Information on EC 3.6.3.9 - Na+/K+-exchanging ATPase

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The expected taxonomic range for this enzyme is: Bilateria

EC NUMBER
COMMENTARY hide
3.6.3.9
-
RECOMMENDED NAME
GeneOntology No.
Na+/K+-exchanging ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphate bond
-
-
-
-
hydrolysis of phosphonic ester bond
-
-
hydrolysis of phosphoric ester
transmembrane transport
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (Na+/K+-exchanging)
A P-type ATPase that undergoes covalent phosphorylation during the transport cycle. This is a plasma membrane enzyme, ubiquitous in animal cells, that catalyses the efflux of three Na+ and influx of two K+ per ATP hydrolysed. It is involved in generating the plasma membrane electrical potential.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
alpha-1 subunit
UniProt
Manually annotated by BRENDA team
dogfish
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Frog
-
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Manually annotated by BRENDA team
juvenile shrimp fed diet rich/low in highly unsaturated fatty acids
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Manually annotated by BRENDA team
freshwater shrimp
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
alpha-subunit
UniProt
Manually annotated by BRENDA team
subunit alpha-1b
UniProt
Manually annotated by BRENDA team
Pareledone sp.
alpha-subunit
UniProt
Manually annotated by BRENDA team
alpha-subunit
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
acclimation from fresh water to seawater is accompanied by 8fold increase in enzyme activity and 3fold increase in enzyme protein number. Enzyme activity in seawater fish is negatively correlated with basolateral membrane cholesterol content and positively correlated with phosphatidylethanolamine and linoleic acid content. Activity in freshwater fish is not correlated to any membrane lipid parameter
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Manually annotated by BRENDA team
shark
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Manually annotated by BRENDA team
Senegalese sole
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Torpedo californica
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphate + 3 Na+/out
ATP + 3 Na+/in
show the reaction diagram
-
in the absence of K+ and in the presence of ATP and ADP without net ATP hydrolysis
transphosphorylation of ATP/ADP
-
?
ADP + phosphate + Na+/out
ATP + H2O + Na+/in
show the reaction diagram
ATP + 3 Na+/in
ADP + phosphate + 3 Na+/out
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O + 3 Na+/in + 2 K+/out
ADP + phosphate + 3 Na+/out + 2 K+/in
show the reaction diagram
ATP + H2O + K+/in
ADP + phosphate + K+/out
show the reaction diagram
ATP + H2O + Na+/in
ADP + phosphate + Na+/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + Na+/in + K+/out
ADP + phosphate + Na+/out + K+/in
show the reaction diagram
ATP + H2O + Na+/in + Rb+/out
ADP + phosphate + Na+/out + Rb+/in
show the reaction diagram
ATP + H2O + Na+in + K+out
ADP + phosphate + Na+out + K+in
show the reaction diagram
ATP + Na+/in
ADP + phosphate + Na+/out
show the reaction diagram
beta-(2-furyl)acryloyl phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + phosphate + Na+/out
ATP + H2O + Na+/in
show the reaction diagram
ATP + H2O + 3 Na+/in + 2 K+/out
ADP + phosphate + 3 Na+/out + 2 K+/in
show the reaction diagram
ATP + H2O + K+/in
ADP + phosphate + K+/out
show the reaction diagram
ATP + H2O + Na+/in + K+/out
ADP + phosphate + Na+/out + K+/in
show the reaction diagram
ATP + H2O + Na+in + K+out
ADP + phosphate + Na+out + K+in
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
activates
Ca2+
-
activates
Cr3+
-
causes oxidative stress in fish and leads to reduced enzyme activity in the brain, mechanism, overview. The level of ATP production in brain mitochondria may be an important criteria in the CrIII induced inhibition of Na+K+ATPase activity of fish brain, reduction of Na+K+ATPase activity is partially mediated by lipid peroxidation and deficient mitochondrial electron transport chain activity. Also direct interaction of CrIII ion with the -SH group of Na+K+ATPase enzyme might play a role
