Information on EC 4.1.1.44 - 4-carboxymuconolactone decarboxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.1.1.44
-
RECOMMENDED NAME
GeneOntology No.
4-carboxymuconolactone decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 4,5-dihydro-5-oxofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 4,5-dihydro-5-oxofuran-2-acetate + CO2
show the reaction diagram
syn-reaction
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Benzoate degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
protocatechuate degradation II (ortho-cleavage pathway)
-
SYSTEMATIC NAME
IUBMB Comments
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate carboxy-lyase (4,5-dihydro-5-oxofuran-2-acetate-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase
-
bifunctional enzyme
3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase
Rhodococcus sp. RHA1
-
bifunctional enzyme
-
CMD
-
-
-
-
gamma-carboxymuconolactone decarboxylase
-
-
-
-
gamma-carboxymuconolactone decarboxylase
Q6SJC5
-
gamma-carboxymuconolactone decarboxylase
Q6SJB9
-
gamma-carboxymuconolactone decarboxylase
Q9L5B1
-
gamma-carboxymuconolactone decarboxylase
-
-
gamma-carboxymuconolactone decarboxylase
Sulfitobacter pontiacus ChLG 10
-
-
-
gamma-carboxymuconolactone decarboxylase
Sulfitobacter sp.
Q6SJA7
-
gamma-carboxymuconolactone decarboxylase
-
-
PcaC
Q6SJB9
-
PcaC
Sulfitobacter pontiacus ChLG 10
-
-
-
PcaC
Sulfitobacter sp.
Q6SJA7
-
PcaL
Rhodococcus sp. RHA1
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37289-46-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 1CP
-
-
Manually annotated by BRENDA team
Rhodococcus opacus 1CP
strain 1CP
-
-
Manually annotated by BRENDA team
strain RHA1
-
-
Manually annotated by BRENDA team
Rhodococcus sp. RHA1
strain RHA1
-
-
Manually annotated by BRENDA team
Sulfitobacter pontiacus ChLG 10
-
-
-
Manually annotated by BRENDA team
Sulfitobacter sp.
strain EE-36, Y3F, and Y4I
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
-
beta-ketoadipate enol-lactone
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
-
(4R,5R)-5-[2H]-4-carboxymuconolactone
(2R,5R)-2,5-di[2H]enol-lactone
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
Rhodococcus opacus 1CP
-
-
-
-
?
4-carboxymuconolactone
?
show the reaction diagram
-
inducible enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
inducible enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
constitutive enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
enzyme of the beta-ketoadipate pathway
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
enzyme of the beta-ketoadipate pathway
-
-
-
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q6SJB9
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q6SJC5
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Sulfitobacter sp.
Q6SJA7
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q9L5B1
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Sulfitobacter pontiacus ChLG 10
-
-
-
-
?
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
-
-
-
-
?
gamma-carboxymuconolactone
beta-ketoadipate enol-lactone + ?
show the reaction diagram
Rhodococcus sp., Rhodococcus sp. RHA1
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
?
show the reaction diagram
-
inducible enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
inducible enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
constitutive enzyme
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
enzyme of the beta-ketoadipate pathway
-
-
-
4-carboxymuconolactone
?
show the reaction diagram
-
enzyme of the beta-ketoadipate pathway
-
-
-
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q6SJB9
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q6SJC5
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Sulfitobacter sp.
Q6SJA7
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Q9L5B1
-
-
-
?
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
-
-
-
-
?
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
Sulfitobacter pontiacus ChLG 10
-
-
-
-
?
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.82
-
4-carboxymuconolactone
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
Q6SJC5
assay at
7.5
-
-
assay at
PDB
SCOP
CATH
ORGANISM
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
15950
-
-
caculated value
93000
-
-
-
additional information
-
-
homologies in the NH2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases, ec 5.3.3.4
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging-drop vapour-diffusion method, 22%(w/v) polyethylene glycol 3350, 0.2 M sodium chloride, 0.1 M Na HEPES pH 7.0 and 10%(v/v) glycerol, the crystal belongs to space group P43212, with unit-cell parameters a = b = 66.66, c = 184.82 A, resolution 2.40 A, the Matthews coefficient and solvent content are estimated to be 2.14 A3/Da and 42.6%, respectively, assuming that the asymmetric unit contains three recombinant protein molecules
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
copurification of 3-oxoadipate enol-lactone-hydrolyzing activity, ec 3.1.1.24 and 4-carboxymuconolactone-decarboxylating activity, ec 4.1.1.44
-
centrifugal ultrafiltration, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expressed in Escherichia coli
Q6SJC5
expressed in Escherichia coli
-
overexpression in Escherichia coli strain Rosetta 2 (DE3)
-