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Information on EC 4.1.1.39 - ribulose-bisphosphate carboxylase and Organism(s) Spinacia oleracea and UniProt Accession P00875

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.39 ribulose-bisphosphate carboxylase
IUBMB Comments
Will utilize O2 instead of CO2, forming 3-phospho-D-glycerate and 2-phosphoglycolate.
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This record set is specific for:
Spinacia oleracea
UNIPROT: P00875
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Word Map
The taxonomic range for the selected organisms is: Spinacia oleracea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase, ribulose-1,5-bisphosphate carboxylase, rubpcase, ribulose bisphosphate carboxylase, ribulose 1,5-bisphosphate carboxylase/oxygenase, ribulose 1,5-bisphosphate carboxylase, ribulose bisphosphate carboxylase/oxygenase, rubp carboxylase, rubisco small subunit, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Carboxydismutase
-
-
-
-
D-ribulose 1,5-bisphosphate carboxylase/oxygenase
-
-
D-Ribulose 1,5-diphosphate carboxylase
-
-
-
-
D-Ribulose-1,5-bisphosphate carboxylase
-
-
-
-
D-ribulose-1,5-bisphosphate carboxylase/oxygenase
-
-
Diphosphoribulose carboxylase
-
-
-
-
LESS17
-
-
-
-
PSS15
-
-
-
-
PSSU1
-
-
-
-
Ribulose 1,5-bisphosphate carboxylase
-
-
-
-
Ribulose 1,5-bisphosphate carboxylase-oxygenase
-
-
-
-
Ribulose 1,5-bisphosphate carboxylase/oxygenase
Ribulose 1,5-diphosphate carboxylase
-
-
-
-
Ribulose 1,5-diphosphate carboxylase/oxygenase
-
-
-
-
Ribulose bisphosphate carboxylase-oxygenase
-
-
-
-
Ribulose diphosphate carboxylase
-
-
-
-
Ribulose diphosphate carboxylase/oxygenase
-
-
-
-
ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase
-
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
ribulose-bisphosphate carboxylase
-
-
Rubisco
RuBP carboxylase
-
-
-
-
Water stress responsive proteins 1, 2 and 14
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylation
oxygenation
SYSTEMATIC NAME
IUBMB Comments
3-phospho-D-glycerate carboxy-lyase (dimerizing; D-ribulose-1,5-bisphosphate-forming)
Will utilize O2 instead of CO2, forming 3-phospho-D-glycerate and 2-phosphoglycolate.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-23-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-ribulose 1,5-bisphosphate + CO2 + H2O
3-phospho-D-glycerate
show the reaction diagram
-
-
-
?
3-phospho-D-glycerate + H+
D-ribulose 1,5-bisphosphate + CO2 + H2O
show the reaction diagram
D-Ribulose 1,5-bisphosphate + CO2
?
show the reaction diagram
-
-
-
-
?
D-Ribulose 1,5-bisphosphate + CO2
D-3-Phosphoglycerate
show the reaction diagram
-
-
-
-
?
D-ribulose 1,5-bisphosphate + CO2 + H2O
3-phospho-D-glycerate
show the reaction diagram
D-ribulose 1,5-bisphosphate + CO2 + H2O
3-phospho-D-glycerate + H+
show the reaction diagram
-
-
-
?
D-ribulose 1,5-bisphosphate + O2
3-phospho-D-glycerate + 2-phosphoglycolate
show the reaction diagram
D-Ribulose 1,5-bisphosphate + O2
D-3-Phosphoglycerate + 2-phosphoglycolate
show the reaction diagram
-
-
-
-
?
D-ribulose 1,5-bisphosphate + O2
pentodiulose-1,5-bisphosphate + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco)
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-phospho-D-glycerate + H+
D-ribulose 1,5-bisphosphate + CO2 + H2O
show the reaction diagram
D-Ribulose 1,5-bisphosphate + CO2
?
show the reaction diagram
-
-
-
-
?
D-ribulose 1,5-bisphosphate + CO2 + H2O
3-phospho-D-glycerate
show the reaction diagram
D-ribulose 1,5-bisphosphate + CO2 + H2O
3-phospho-D-glycerate + H+
show the reaction diagram
-
-
-
?
additional information
?
-
-
Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco)
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
contains Mg2+
Ce3+
-
the carboxylase activity of Rubisco greatly increases under low concentrations of Ce3+
Co2+
-
activation
Cu2+
-
activation
Nd3+
-
activates Rubisco carboxylase activity 2.4fold
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
-
arsenite
-
-
Ce3+
-
the carboxylase activity of Rubisco decreased under high concentrations of Ce3+
D-Glycero-2,3-pentodiulose 1,5-bisphosphate
-
-
D-glycero-2,3-pentodiulose-1,5-bisphosphate
-
potent inhibitor of activity with D-ribulose 1,5-bisphosphate
D-ribulose 1,5-bisphosphate
-
dead-end inhibition
O2
-
competitive inhibition
p-chloromercuribenzoate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CO2
-
activates at high concentrations
NaHCO3
-
20 mM
nano-anatase
-
the activity of Rubisco in the nano-anatase treated spinach is significantly higher than the control, by up to 2.33 times
-
Ribulose-1,5-bisphosphate carboxylase/oxygenase activase
-
characterization of binding to large subunit
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 22
CO2
0.0015 - 0.12
D-ribulose 1,5-bisphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7
CO2
-
at pH 8.3
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.08
-
oxygenase
0.85
-
carboxylase
1.21
-
carboxylase activity, control
1.24
-
carboxylase activity, bulk-TiO2
1.48
-
carboxylase
1.8
-
carboxylase
2.76
-
avtivated by 20 mM NaHCO3, carboxylase
2.83
-
carboxylase activity, nano-anatase, TiO2 treated
additional information
-
oxygenase activity, control 0.00524, bulk-TiO2 0.00519, nano-anatase, TiO2 treated 0.00580
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 8
-
initial reaction phase
7.9
-
carboxylation
8 - 8.2
-
linear reaction phase
9.4
-
oxygenation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
Rubisco from leaves grown at low temperatures
30
-
Rubisco from leaves grown at high temperatures
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ribulose 1,5-bisphosphate carboxylase large chain
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the catalytic incorporation of CO2 into D-ribulose 1,5-bisphosphate by Rubisco is the first step in the production of carbohydrates by plants, which are used to build biomass and produce energy during growth and development
metabolism
the small subunit may function as a reservoir for CO2 storage
physiological function
the catalytic incorporation of CO2 into D-ribulose 1,5-bisphosphate by Rubisco is the first step in the production of carbohydrates by plants, which are used to build biomass and produce energy during growth and development
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RBL_SPIOL
475
0
52740
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
-
1 * 55000 + 1 * 14000, SDS-PAGE
14400
-
Rubisco small subunit, rbcS, determined by SDS-PAGE and Western blot analysis
15000
-
8 * 55000 + 8 * 15000
55000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
1 * 55000 + 1 * 14000, SDS-PAGE
hexadecamer
-
8 * 55000 + 8 * 15000
multimer
catalytic large and small subunit
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complexed with 2-carboxyarabinitol bisphosphate
-
cryoelectron microscopy is used to produce an 11-A density map of the Rubisco-Rubisco large subunit methyltransferase (RLSMT) complex. The atomic model of the complex, obtained by fitting crystal structures of Rubisco and Rubisco large subunit methyltransferase into the density map, shows that the extensive contact regions between the 2 proteins are mainly mediated by hydrophobic residues and leucine-rich repeats
-
crystal structure of an enzyme complex containing EC 4.1.1.39
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 65
-
Rubisco from leaves grown at high temperatures is stable even at 62.5°C. Rubisco from leaves grown at low temperatures decreases its activity above 60°C. The activity of low temperature-Rubisco after the treatment at 65°C is less than that of high temperature-Rubisco by 50%, the Rubisco activation state decreases with increasing temperature above the growth temperature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
when assayed in vitro, the enzyme itself exhibits a slow loss of activity even with saturating levels of all reaction components. This slow inactivation is facilitated by high temperature, low concentration of CO2 or Mg2+ and high O2
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 6-12 months
-
4°C, significant decline of activity in 3 days
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, rate zonal centrifugation using sucrose gradients or Sephacryl S-300 gel filtration (alternatively), and Q Sepharose column chromatography
-
Mono-Q column chromatography
-
polyethylene glycol precipitation and Protein-Pak Q column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the rbcS cDNA is expressed in Chlamydomonas reinhardtii rbcS deletion mutant T60-3 by fusing it to a Chlamydomonas rbcS transit peptide sequence engineered to contain rbcS introns
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
target for increasing agricultural productivity
biotechnology
-
RuBisCO protein readily forms a network with a very high gel strength, but upon deformation it has a brittle character (low critical strain, low fracture strain). RuBisCO exhibits high potential as a functional ingredient for the design of new textures at low protein concentration
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bergenhem, N.; Carlsson, U.