Cs+
-
the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+
Li+
-
the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+
Rb+
-
the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-2,3a,11,11a-tetrahydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
-
-
(1R,2R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9-(hydroxymethyl)-15a-methyl-1-(5-oxo-2,5-dihydrofuran-3-yl)-2-(2-oxopropanoyl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
-
-
(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-13a-formyl-3a,11a-dihydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-11-yl beta-D-glucopyranoside
-
-
(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
-
-
(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9-(hydroxymethyl)-15a-methyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
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1,8,9-trihydroxy-3-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
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i.e. wedelolactone, inhibitor of kidney Na+K+-ATPase and ligand for the central benzodiazepine receptor
1-chloro-2,4-dinitrobenzene
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inhibition of the transport activity of the Na-K pump
12,20-dihydroxydammar-24-en-3-yl 2-O-D-glucopyranosyl-D-glucopyranoside
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12,20-dihydroxydammar-24-en-3-yl D-glucopyranoside
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19-hydroxy-2''-oxovoruscharin
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i.e. UNBS1450, a trans-trans-cis cardiotonic steroid
1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one
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ODQ, inhibition of SNP-induced pump stimulation
2,3-dihydroxy-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-6-one
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inhibitor of kidney Na+K+-ATPase and ligand for the central benzodiazepine receptor
2,8,9-trihydroxy-3-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
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inhibitor of kidney Na+K+-ATPase and ligand for the central benzodiazepine receptor
2,9-dihydroxy-3,8-dimethoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
-
inhibitor of kidney Na+K+-ATPase and ligand for the central benzodiazepine receptor
2-aminoethyl-methanethiosulfonate
-
inhibition of mutants F323C, P333C, E334C, G335C, I322C, G326C and A330C
3,8,9-trihydroxy-2-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
-
inhibitor of kidney Na+K+-ATPase and ligand for the central benzodiazepine receptor
3-[(2-O-D-glucopyranosyl-D-glucopyranosyl)oxy]-12-hydroxydammar-24-en-20-yl D-glucopyranoside
-
-
4,7-diacetoxy-14-hydroxydolast-1(15),8-diene
-
-
4-acetoxy-9,14-dihydroxydolast-1(15),7-diene
-
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4-[(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15R,15aS)-3a,11,11a,15-tetrahydroxy-13a-(hydroxymethyl)-9,15a-dimethylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
-
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4-[(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9,13a-bis(hydroxymethyl)-15a-methylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
-
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4-[(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15R,15aS)-3a,11,11a,15-tetrahydroxy-9,13a-bis(hydroxymethyl)-15a-methylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
-
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5-hydroxydecanoate
-
presence of 5 mM ATP, inhibitory above 1 mM. Presence of 0.05 mM ATP, inhibitory above 0.25 mM. No effect on ouabain-sensitiviy of the enzyme
8-methoxycoumestrol
-
