Information on EC 3.8.1.6 - 4-chlorobenzoate dehalogenase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.8.1.6
-
RECOMMENDED NAME
GeneOntology No.
4-chlorobenzoate dehalogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4-chlorobenzoate + H2O = 4-hydroxybenzoate + chloride
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of C-halide
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-chlorobenzoate chlorohydrolase
Catalyses the first step in the degradation of chlorobenzoate in Pseudomonas. In many microorganisms, this activity comprises three separate enzymes, EC 6.2.1.33 (4-chlorobenzoate---CoA ligase), EC 3.8.1.7 (4-chlorobenzoyl-CoA dehalogenase) and EC 3.1.2.23 (4-hydroxybenzoyl-CoA thioesterase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
FcbB
O68600
-
FcbB
O68600
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ST-1
-
-
Manually annotated by BRENDA team
Acinetobacter sp. ST-1
strain ST-1
-
-
Manually annotated by BRENDA team
strain SU DSM 20407
-
-
Manually annotated by BRENDA team
strain SU DSM 20407
-
-
Manually annotated by BRENDA team
strain LB400 BphK, additional activity of glutathione-s-transferase
SwissProt
Manually annotated by BRENDA team
Burkholderia sp. LB400 BphK
strain LB400 BphK, additional activity of glutathione-s-transferase
SwissProt
Manually annotated by BRENDA team
strain DJ-12, enzyme belongs to enoyl-CoA hydratase superfamily
SwissProt
Manually annotated by BRENDA team
strain DJ-12, enzyme belongs to enoyl-CoA hydratase superfamily
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-chloro-2,4-dinitrobenzene + H2O
2,4-dinitrophenol + chloride
show the reaction diagram
Burkholderia sp., Burkholderia sp. LB400 BphK
Q59721
-
-
-
?
4-bromobenzoate + H2O
4-hydroxybenzoate + Br-
show the reaction diagram
-
dehalogenating activity in decreasing order: 4-chlorobenzoate, 4-bromobenzoate, 4-iodobenzoate
-
?
4-bromobenzoate + H2O
4-hydroxybenzoate + Br-
show the reaction diagram
-
relative activity compared to 4-chlorobenzoate, 55%
-
?
4-bromobenzoate + H2O
4-hydroxybenzoate + Br-
show the reaction diagram
Acinetobacter sp. ST-1
-
dehalogenating activity in decreasing order: 4-chlorobenzoate, 4-bromobenzoate, 4-iodobenzoate
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
Q59721
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
Burkholderia sp. LB400 BphK
Q59721
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
Acinetobacter sp. ST-1
-
-
-
?
4-iodobenzoate + H2O
4-hydroxybenzoate + I-
show the reaction diagram
-
-
-
?
4-iodobenzoate + H2O
4-hydroxybenzoate + I-
show the reaction diagram
-
relative activity compared to 4-chlorobenzoate, 120%
-
?
4-iodobenzoate + H2O
4-hydroxybenzoate + I-
show the reaction diagram
Acinetobacter sp. ST-1
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
-
-
-
?
4-chlorobenzoate + H2O
4-hydroxybenzoate + Cl-
show the reaction diagram
Acinetobacter sp. ST-1
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
CuSO4
-
1 mM, 73.4% inhibition
ZnSO4
-
1 mM, 30.4% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
H2O2
-
1mM, 20% activation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.056
-
4-Bromobenzoate
-
-
0.12
-
4-Chlorobenzoate
-
-
0.086
-
4-Iodobenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.5
-
-
cell-free extracts
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
room temperature, 4 h, 50%, 4°C, 20 h, 50%, -18°C, 2 weeks, 50%
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gel filtration, yield 5-10%
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
complete gene cluster responsible for hydrolytic dechlorination of 4-chlorobenzoate
O68600
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A154R
Q59721
84% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 57% of wild-type activity with 4-chlorobenzoate
A154S
Q59721
84% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 47% of wild-type activity with 4-chlorobenzoate
D155Y
Q59721
no decrease in glutathione S-transferase actvitiy, affected in thermostability, 96% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 95% of wild-type activity with 4-chlorobenzoate
L158P
Q59721
56% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 50% of wild-type activity with 4-chlorobenzoate
S152G
Q59721
4% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 3% of wild-type activity with 4-chlorobenzoate
S152I
Q59721
53% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 156% of wild-type activity with 4-chlorobenzoate
V153I
Q59721
56% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 52% of wild-type activity with 4-chlorobenzoate
V153R
Q59721
4% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 39% of wild-type activity with 4-chlorobenzoate
Y157W
Q59721
49% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 59% of wild-type activity with 4-chlorobenzoate
L158P
Burkholderia sp. LB400 BphK
-
56% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 50% of wild-type activity with 4-chlorobenzoate
-
S152G
Burkholderia sp. LB400 BphK
-
4% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 3% of wild-type activity with 4-chlorobenzoate
-
S152I
Burkholderia sp. LB400 BphK
-
53% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 156% of wild-type activity with 4-chlorobenzoate
-
V153I
Burkholderia sp. LB400 BphK
-
56% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 52% of wild-type activity with 4-chlorobenzoate
-
V153R
Burkholderia sp. LB400 BphK
-
4% of wild-type activity with 1-chloro-2,4-dinitrobenzene, 39% of wild-type activity with 4-chlorobenzoate
-