Information on EC 3.6.4.11 - nucleoplasmin ATPase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.6.4.11
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RECOMMENDED NAME
GeneOntology No.
nucleoplasmin ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O = ADP + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (nucleosome-assembling)
An acidic nuclear protein that is active in the ATP-dependent assembly of nucleosome cores, in decondensation of sperm chromatin and in other histone-involving processes.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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CHD4 chromatin remodelling factor
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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activates, required
additional information
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in vitro, Rea1-dependent release of Rsa4 and Nug2 requires only ATP and K+ without the addition of GTP, whereas the mutational analyses suggests that GTPase activity is necessary for Nug2 release
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-Amanitin
impairs the binding of the enzyme to DNA damage sites
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DNA
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the ATPase activity of ISWI is activated by free and nucleosomal DNA. DNA ligands strongly influence the ATP hydrolysis mechanism, DNA binding to the ATPase domain activates ATP hydrolysis affecting the conformation of the ATPase domain
dsDNA
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the isolated ATPase domain and tandem chromodomain/tandem plant zinc finger homeodomain/ATPase domain show weak ATPase activity in the absence of DNA, addition of DNA significantly stimulates the activity of the recombinant tPHDtCHD/ATPase construct (3fold kcat increase at saturating DNA concentration) whereas it has no effect on the activity of the ATPase domain alone. This stimulation is specific for dsDNA since no effect is observed in the presence of RNA(polyA)
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histone H4
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an N-terminal peptide of histone H4 activates ISWI ATP turnover, stimulation of ATP turnover by nucleosomes requires the histone H4 N-terminal tail
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.0615
ATP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.57
AMPPNP
Drosophila melanogaster
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pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.81
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full length nucleoplasmin, calculated
5.03
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core domain, calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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subset positive: 50%, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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Purkinje cell and dentate nucleus, no activity in internal granular layer, stellate, basket, Golgi, arachnoidal cells and glial cells; Purkinje cell, dentate nucleus, internal granular layer, stellate, basket, arachnoidal cells and glial cells, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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epithelium, muscularis propria and ganglion cells, no activity in endothelium and submucosa, SWI/SNF ATPase subunit BRM; epithelium, muscularis propria and ganglion cells: subset positive (50%), no activity in muscularis mucosa, submucosa and endothelium, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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isoform nucleoplasmin 3 is expressed at high level, expression is reduced during retinoic acid-induced differentiation
Manually annotated by BRENDA team
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epithelium, stroma, lunica media, endothelium, myocardium, epicardium. No activity in endocardium and pericardial fat, SWI/SNF ATPase subunit BRM; epithelium: subset positive (50%), no activity in stroma epithelium myocardium, endocardium, epicardium, endothelium and pericardial fat, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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bronchial epithelium, arterial endothelium, and alveolar type I and type II cells, SWI/SNF ATPase subunit BRM; bronchial epithelium: subset poitive, no activity in arterial endothelium, and alveolar type I and type II cells, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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MyoD and Brg1 co-localize to the myogenin promoter in primary adult muscle satellite cells. In mature myofibers, myogenin and Brg1 are preferentially co-localized to the myogenin promoter. In cultured cells, myogenin, in the absence of MyoD, is capable of maintaining its own expression by recruiting the Brg1 ATPase to modify promoter chromatin structure and facilitate myogenin expression. In newborn skeletal muscle tissue, Brg1 is required for the continued production of the myogenin protein
Manually annotated by BRENDA team
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surface epithelium and stroma, SWI/SNF ATPase subunit BRM; surface epithelium: subset positive (20%), no activity in stroma, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
small cell carcinoma of the ovary, hypercalcaemic type (SCCOHT), expression analysis of SMARCA4 by immunohistochemistry in more than 3000 primary gynaecological tumors, including the SCCOHT cell lines BIN67 and SCCOHT1. All SMARCA4-negative SCCOHTs also lack SMARCA2 protein; small cell carcinoma of the ovary, hypercalcaemic type (SCCOHT), expression analysis of SMARCA4 by immunohistochemistry in more than 3000 primary gynaecological tumours, including the SCCOHT cell lines BIN67 and SCCOHT1. All SMARCA4-negative SCCOHTs also lack SMARCA2 protein
Manually annotated by BRENDA team
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ductal epithelium and acinar epithelium: subset poitive (50%), islet cells: subset positive (20%), SWI/SNF ATPase subunit BRG1; ductal epithelium and acinar epithelium, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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parenchyma: subset positive (20%), SWI/SNF ATPase subunit BRG1; parenchyma, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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retinal stem cell
Manually annotated by BRENDA team
