Information on EC 4.1.1.51 - 3-Hydroxy-2-methylpyridine-4,5-dicarboxylate 4-decarboxylase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.1.1.51
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RECOMMENDED NAME
GeneOntology No.
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate 4-decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3-hydroxy-2-methylpyridine-4,5-dicarboxylate = 3-hydroxy-2-methylpyridine-5-carboxylate + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
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-
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PATHWAY
KEGG Link
MetaCyc Link
vitamin B6 degradation
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Vitamin B6 metabolism
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxy-2-methylpyridine-4,5-dicarboxylate 4-carboxy-lyase (3-hydroxy-2-methylpyridine-5-carboxylate-forming)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase
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Decarboxylase, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate 4-
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HMPDdc
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the enzyme is involved in the catabolism of pyridoxal 5'-phosphate
CAS REGISTRY NUMBER
COMMENTARY
37289-49-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain MAFF303099
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-
Manually annotated by BRENDA team
strain MA-1
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Manually annotated by BRENDA team
strain MA-1
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
show the reaction diagram
-
-
-
-
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
show the reaction diagram
-
-
-
-
?
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
show the reaction diagram
-
-
-
-
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
?
show the reaction diagram
-
inducible enzyme. Essential reaction in the degradative utilization of vitamin B6 as an energy and carbon source
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
?
show the reaction diagram
-
inducible enzyme. Essential reaction in the degradative utilization of vitamin B6 as an energy and carbon source
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mn2+
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required
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Reducing agent
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e.g. cyanide or SH-reagents, required
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.036
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3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
-
-
0.366
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3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
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in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.6
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3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
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in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.8
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pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8.9
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pH 6.5: about 40% of maximal activity, pH 8.9: about 60% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
as principal source of carbon and nitrogen
Manually annotated by BRENDA team
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as principal source of carbon and nitrogen
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Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Rhizobium loti (strain MAFF303099)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
26000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
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x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging drop vapour diffusion method, optimized crystallization conditions consist of 6-9% polyethylene glycol 8000 and 100 mM Tris buffer with a pH ranging from 7.0 to 7.5
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli strain BL21(DE3)
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