Information on EC 4.1.1.46 - o-Pyrocatechuate decarboxylase

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The expected taxonomic range for this enzyme is: Dikarya

EC NUMBER
COMMENTARY
4.1.1.46
-
RECOMMENDED NAME
GeneOntology No.
o-Pyrocatechuate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2,3-dihydroxybenzoate = catechol + CO2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
Benzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxybenzoate carboxy-lyase (catechol-forming)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2,3-DHBA decarboxylase
-
-
-
-
2,3-Dihydroxybenzoic acid decarboxylase
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-
-
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Decarboxylase, o-pyrocatechuate
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-
-
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DHBA decarboxylase
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-
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DHBD
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-
-
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o-Pyrocatechuic acid carboxy-lyase
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
37289-48-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain UBC814
-
-
Manually annotated by BRENDA team
Aspergillus niger UBC814
strain UBC814
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3,5-Trihydroxybenzoate
1,2,4-Trihydroxybenzene + CO2
show the reaction diagram
-
18% of the activity with 2,3-dihydroxybenzoate
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
-
-
-
-
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
Aspergillus niger UBC814
-
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
induced to over 6times its basal level by the addition of 0.1% Trp
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the pathway for the biodegradation of indole, enzyme is induced by indole
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the catabolism of Trp, induced by both L-Trp and salicylate
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the biosynthesis of catechol
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
induced to over 6times its basal level by the addition of 0.1% Trp
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the pathway for the biodegradation of indole, enzyme is induced by indole
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the catabolism of Trp, induced by both L-Trp and salicylate
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
-
enzyme is involved in the biosynthesis of catechol
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
the enzyme does not contain a prosthetic group
-
additional information
-
-
-
additional information
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-
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INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
-
-
2,3,4-Trihydroxybenzoate
-
-
2,3-Dihydroxybenzaldehyde
-
-
2,3-Dimercaptopropanol
-
-
2,4-Dihydroxybenzoate
-
-
2,4-Dihydroxybenzoate
-
competitive
2,4-Dihydroxybenzoate
-
-
2,5-Dihydroxybenzoate
-
-
2,6-Dihydroxybenzoate
-
-
2-Hydroxybenzoate
-
-
3,4-dihydroxybenzoate
-
weak
3,4-dihydroxybenzoate
-
weak
3-Hydroxyanthranilate
-
-
3-Hydroxybenzoate
-
weak
3-Hydroxybenzoate
-
-
anthranilate
-
-
Benzoate
-
weak
Benzoate
-
weak
catechol
-
competitive
diethyldicarbonate
-
substrate analogues protect
diethyldicarbonate
-
-
Fe2+
-
partial
-
Fe3+
-
partial
-
iodoacetate
-
-
N-bromosuccinimide
-
-
NaBH4
-
irreversible
NEM
-
prevented in presence of salicylate
o-phenanthroline
-
1 mM, 50% inactivation
reduced glutathione
-
-
salicylate
-
competitive
Mercapotoethanol
-
weak
additional information
-
no inhibition by 4-hydroxybenzoate
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.037
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2,3-Dihydroxybenzoate
-
-
0.3
-
2,3-Dihydroxybenzoate
-
-
0.34
-
2,3-Dihydroxybenzoate
-
-
0.42
-
2,3-Dihydroxybenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
15.9
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
7
-
pH 4.5: about 35% of maximal activity, pH 7.0: about 30% of maximal activity
6
9.7
-
about 50% of maximal activity at pH 6.0 and at pH 9.7
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
with increasing mycelial age, the basal enzyme activity level remains relatively unchanged, while the level to which the enzyme could be induced decreases significantly
Manually annotated by BRENDA team
Aspergillus niger UBC814
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
66100
-
-
gel filtration
120000
-
-
gel filtration
130000
-
-
gel filtration
150000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 38000, SDS-PAGE
dimer
-
2 * 36500, SDS-PAGE
tetramer
-
4 * 28000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, stable for about 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE