Information on EC 3.6.3.B5 - anion transporter (cotransporter-dependent)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.3.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
anion transporter (cotransporter-dependent)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
miniature pig
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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patients with infantile sialic acid storage disorder, ISSD, show the effect of the Salla disease and ISSD sialin mutations on anion transport displaying reduced sialic acid- and NO3-induced currents compared with healthy controls. Expression of disease-associated sialin mutants in fibroblasts and salivary gland cells suppressed the H+-dependent NO3? conductance
metabolism
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sialin protein level increases when the autophagy process is inhibited
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetylaspartylglutamate/out + ?
N-acetylaspartylglutamate/in + ?
show the reaction diagram
-
-
-
?
N-acetylaspartylglutamylglutamate /out + ?
N-acetylaspartylglutamylglutamate/in + ?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid
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9-deoxy-9-iodo-2,4,7,8-tetra-O-propanoyl-N-acetylneuraminic acid
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N-acetylneuraminic acid
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021 - 0.5
9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid
0.068
9-deoxy-9-iodo-2,4,7,8-tetra-O-propanoyl-N-acetylneuraminic acid
Homo sapiens
Q9NRA2
pH 5.0, 22C
0.83 - 1.38
N-acetylneuraminic acid
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
transport assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
sialin is localized in the basolateral regions of human parotid gland biopsies
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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x * 80000, glycosyated protein, x * 40000, deglycosylated protein
80000
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x * 80000, glycosyated protein, x * 40000, deglycosylated protein
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 80000, glycosyated protein, x * 40000, deglycosylated protein
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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PNGase F treatment leads to a decrease in molecular mass from 80000 Da to 40000 Da
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cytosol-open and lumen-open three-dimensional models of sialin based sequence similarity with the glycerol-3-phosphate and fucose permeases from Escherichia coli, respectively, and molecular docking of synthetic sialic acid analogues. Narrowing the sialic acid-binding site in the cytosol-open state by two phenylalanine to tyrosine mutations abrogates recognition of the most active analogue without impairing neuraminic acid transport
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HEK-293 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F410Y
mutation causes partial, selective loss of analogue 9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid recognition
F50A/F410A
mutation has no significant effect on both N-acetylneuraminic acid and analogue 9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid
F50Y
mutation causes partial, selective loss of analogue 9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid recognition
F50Y/F410Y
mutant is able to translocate N-acetylneuraminic acid. Mutation strongly impairs recognition of analogue 9-deoxy-9-iodo-2,4,7,8-tetra-O-acetyl-N-acetylneuraminic acid
L22A/L23A
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naturally occuring mutations in sialin leading to reduced anion transport, overview