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ductal epithelium, acinar epithelium, SWI/SNF ATPase subunit BRM; ductal epithelium: subset positive (20%), no activity in acinar epithelium and adipocytes, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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suprabasal and basal epidermis, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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epithelium and submucosa, no activity in endothelium, SWI/SNF ATPase subunit BRM; epithelium: subset positive (50%), SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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primary and secondary, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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SWI/SNF ATPase subunit BRG1; SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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lymphoid germinal centers and sinusoidal cell in red pulp, no activity in central artery and other enothelia, SWI/SNF ATPase subunit BRG1; lymphoid germinal centers, sinusoidal cell in red pulp and central artery and other enothelia, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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lymphoid follicles and paracortical lymphoid tissue, SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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urothelium, lamina propria and muscularis propria, SWI/SNF ATPase subunit BRM; urothelium: subset positive (90%), lamina propria: subset positive (50%), SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
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SWI/SNF ATPase subunit BRM
Manually annotated by BRENDA team
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endometrial epithelium, endometrial stroma, myometrium and vessel epithelium, SWI/SNF ATPase subunit BRM; endometrial epithelium: subset positive, no activity in endometrial stroma, myometrium and myometrium vessel epithelium, SWI/SNF ATPase subunit BRG1
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20120
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calculated from cDNA sequence
23000
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degradation product of NO29, 2D gel electrophoresis
24000
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degradation product of NO29, 2D gel electrophoresis
31000 - 33000
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gel filtration
53000
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SDS-PAGE
55000
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59000
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100000
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sucrose density gradient centrifugation, gel filtration
150000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homopentamer
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trimer
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3 * 53000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal strucuture shows a largely helical protein with a small antiparallel beta-sheet in the ZA loop region. The BRG1 bromodomain binds weakly to acetylated peptides derived from histones H3 and H4, modeling of the peptides into the BRG1 structure
activated core domain
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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highest value of thermal stability
696244
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
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resistant to treatment for 10 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme by affinity and ion exchange chromatography, and gel filtration
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immunopurification
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NAP-1 from FM3A cells
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native and recombinant dNLP
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nucleoplasmin and NAP-1
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SWI/SNF complex
wild-type SWI/SNF complex, delta-bromodomain SWI/SNF complex
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA encoding dNLP, subcloned into pET15b to give the bacterial expression plasmid pETdNLP, expression in Escherichia coli Bl21(DE3)
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expression of amino acid residues 1568–1919 of Arabidopsis BRM fused to glutathione-S-transferase, and of parts of this stretch, in Escherichia coli
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expression of FLAG epitope-tagged BRG1 DN construct in HEK 293 cells
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full-length cDNA sequence
full-length nucleoplasmin and wtCORE
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gene brm, quantitative real-time RT-PCR enzyme expression analysis
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NAP-1, recombinant versions of the protein function in nucleosome assembly
NO29 cDNA cloning
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recombinant expression of enzyme CHD4 domain constructs
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recombinant expression of GFP-tagged SMARCA5 in U2O2 cells, co-expression of GFP-tagged SMARCA5 with mCherry-fused Potorous tridactylus CPD-photolyase, SMARCA5 moves away from the area with the highest damage concentration and accumulates at the periphery
SMARCA2 real-time RT-PCR enzyme expression analysis; SMARCA4 real-time RT-PCR enzyme expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
HDAC inhibitor trichostatin A induces the SMARCA2 enzyme expression, while DNA methyltransferase inhibitor 5-azacytidine and the EZH2 histone methyltransferase inhibitor GSK343 are not inducing
HDAC-mediated epigenetic silencing of SMARCA2 in small cell carcinoma of the ovary, hypercalcaemic type, SCCOHT, cells, or an indirect inhibitory effect on SMARCA2 mRNA degradation
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E257Q
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site-directed mutagenesis
DELTA96-132
deletion mutant lacking residues 96-132 and a deletion mutant lacking the N-terminal domain as well as residues 96-132 can still bind TATA box binding protein (TBP), indicating that EcTBP is mainly bound by acidic loops of Mot1's HEAT repeats 4-6
K211R
site-directed mutagenesis, inactive SMARCA5 mutant
E1083G
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mutation in a highly conserved region of the catalytic ATPase domain. The mutant protein is stable, assembles into SWI/SNF-related complexes, and exhibits normal ATPase activity, but its nucleosome remodelling properties are diminished. Mutant embryos develop normally, until midgestation, but then exhibit a distinct block in the development of the erythroid lineage, leading to anemia and death
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine