Information on EC 4.1.1.55 - 4,5-dihydroxyphthalate decarboxylase

Word Map on EC 4.1.1.55
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.1.55
-
RECOMMENDED NAME
GeneOntology No.
4,5-dihydroxyphthalate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4,5-dihydroxyphthalate = 3,4-dihydroxybenzoate + CO2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
Polycyclic aromatic hydrocarbon degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
4,5-dihydroxyphthalate carboxy-lyase (3,4-dihydroxybenzoate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37290-48-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NH1000
-
-
Manually annotated by BRENDA team
no activity in Bacillus sp.
strain FO
-
-
Manually annotated by BRENDA team
no activity in Bacillus sp. FO
strain FO
-
-
Manually annotated by BRENDA team
strain PHK
-
-
Manually annotated by BRENDA team
strain PHK
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain F274
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
show the reaction diagram
4,5-Dihydroxyphthalate
?
show the reaction diagram
4-Hydroxyphthalate
3-Hydroxybenzoate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
show the reaction diagram
4,5-Dihydroxyphthalate
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,4,6-Trinitrobenzene sulfonate
-
inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM
3-hydroxybenzoate
-
-
3-methylcatechol
-
weak
4-hydroxybenzoate
-
-
5,5'-dithiobis(2-nitrobenzoate)
anthranilate
-
competitive
ATP
-
slight
CoA
-
slight
gentisate
-
weak
protocatechuate
-
-
salicylate
-
-
terephthalate
-
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0104 - 0.0105
4,5-Dihydroxyphthalate
1.25
4-Hydroxyphthalate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.7
-
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
4 * 38000, SDS-PAGE
66000
-
6 * 66000, SDS-PAGE
150000
-
gel filtration
420000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
27°C, 3.5 h, about 20% loss of activity
4714
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
pH 7.5, 3.5 h, about 20% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against distilled water or phosphate buffer, 0.067 M, pH 5.9-8.5, at 0-3°C, results in no appreciable loss of activity. Dialysis for 3-4 days causes about 70% loss of activity
-
no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-