Information on EC 4.1.1.52 - 6-methylsalicylate decarboxylase

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The expected taxonomic range for this enzyme is: leotiomyceta

EC NUMBER
COMMENTARY
4.1.1.52
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RECOMMENDED NAME
GeneOntology No.
6-methylsalicylate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
6-methylsalicylate = 3-methylphenol + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
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-
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SYSTEMATIC NAME
IUBMB Comments
6-methylsalicylate carboxy-lyase (3-methylphenol-forming)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase
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6-Methylsalicylic acid decarboxylase
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6-MSA decarboxylase
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Decarboxylase, 6-methylsalicylate
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CAS REGISTRY NUMBER
COMMENTARY
37289-50-2
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Bromoorsellinic acid
1,3-Dihydroxy-2-bromo-5-methylbenzene + CO2
show the reaction diagram
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at 6% the activity with 6-methylsalicylic acid
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6-Ethylsalicylic acid
3-Ethyl-1-hydroxybenzene + CO2
show the reaction diagram
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at 100% of the activity with 6-methylsalicylic acid
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6-Methylsalicylate
?
show the reaction diagram
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enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
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6-Methylsalicylic acid
3-Cresol + CO2
show the reaction diagram
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Homoorsellinic acid
?
show the reaction diagram
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at 100% of the activity with 6-methylsalicylic acid
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Orsellinic acid
1,3-Dihydroxy-5-methylbenzene + CO2
show the reaction diagram
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at 80% of the activity with 6-methylsalicylic acid
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-Methylsalicylate
?
show the reaction diagram
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enzyme catalyzes the first step in the conversion of 6-methylsalicylic acid into patulin
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INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-Hydroxy-1-naphthoic acid
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2-Methylbenzoic acid
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3-methylsalicylic acid
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4-methylsalicylic acid
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6-Methylanthranilic acid
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6-Methylsalicylic acid
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diethyl dicarbonate
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10 mM, complete inhibition
NaNO3
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10 mM, 20% inhibition
Rose bengal
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photooxidation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.15
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3-bromoorsellinic acid
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0.01
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6-Ethylsalicylic acid
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6-methylsalicylic acid
0.007
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6-Methylsalicylic acid
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0.04
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Homoorsellinic acid
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0.1
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orsellinic acid
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
grown in Czapek medium containing cycloheximide, no activity in rich germinating medium
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
56000
60000
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gel filtration, sucrose density gradient sedimentation
70000
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gel filtration
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
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unstable below
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
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5 min, unstable
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable in solutions of low ionic strength
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OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
photooxidation in presence of rose Bengal is prevented in presence of substrate or 2-hydroxy-6-methylsalicylic acid
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4703
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-15°C, pH 7.5, 50% glycerol, 5% w/v (NH4)2SO4, stable for several months
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-25°C, pH 7.5, without glycerol, stable for at least 1 year
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