Rapid ion-exchange chromatography for preparative separations of proteins. II. Application to anaerobic purification of ribulose-1,5-bisphosphate carboxylase/oxygenase
J. Chromatogr.
360
279-281
1986
Spinacia oleracea
-
Manually annotated by BRENDA team
Salvucci, M.E.; Portis, A.R.; Orgen, W.L.
Purification of ribulose-1,5-bisphosphate carboxylase/oxygenase large with high specific activity by fast protein liquid chromatography
Anal. Biochem.
153
97-101
1986
Spinacia oleracea
Manually annotated by BRENDA team
Jordan, D.B.; Ogren, W.L.
Species variation in kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase
Arch. Biochem. Biophys.
227
425-433
1983
Amaranthus hybridus, Aphanizomenon flos-aquae, Aphanocapsa alpicola, Echinochloa crus-galli, Glycine max, Helianthus maximus, Medicago sativa, Solanum lycopersicum, Nicotiana tabacum, Panicum milioides, Petroselinum crispum, Plectonema borganum, Polypodium aureum, Portulaca oleracea, Rhodospirillum rubrum, Setaria italica, Sorghum bicolor, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Johal, S.; Bourque, D.P.; Smith, W.W.; Suh, S.W.; Eisenberg, D.
Crystallization and characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from eight plant species
J. Biol. Chem.
255
8873-8880
1980
Gossypium hirsutum, Medicago sativa, Solanum lycopersicum, Nicotiana sylvestris, Nicotiana tabacum, Solanum tuberosum, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Goldthwaite, J.; Bogorad, L.
Ribulose-1,5-diphosphate carboxylase from leaf
Methods Enzymol.
42C
481-484
1975
Citrus sinensis, Glycine max, Gossypium hirsutum, Helianthus annuus, Helianthus maximus, Medicago sativa, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa, Petroselinum crispum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Zea mays
-
Manually annotated by BRENDA team
Siegel, M.I.; Lane, M.D.
Ribulose-diphosphate carboxylase from spinach leaves
Methods Enzymol.
42C
472-489
1975
Spinacia oleracea
-
Manually annotated by BRENDA team
Yokota, A.; Tsujimoto, N.
Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase carrying ribulose 1,5-bisphosphate at its regulatory sites and the mechanism of interaction of this form of the enzyme with ribulose-1,5-bisphosphate-carboxylase/oxygenase activase
Eur. J. Biochem.
204
901-909
1992
Spinacia oleracea
Manually annotated by BRENDA team
Hosur, M.V.; Sainis, J.K.; Kannan, K.K.
Crystallization and x-ray analysis of a multienzyme complex containing RUBISCO and RuBP
J. Mol. Biol.
234
1274-1278
1993
Spinacia oleracea
Manually annotated by BRENDA team
Read, B.A.; Tabita, F.R.
High substrate specificity factor ribulose bisphosphate carboxylase/oxygenase from eukaryotic marine algae and properties of recombinant cyanobacterial rubisco containing algal residue modifications
Arch. Biochem. Biophys.
312
210-218
1994
Cryptomonas sp., Cylindrotheca fusiformis, Cylindrotheca sp., Olisthodiscus luteus, Porphyridium purpureum, Spinacia oleracea, Synechococcus sp., Cylindrotheca sp. N1
Manually annotated by BRENDA team
Kane, H.J.; Wilkin, J.M.; Portis, A.R.; Andrews, T.J.
