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Information on EC 3.6.5.2 - small monomeric GTPase and Organism(s) Pisum sativum and UniProt Accession Q41009

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IUBMB Comments
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
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Pisum sativum
UNIPROT: Q41009
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Word Map
The taxonomic range for the selected organisms is: Pisum sativum
The enzyme appears in selected viruses and cellular organisms
Synonyms
k-ras, cdc42, h-ras, small gtpase, ef-tu, rho gtpase, rab11, rab3a, rab27a, rap1a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GTPase Toc33/34
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GTP-phosphohydrolase
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-
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GTPase
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-
-
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guanine triphosphatase
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-
-
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guanosine 5'-triphosphatase
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-
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guanosine triphosphatase
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-
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Pra2
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member of the Ypt3/Rab11 family
Pra3
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member of the Ypt3/Rab11 family
ribosomal GTPase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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-
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SYSTEMATIC NAME
IUBMB Comments
GTP phosphohydrolase (cell-regulating)
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
CAS REGISTRY NUMBER
COMMENTARY hide
9059-32-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope
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-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
-
acts on the intracellular membrane through the trafficking pathway
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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etiolated
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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recombinant Pra2 is predominantly localized on Golgi stacks and endosomes
Manually annotated by BRENDA team
additional information
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subcellular localization
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Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TOC34_PEA
310
0
34157
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
the small GTPase Toc33/34 can homodimerize. Dimerization is independent of nucleotide loading state. GTPase activation by dimerization is sparse. The dimer is not self-activating, possibly lacking an additional regulatory layer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state of Toc33/34 GTPase
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the cytosolic G domain of psToc34 (lacking the C-terminal membrane anchor) is purified mainly in the GDP-bound form after recombinant protein production in Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Pra2 cDNA, expression in tobacco Bright Yellow 2 cells
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Pra3 cDNA, expression in tobacco Bright Yellow 2 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Inaba, T.; Nagano, Y.; Nagasaki, T.; Sasaki, Y.
Distinct localization of two closely related Ypt3/Rab11 proteins on the trafficking pathway in higher plants
J. Biol. Chem.
277
9183-9188
2002
Pisum sativum
Manually annotated by BRENDA team
Koenig, P.; Oreb, M.; Hoefle, A.; Kaltofen, S.; Rippe, K.; Sinning, I.; Schleiff, E.; Tews, I.
The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures
Structure
16
585-596
2008
Arabidopsis thaliana (O23680), Arabidopsis thaliana, Pisum sativum (Q41009), Pisum sativum
Manually annotated by BRENDA team