Information on EC 4.1.1.46 - o-Pyrocatechuate decarboxylase

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The expected taxonomic range for this enzyme is: Dikarya

EC NUMBER
COMMENTARY hide
4.1.1.46
-
RECOMMENDED NAME
GeneOntology No.
o-Pyrocatechuate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,3-dihydroxybenzoate = catechol + CO2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
-
Benzoate degradation
-
-
Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxybenzoate carboxy-lyase (catechol-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-48-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain UBC814
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3,5-Trihydroxybenzoate
1,2,4-Trihydroxybenzene + CO2
show the reaction diagram
-
18% of the activity with 2,3-dihydroxybenzoate
-
-
-
2,3-Dihydroxybenzoate
?
show the reaction diagram
2,3-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-Dihydroxybenzoate
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
2,3-Dihydroxybenzaldehyde
-
-
2,3-Dimercaptopropanol
-
-
2,4-Dihydroxybenzoate
2,5-Dihydroxybenzoate
-
-
2,6-Dihydroxybenzoate
-
-
2-Hydroxybenzoate
-
-
3,4-dihydroxybenzoate
3-Hydroxyanthranilate
-
-
3-hydroxybenzoate
anthranilate
-
-
Benzoate
catechol
-
competitive
Cd2+
-
partial
Cu2+
-
partial
cysteine
-
-
diethyldicarbonate
Fe2+
-
partial
Fe3+
-
partial
iodoacetate
-
-
Mercapotoethanol
-
weak
N-bromosuccinimide
-
-
NaBH4
-
irreversible
o-phenanthroline
-
1 mM, 50% inactivation
reduced glutathione
-
-
salicylate
additional information
-
no inhibition by 4-hydroxybenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037 - 0.42
2,3-Dihydroxybenzoate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7
-
pH 4.5: about 35% of maximal activity, pH 7.0: about 30% of maximal activity
6 - 9.7
-
about 50% of maximal activity at pH 6.0 and at pH 9.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66100
-
gel filtration
120000
-
gel filtration
130000
-
gel filtration
150000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 38000, SDS-PAGE
dimer
-
2 * 36500, SDS-PAGE
tetramer
-
4 * 28000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for about 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE