Methylpropylamine + CO2
show the reaction diagram
S-Ethyl-L-Cys
2-Ethylthioethanamine + CO2
show the reaction diagram
S-Methyl-L-Cys
2-Methylthioethanamine + CO2
show the reaction diagram
-
13% of the activity with L-Met
-
-
-
S-Methyl-L-Met
3-Dimethylthiopropanamine + CO2
show the reaction diagram
-
13% of the activity with L-Met
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Met
?
show the reaction diagram
-
induced by L-Met
-
-
-
L-methionine
3-methylthiopropanamine + CO2
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2SO4
-
-
carboxymethoxylamine hemihydrochloride
-
1 mM, 38.9% activity
CuSO4
cycloserine
-
-
cysteine
-
-
D-Met
FeSO4
hydrazine
-
-
hydroxylamine
Isonicotinic acid hydrazide
-
-
MnCl2
-
slight
Penicillamine
-
-
phenylhydrazine
Semicarbazide
-
-
Semicarbazide hydrochloride
-
1 mM, 21.6% activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergent
-
required
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
153
L-Ile
-
-
124
L-Leu
-
-
0.0121 - 48
L-Met
16 - 155
L-norleucine
147
L-norvaline
-
-
1100
L-Val
-
-
44 - 1000
S-ethyl-L-Cys
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50 - 120
L-Met
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.021
-
crude extract
4.45
-
after sequential DEAE-Cellulose, Resource-Q, Mono-Q and Superdex 200 chromatography
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 8.5
-
pH 4.2: about 35% of maximal activity, pH 8.5: about 45% of maximal activity
5 - 9
-
pH 5.0: about 20% of maximal activity, pH 9.0: about 40% of maximal activity
6.5 - 8
-
potassium phosphate buffer, values between 66% and 85% activity with respect to optimum pH
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 45
-
retains approximately 70% activity in this range
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
frond
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the enzyme is not membrane bound, because its activity can be solubilized into the 50 mM potassium phosphate buffer pH 7.2 without detergents
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
-
2 * 57000, SDS-PAGE
59000
-
2 * 59000, SDS-PAGE
100000
130000
-
gel filtration
204000
-
gel filtration chromatography
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 100000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6
-
stable in the range
4721
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
30 min, stable below
40
-
30 min, 20% loss of activity
45
-
pH 7.2, stable up to
additional information
-
pyridoxal 5'-phosphate protects against heat inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
little effect for stabilization to add pyridoxal 5'-phosphate, glycerol or 2-mercaptoethanol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
four purification steps including anion-exchange chromatography and size-exclusion chromatography
-