Potent inhibition of ribulose-bisphosphate carboxylase by an oxidized impurity in ribulose-1,5-bisphosphate
Plant Physiol.
117
1059-1069
1998
Spinacia oleracea
Manually annotated by BRENDA team
Spreitzer, R.J.; Salvucci, M.E.
Rubisco: Structure, regulatory interactions, and possibilities for a better enzyme
Annu. Rev. Plant Biol.
53
449-475
2002
Cupriavidus necator, Anabaena sp., Trichormus variabilis, Synechococcus elongatus PCC 7942 = FACHB-805, Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorobaculum tepidum, Cylindrotheca sp., Flaveria bidentis, Galdieria partita, Helianthus annuus, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa, Thermococcus kodakarensis, Rhodospirillum rubrum, Spinacia oleracea, Synechococcus sp., Zea mays, Amphidinium carterae, Anabaena sp. CA
Manually annotated by BRENDA team
Parry, M.A.; Andralojc, P.J.; Mitchell, R.A.; Madgwick, P.J.; Keys, A.J.
Manipulation of Rubisco: the amount, activity, function and regulation
J. Exp. Bot.
54
1321-1333
2003
Allochromatium vinosum, Arabidopsis thaliana, Avena sativa, Chlamydomonas reinhardtii, Cylindrotheca sp., Flaveria bidentis, Galdieria partita, Galdieria sulphuraria, Glycine max, Helianthus annuus, Hordeum vulgare, Solanum lycopersicum, Nicotiana tabacum, Olisthodiscus luteus, Oryza sativa, Petunia sp., Phaeodactylum tricornutum, Pisum sativum, Rhodobacter capsulatus, Cereibacter sphaeroides, Rhodospirillum rubrum, Spinacia oleracea, Triticum aestivum, Zea mays, Cylindrotheca sp. N1
Manually annotated by BRENDA team
Spreitzer, R.J.
Questions about the complexity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase
Photosynth. Res.
60
29-42
1999
Arabidopsis sp., Chlamydomonas reinhardtii, Glycine max, Helianthus annuus, Hordeum vulgare, Solanum lycopersicum, Marchantia sp., Nicotiana tabacum, Petunia sp., Pisum sativum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Schlitter, J.; Wildner, G.F.
The kinetics of conformation change as determinant of Rubisco's specificity
Photosynth. Res.
65
7-13
2000
Galdieria partita, Rhodospirillum rubrum, Spinacia oleracea, Synechococcus sp.
Manually annotated by BRENDA team
Kim, K.; Portis, A.R., Jr.
Oxygen-dependent H2O2 production by Rubisco
FEBS Lett.
571
124-128
2004
Chlamydomonas reinhardtii, Rhodospirillum rubrum, Spinacia oleracea
Manually annotated by BRENDA team
Kim, K.; Portis, A.R., Jr.
Kinetic analysis of the slow inactivation of Rubisco during catalysis: effects of temperature, O2 and Mg2+
Photosynth. Res.
87
195-204
2006
Spinacia oleracea
Manually annotated by BRENDA team
Liu, C.; Hong, F.S.; Wu, K.; Ma, H.B.; Zhang, X.G.; Hong, C.J.; Wu, C.; Gao, F.Q.; Yang, F.; Zheng, L.; Wang, X.F.; Liu, T.; Xie, Y.N.; Xu, J.H.; Li, Z.R.
Effect of Nd3+ ion on carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase of spinach
Biochem. Biophys. Res. Commun.
342
36-43
2006
Spinacia oleracea
Manually annotated by BRENDA team
McNevin, D.; von Caemmerer, S.; Farquhar, G.
Determining RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model
J. Exp. Bot.
57
3883-3900
2006
Spinacia oleracea
Manually annotated by BRENDA team
Yamori, W.; Suzuki, K.; Noguchi, K.; Nakai, M.; Terashima, I.
Effects of Rubisco kinetics and Rubisco activation state on the temperature dependence of the photosynthetic rate in spinach leaves from contrasting growth temperatures
Plant Cell Environ.
