Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase

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The expected taxonomic range for this enzyme is: leotiomyceta

EC NUMBER
COMMENTARY
4.1.1.52
-
RECOMMENDED NAME
GeneOntology No.
6-methylsalicylate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
patulin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
-
-
-
-
6-Methylsalicylic acid decarboxylase
-
-
-
-
6-MSA decarboxylase
-
-
-
-
Decarboxylase, 6-methylsalicylate
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37289-50-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aspergillus clavatus NRRL 1980
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
show the reaction diagram
-
at 6% the activity with 6-methylsalicylic acid
-
-
-
6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
show the reaction diagram
-
at 100% of the activity with 6-methylsalicylic acid
-
-
-
6-Methylsalicylate
?
show the reaction diagram
-
enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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-
-
6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
Aspergillus clavatus, Aspergillus clavatus NRRL 1980
T1PRE6
-
-
-
?
6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
-
-
-
-
6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
-
-
-
-
Homoorsellinic acid
?
show the reaction diagram
-
at 100% of the activity with 6-methylsalicylic acid
-
-
-
Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
show the reaction diagram
-
at 80% of the activity with 6-methylsalicylic acid
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-Methylsalicylate
?
show the reaction diagram
-
enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
-
-
-
6-methylsalicylate
3-methylphenol + CO2
show the reaction diagram
Aspergillus clavatus, Aspergillus clavatus NRRL 1980
T1PRE6
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-Hydroxy-1-naphthoic acid
-
-
2-Methylbenzoic acid
-
-
3-methylsalicylic acid
-
-
4-methylsalicylic acid
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-
6-Methylanthranilic acid
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-
6-Methylsalicylic acid
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-
diethyl dicarbonate
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10 mM, complete inhibition
NaNO3
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10 mM, 20% inhibition
Rose bengal
-
photooxidation
salicylic acid
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.15
3-bromoorsellinic acid
-
-
0.01
6-Ethylsalicylic acid
-
6-methylsalicylic acid
0.007
6-Methylsalicylic acid
-
-
0.04
Homoorsellinic acid
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-
0.1
orsellinic acid
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
56000 - 60000
-
gel filtration, sucrose density gradient sedimentation
4703
70000
-
gel filtration
4701
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
T1PRE6
x * 43000, V5 His-tagged enzyme, SDS-PAGE
?
Aspergillus clavatus NRRL 1980
-
x * 43000, V5 His-tagged enzyme, SDS-PAGE
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
unstable below
4701
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
-
5 min, unstable
4701
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in solutions of low ionic strength
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
-
4703
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
-
-25°C, pH 7.5, without glycerol, stable for at least 1 year
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
T1PRE6
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
T1PRE6
when Aspergillus clavatus is grown under restrictive conditions (MEM) which does not support patulin production, the expression of the enzyme is inhibited
Aspergillus clavatus NRRL 1980
-
-
the enzyme is strongly expressed during patulin production whereas it is very weakly expressed in non-patulin permissive conditions
Aspergillus clavatus NRRL 1980
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-