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Information on EC 3.6.5.2 - small monomeric GTPase and Organism(s) Arabidopsis thaliana and UniProt Accession O23680

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IUBMB Comments
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O23680
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
k-ras, cdc42, h-ras, small gtpase, ef-tu, rho gtpase, rab11, rab3a, rab27a, rap1a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AtRopGEF12
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-
GTP-phosphohydrolase
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-
-
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GTPase
guanine triphosphatase
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-
-
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guanosine 5'-triphosphatase
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-
-
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guanosine triphosphatase
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-
-
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RabE1d
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isoform
ribosomal GTPase
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-
-
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small G protein
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small GTPase LIP1
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
GTP phosphohydrolase (cell-regulating)
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
CAS REGISTRY NUMBER
COMMENTARY hide
9059-32-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
nucleoside diphosphate kinase-2
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EC 2.7.4.6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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epidermal cell
Manually annotated by BRENDA team
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signal relay in which pollen receptor kinase AtPRK2a at the plasma membrane recruits AtRopGEF12 through its C terminus to maintain polar Rop activity in the pollen tube
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the subcellular localization of LIP1 is not significantly affected by light
Manually annotated by BRENDA team
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the subcellular localization of LIP1 is not significantly affected by light
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TOC33_ARATH
297
0
32925
Swiss-Prot
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state and GMP-bound state of Toc33 GTPase
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q74L
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the mutant is preferentially GTP bound and therefore in an active state
S29N
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the mutant is preferentially GDP bound and therefore in an inactive state
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
mutant enzymes Q47L and S29N are expressed in Arabidopsis thaliana
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhao, Q.; Leung, S.; Corbett, A.H.; Meier, I.
Identification and characterization of the Arabidopsis orthologs of nuclear transport factor 2, the nuclear import factor of ran
Plant Physiol.
140
869-878
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Kevei, E.; Gyula, P.; Feher, B.; Toth, R.; Viczian, A.; Kircher, S.; Rea, D.; Dorjgotov, D.; Schaefer, E.; Millar, A.J.; Kozma-Bognar, L.; Nagy, F.
Arabidopsis thaliana circadian clock is regulated by the small GTPase LIP1
Curr. Biol.
17
1456-1464
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Zhang, Y.; McCormick, S.
A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
104
18830-18835
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Koenig, P.; Oreb, M.; Hoefle, A.; Kaltofen, S.; Rippe, K.; Sinning, I.; Schleiff, E.; Tews, I.
The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures
Structure
16
585-596
2008
Arabidopsis thaliana (O23680), Arabidopsis thaliana, Pisum sativum (Q41009), Pisum sativum
Manually annotated by BRENDA team
Shen, Y.; Han, Y.J.; Kim, J.I.; Song, P.S.
Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein
BMB Rep.
41
645-650
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Speth, E.B.; Imboden, L.; Hauck, P.; He, S.Y.
Subcellular localization and functional analysis of the Arabidopsis GTPase RabE
Plant Physiol.
149
1824-1837
2009
Arabidopsis thaliana
Manually annotated by BRENDA team