29
1659-1670
2006
Spinacia oleracea
Manually annotated by BRENDA team
Tcherkez, G.G.; Farquhar, G.D.; Andrews, T.J.
Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized
Proc. Natl. Acad. Sci. USA
103
7246-7251
2006
Allochromatium vinosum, Amaranthus hybridus, Trichormus variabilis, Chlamydomonas reinhardtii, Euglena gracilis, Galdieria sulphuraria, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum, Rhodospirillum rubrum, Sorghum bicolor, Spinacia oleracea, Synechococcus sp., Triticum aestivum, Zea mays, Griffithsia monilis, Atriplex glabriuscula, Synechococcus sp. 6301, Synechococcus sp. 7002
Manually annotated by BRENDA team
Xuming, W.; Fengqing, G.; Linglan, M.; Jie, L.; Sitao, Y.; Ping, Y.; Fashui, H.
Effects of nano-anatase on ribulose-1, 5-bisphosphate carboxylase/oxygenase mRNA expression in spinach
Biol. Trace Elem. Res.
126
280-289
2008
Spinacia oleracea
Manually annotated by BRENDA team
Andersson, I.; Backlund, A.
Structure and function of Rubisco
Plant Physiol. Biochem.
46
275-291
2008
Cupriavidus necator, Chlorobaculum tepidum, Galdieria partita, Nicotiana tabacum, Thermococcus kodakarensis, Rhodopseudomonas palustris, Synechococcus sp., Halothiobacillus neapolitanus, Geobacillus kaustophilus, Spinacia oleracea (O20253), Pyrococcus horikoshii (O58677), Rhodospirillum rubrum (P04718), Chlamydomonas reinhardtii (P08475), Oryza sativa (Q0INY7)
Manually annotated by BRENDA team
Raunser, S.; Magnani, R.; Huang, Z.; Houtz, R.L.; Trievel, R.C.; Penczek, P.A.; Walz, T.
Rubisco in complex with Rubisco large subunit methyltransferase
Proc. Natl. Acad. Sci. USA
106
3160-3165
2009
Nicotiana tabacum, Spinacia oleracea
Manually annotated by BRENDA team
Liu, C.; Hong, F.S.; Tao, Y.; Liu, T.; Xie, Y.N.; Xu, J.H.; Li, Z.R.
The mechanism of the molecular interaction between cerium (III) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)
Biol. Trace Elem. Res.
143
1110-1120
2011
Spinacia oleracea
Manually annotated by BRENDA team
Genkov, T.; Meyer, M.; Griffiths, H.; Spreitzer, R.J.
Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in Chlamydomonas
J. Biol. Chem.
285
19833-19841
2010
Arabidopsis thaliana, Chlamydomonas reinhardtii, Helianthus annuus, Spinacia oleracea
Manually annotated by BRENDA team
Carmo-Silva, A.E.; Barta, C.; Salvucci, M.E.
Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves
Methods Mol. Biol.
684
339-347
2011
Arabidopsis thaliana, Chlamydomonas sp., Nicotiana tabacum, Spinacia oleracea, Xanthium sp.
Manually annotated by BRENDA team
Whitney, S.; Houtz, R.; Alonso, H.
Advancing our understanding and capacity to engineer natures CO2-sequestering enzyme, Rubisco
Plant Physiol.
155
27-35
2011
Pyrococcus horikoshii (O58677), Thermococcus kodakarensis (O93627), Spinacia oleracea (P00870), Spinacia oleracea (P00875), Rhodospirillum rubrum (P04718)
Manually annotated by BRENDA team
van Lun, M.; Hub, J.S.; van der Spoel, D.; Andersson, I.
CO2 and O2 distribution in Rubisco suggests the small subunit functions as a CO2 reservoir
J. Am. Chem. Soc.
136
3165-3171
2014
Spinacia oleracea (P00870)
Manually annotated by BRENDA team
Martin, A.; Nieuwland, M.; De Jong, G.
Characterization of heat-set gels from RuBisCO in comparison to those from other proteins
J. Agric. Food Chem.
62
10783-10791
2014
Spinacia oleracea
-
Manually annotated by BRENDA team