Information on EC 4.1.1.44 - 4-carboxymuconolactone decarboxylase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
4.1.1.44
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RECOMMENDED NAME
GeneOntology No.
4-carboxymuconolactone decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 4,5-dihydro-5-oxofuran-2-acetate + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxymandelate degradation
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Benzoate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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protocatechuate degradation II (ortho-cleavage pathway)
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SYSTEMATIC NAME
IUBMB Comments
(R)-2-carboxy-2,5-dihydro-5-oxofuran-2-acetate carboxy-lyase (4,5-dihydro-5-oxofuran-2-acetate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-46-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
strain 1CP
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Manually annotated by BRENDA team
strain 1CP
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Manually annotated by BRENDA team
strain RHA1
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Manually annotated by BRENDA team
strain RHA1
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Sulfitobacter pontiacus ChLG 10
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-
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Manually annotated by BRENDA team
Sulfitobacter sp.
strain EE-36, Y3F, and Y4I
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran-2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
?
show the reaction diagram
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
-
-
-
-
?
gamma-carboxymuconolactone
beta-ketoadipate enol-lactone + ?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-carboxymuconolactone
5-oxo-4,5-dihydrofuran 2-acetate + CO2
show the reaction diagram
4-carboxymuconolactone
?
show the reaction diagram
4-carboxymuconolactone
beta-oxoadipate enol-lactone + ?
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.82
4-carboxymuconolactone
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15950
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caculated value
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method, 22%(w/v) polyethylene glycol 3350, 0.2 M sodium chloride, 0.1 M Na HEPES pH 7.0 and 10%(v/v) glycerol, the crystal belongs to space group P43212, with unit-cell parameters a = b = 66.66, c = 184.82 A, resolution 2.40 A, the Matthews coefficient and solvent content are estimated to be 2.14 A3/Da and 42.6%, respectively, assuming that the asymmetric unit contains three recombinant protein molecules
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
centrifugal ultrafiltration, gel filtration
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copurification of 3-oxoadipate enol-lactone-hydrolyzing activity, ec 3.1.1.24 and 4-carboxymuconolactone-decarboxylating activity, ec 4.1.1.44
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
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overexpression in Escherichia coli strain Rosetta 2 (DE3)
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