Information on EC 3.9.1.1 - phosphoamidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.9.1.1
-
RECOMMENDED NAME
GeneOntology No.
phosphoamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-phosphocreatine + H2O = creatine + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of P-N bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphamide hydrolase
Also acts on N-phospho-arginine and other phosphoamides. Possibly identical with EC 3.1.3.9 (glucose-6-phosphatase) or EC 3.1.3.16 (protein-serine/threonine phosphatase).
CAS REGISTRY NUMBER
COMMENTARY hide
9001-79-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-N-phosphoryl-DL-tryptophan methyl ester + H2O
DL-tryptophan methyl ester + phosphate
show the reaction diagram
-
-
-
-
?
creatine phosphate + H2O
creatine + o-phosphate
show the reaction diagram
-
-
-
-
?
creatine phosphate + H2O
creatine + phosphate
show the reaction diagram
-
-
-
-
?
N-(p-chlorophenyl)phosphoramidate + H2O
4-chlorophenylamine + phosphate
show the reaction diagram
-
-
-
-
?
N-(p-tolyl)phosphoramidate + H2O
4-aminophenol + phosphate
show the reaction diagram
-
-
-
-
?
N-phosphoglycine + H2O
glycine + phosphate
show the reaction diagram
-
-
-
-
?
o-carboxyphenylphosphate + H2O
salicylic acid + phosphate + CO2
show the reaction diagram
-
-
-
-
?
O-phospo-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
phosphoramidate + H2O
amidate + phosphate
show the reaction diagram
-
cleavage of the N-P bond
-
-
?
phosphoramidate + H2O
NH4+ + phosphate
show the reaction diagram
phosvitin + H2O
?
show the reaction diagram
-
-
-
-
?
uridine 5'-N-alkylphosphoramidate + H2O
?
show the reaction diagram
-
overview, several different substrates
-
-
?
uridine 5'-N-arylphosphoramidate + H2O
?
show the reaction diagram
-
overview, several different substrates
-
-
?
uridine-5'-phosphoro-p-anisidine + H2O
uridine + p-anisidine + phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
creatine phosphate + H2O
creatine + o-phosphate
show the reaction diagram
-
-
-
-
?
phosphoramidate + H2O
amidate + phosphate
show the reaction diagram
-
cleavage of the N-P bond
-
-
?
uridine 5'-N-alkylphosphoramidate + H2O
?
show the reaction diagram
-
overview, several different substrates
-
-
?
uridine 5'-N-arylphosphoramidate + H2O
?
show the reaction diagram
-
overview, several different substrates
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe2+
-
40% inhibition
Fe3+
-
30% inhibition
fluoride
-
-
Iodosobenzene
-
-
molybdate
-
-
p-chloromercuribenzoate
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-
phosphate
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concentration higher than 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sulfhydryl compounds
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i.e. beta-mercaptoethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13
Phosphoramidate
-
-
0.8
uridine-5'-phosphoro-benzylamine
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-
1
uridine-5'-phosphoro-beta-phenyl-isopropylamine
-
-
1.85
uridine-5'-phosphoro-cyclohexylamine
-
-
0.28
uridine-5'-phosphoro-dimethylamine
-
-
0.75
uridine-5'-phosphoro-isopropylamine
-
-
0.1
uridine-5'-phosphoro-n-butylamine
-
-
1.8
uridine-5'-phosphoro-p-aniline
-
-
1
uridine-5'-phosphoro-p-anisidine
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-
0.09
uridine-5'-phosphoro-trifluoroethylamine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.16
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substrate phosphoramidate, enzyme source kidney microsomes
0.181
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substrate phosphoramidate, enzyme source liver microsomes
2.2
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substrate phosvitin, measurement of phosphate
4.8
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substrate phosphoramidate, measurement of NH4+
additional information
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specific activity 38 enzyme units per mg protein with substrate o-carboxyphenylphosphate, 1 enzyme unit defined as amount of enzyme causing absorbance change per 5 min, measurement of salicylic acid
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
about
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
pretreatment with pH 5.0 reduces activity 25fold
247110
9.7
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pretreatment with pH 9.7 increases activity 1.4fold, KM decreases 5.7fold
247110
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
heating for 5 min does not destroy the enzyme activity or precipitate the enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is active for more than 5 h, shows activity even at 0°C
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, lyophilized, 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
50fold
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