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EC Tree
IUBMB Comments This enzyme replenishes oxaloacetate in the tricarboxylic acid cycle when operating in the reverse direction. The reaction proceeds in two steps: formation of carboxyphosphate and the enolate form of pyruvate, followed by carboxylation of the enolate and release of phosphate.
The taxonomic range for the selected organisms is: Flaveria trinervia The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pepck, phosphoenolpyruvate carboxykinase, pepc, phosphoenolpyruvate carboxylase, pepcase, pep carboxylase, c4 pepc, pepc1, phosphoenol pyruvate carboxylase, pep-carboxylase,
more
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Carboxylase, phosphopyruvate (phosphate)
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Phosphoenolpyruvate carboxylase
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Phosphoenolpyruvic carboxylase
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PEPC
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-, -, -, -, -, -, -, -, -, -, -
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phosphate:oxaloacetate carboxy-lyase (adding phosphate; phosphoenolpyruvate-forming)
This enzyme replenishes oxaloacetate in the tricarboxylic acid cycle when operating in the reverse direction. The reaction proceeds in two steps: formation of carboxyphosphate and the enolate form of pyruvate, followed by carboxylation of the enolate and release of phosphate.
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phosphate + oxaloacetate
phosphoenolpyruvate + HCO3-
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?
phosphoenolpyruvate + CO2
phosphate + oxaloacetate
phosphoenolpyruvate + HCO3-
phosphate + oxaloacetate
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?
phosphoenolpyruvate + CO2
phosphate + oxaloacetate
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?
phosphoenolpyruvate + CO2
phosphate + oxaloacetate
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?
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phosphate + oxaloacetate
phosphoenolpyruvate + HCO3-
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?
phosphoenolpyruvate + HCO3-
phosphate + oxaloacetate
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?
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(3-bromophenyl)-4-(3-hydroxybenzylidene)-pyrazolidine-3,5-dione
compound has a selectivity factor of 16.6 over C3 PepC
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
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AG 1433
i.e. 2-(3,4-dihydroxyphenyl)-6,7-dimethylquinoxaline
epigallocatechin gallate
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L-glucose 6-phosphate
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additional information
additional information
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0.103
(+)-catechin
Flaveria trinervia
at pH 8.0 and 25°C
0.00196
(3-bromophenyl)-4-(3-hydroxybenzylidene)-pyrazolidine-3,5-dione
Flaveria trinervia
pH 7.5, 25°C
0.065
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
Flaveria trinervia
at pH 8.0 and 25°C
0.039
AG 1433
Flaveria trinervia
at pH 8.0 and 25°C
8.7
malate
Flaveria trinervia
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wild type enzyme, pH and temperature not specified in the publication
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UniProt
brenda
C4 plant
UniProt
brenda
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brenda
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physiological function
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the enzyme is a key player in C4 photosynthesis
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CAPPA_FLATR
966
0
110406
Swiss-Prot
other Location (Reliability: 3 )
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docking of compound (3-bromophenyl)-4-(3-hydroxybenzylidene)-pyrazolidine-3,5-dione into the binding sites of C3 and C4 PepC
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His-Trap FF or HP column chromatography
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expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
expression in Escherichia coli
expression in Escherichia coli
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Adams, C.A.; Leung, F.; Sun, S.S.M.
Molecular properties of phosphoenolpyruvate carboxylase from C3, C3-C4 intermediate, and C4 Flaveria species
Planta
167
218-225
1986
Flaveria cronquistii, Flaveria floridana, Flaveria trinervia
brenda
Svensson, P.; Blsing, O.E.; Westhoff, P.
Evolution of the enzymatic characteristics of C4 phosphoenolpyruvate carboxylase. A comparison of the orthologous PPCA phosphoenolpyruvate carboxylases of Flaveria trinervia (C4) and Flaveria pringlei (C3)
Eur. J. Biochem.
246
452-460
1997
Flaveria pringlei, Flaveria trinervia
brenda
Paulus, J.K.; Foerster, K.; Groth, G.
Direct and selective small-molecule inhibition of photosynthetic PEP carboxylase: New approach to combat C4 weeds in arable crops
FEBS Lett.
588
2101-2106
2014
Flaveria trinervia (P30694), Flaveria pringlei (Q01647)
brenda
Paulus, J.K.; Niehus, C.; Groth, G.
Evolution of C4 phosphoenolpyruvate carboxylase: enhanced feedback inhibitor tolerance is determined by a single residue
Mol. Plant
6
1996-1999
2013
Flaveria trinervia, Flaveria pringlei (Q01647)
brenda
Schlieper, D.; Frster, K.; Paulus, J.K.; Groth, G.
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase
Mol. Plant
7
437-440
2013
Flaveria trinervia
brenda
Dick, M.; Erlenkamp, G.; Nguyen, G.T.T.; Foerster, K.; Groth, G.; Gohlke, H.
Pyrazolidine-3,5-dione-based inhibitors of phosphoenolpyruvate carboxylase as a new class of potential C
FEBS Lett.
591
3369-3377
2017
Flaveria trinervia (P30694), Flaveria pringlei (Q01647)
brenda