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Information on EC 4.1.1.37 - uroporphyrinogen decarboxylase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P32347

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.37 uroporphyrinogen decarboxylase
IUBMB Comments
Acts on a number of porphyrinogens.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P32347
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
uroporphyrinogen decarboxylase, uro-d, porphyrinogen carboxy-lyase, uroporphyrinogen iii decarboxylase, urod protein, uroporphyrinogen-decarboxylase, uro-decarboxylase, hem12p, uroporphyrinogen-iii decarboxylase, tobacco urod, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Decarboxylase, uroporphyrinogen
-
-
-
-
Porphyrinogen carboxy-lyase
-
-
-
-
UPD
-
-
-
-
URO-D
-
-
-
-
Uroporphyrinogen III decarboxylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
uroporphyrinogen-III carboxy-lyase (coproporphyrinogen-III-forming)
Acts on a number of porphyrinogens.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-70-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Uroporphyrinogen III
Coproporphyrinogen III + 4 CO2
show the reaction diagram
-
-
-
?
7-COOH-porphyrinogen I
Coproporphyrinogen I + CO2
show the reaction diagram
-
-
-
-
?
uroporphyrinogen I
coproporphyrinogen I + 4 CO2
show the reaction diagram
-
-
-
-
?
Uroporphyrinogen III
Coproporphyrinogen III + 4 CO2
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Uroporphyrinogen III
Coproporphyrinogen III + 4 CO2
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000007
7-COOH-porphyrinogen I
-
-
0.00001
Uroporphyrinogen I
-
-
0.000006
uroporphyrinogen III
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
uroporphyrinogen I, uroporphyrinogen III or 7-COOH-porphyrinogen I, estimation by coproporphyrinogen formation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
uroporphyrinogen decarboxylase, Hem12p, is involved in the 5th step of heme biosynthesis in the cytosol, before biosynthesis is completed within mitochondria
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
1 * 38000, SDS-PAGE
46000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 38000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutathionylation
Cys52 of the enzyme can be glutathionylated and subsequently deglutathionylated by Grx2p in vitro
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Felix, F.; Brouillet, N.
Purification and properties of uroporphyrinogen decarboxylase from Saccharomyces cerevisiae
Eur. J. Biochem.
188
393-403
1990
Saccharomyces cerevisiae
Manually annotated by BRENDA team
McDonagh, B.; Pedrajas, J.R.; Padilla, C.A.; Barcena, J.A.
Thiol redox sensitivity of two key enzymes of heme biosynthesis and pentose phosphate pathways: uroporphyrinogen decarboxylase and transketolase
Oxid. Med. Cell. Longev.
2013
932472
2013
Saccharomyces cerevisiae (P32347), Saccharomyces cerevisiae
Manually annotated by BRENDA team