inhibits the isozyme alpha1beta1 Na,K-ATPase, mechanism, overview
A-769662
-
inhibits the Na+-K+-ATPase transport activity and cell surface abundance in L6 cells, which is independent of AMP kinase activation, overview
alpha2-adrenergic agonists
-
negative modulator
-
astragaloside III
-
from dried roots of Astragalus membraneceus or var. mongholicus
ATP
-
competitive inhibitor of K+-phosphatase activity
AuCl4-
-
-
BAY-K8644
benzyltriethylammonium chloride
-
inhibition of pump current is dependent on membrane potential
beta-acetyldigoxin
-
-
bradykinin
-
negative modulator
bufalin
chlorpromazine
-
-
cholic acid
-
from dried bile of Ursus arctos or Selenarctos thibetanus
cinobufotalin
-
27.5% inhibition at 0.01 mM
cyclosporin A
-
diminishs induction of alpha1 protein in activated lymphocytes
dammar-24-ene-3,12,20-triol
-
-
dammar-24-ene-3,6,12,20-tetrol
-
-
dansylcysteinyl-mercuric chloride
-
-
digitoxigenin
digitoxin
-
-
digoxin
dihydroouabain
-
-
diphenyl diselenide
-
activity is restored by DTT
dopamine
-
negative modulator
endobain E
-
-
-
endothelin
-
negative modulator
-
epicatechin
-
-
epicatechin-3-gallate
-
solubilization of the Na+,K+-ATPase with a nonionic detergent reduces sensitivity to epigallocatechin-3-gallate
epigallocatechin
-
-
epigallocatechin-3-gallate
-
noncompetitive with respect to ATP, reduces the affinity for vanadate, shifts the equilibrium of E1P and E2P toward E1P, and reduces the rate of the E1P to E2P transition
Ethacrynic acid
ethanol
-
inhibits the enzyme in Golgi membranes of cerebellar neurons leading to potentiation of GABAergic transmission at Golgi cell-to-granule cell synapses throughan increase in Golgi cell excitability
ethylester of glutathione
-
inhibition of the transport activity of the Na-K pump
ethylmercurithiosalicylate
-
-
fenoldopam
-
induces inhibition of Na+ ?K+-ATPase activity in HK-2 cells. Ouabain protects against the cyclic adenosine monophosphate accumulation and Na+ ?K+-ATPase inhibition induced by the D1 receptor agonist fenoldopam in HK-2 cells. Chronic ouabain treatment decreases the protein and mRNA expression levels of the D1 receptor and increases the basal phosphorylation of the D1 receptor in HK-2 cells
foliandrin
-
-
genistein
-
tyrosine kinase inhibitor, block phosphorylation of alpha-subunit of the Na+,K+-ATPase
ginsenoside Rh2
-
from dried roots of Panax ginseng or Panax notoginseng
glibenclamide
-
-
glyburide
-
presence of 5 mM ATP, slightly stimulating up to 1 mM, inhibitory above. Presence of 0.05 mM ATP, stimulating at least up to 3 mM
glycyrrhizin
-
from roots of Glycyrrhiza uralensis , Glycyrrhiza inflata, or Glycyrrhiza glabra
herbimycin A
-
tyrosine kinase inhibitor, block phosphorylation of alpha-subunit of the Na+,K+-ATPase
isovaleric acid
-
isovaleric acid injection significantly inhibits Na+,K+-ATPase activity by 25% in cerebral cortex of rats 2 or 24 h after administration, while pre-treatment of rats with creatine completely prevents the inhibitory effects of isovaleric acid on Na+,K+-ATPase
jujuboside B
-
from mature seeds of Ziziphus jujube var. spinosa
L-homocysteine
-
inhibtory effect is reversed by alanine
L-phenylalanine
-
-
lauric acid
-
presence of 5 mM ATP, inhibitory above 0.25 mM. Presence of 0.05 mM ATP, stimulating up to 0.3 mM, inhibitory above
lavendustin A
-
tyrosine kinase inhibitor, block phosphorylation of alpha-subunit of the Na+,K+-ATPase
Lyn kinase
-
Lyn kinase directly binds to the Na+,K+-ATPase alpha3 subunit for regulation of activity
-
m-trifluoromethyl-diphenyl diselenide
-
i.e. (m-CF3C6H4Se)2, activity is restored by DTT
marinobufagenin
-
-
methyldigoxin
-
-
Mg2+
-
IC50: 0.0008 mM; IC50: 0.0012 mM; IC50: 0.012 mM
MTSET
-
[2-(trimethylammonium)ethyl]methanethiosulphonate bromide, inhibits mutants G803C, V805C
Na+
-
25% inhibition at 80 mmol/l
Na/K ATPase-alpha1-specific siRNA A1
Na/K ATPase-alpha1-specific siRNA A2
Na/K ATPase-alpha1-specific siRNA A3
Na/K ATPase-alpha1-specific siRNA A4
Na2CO3
-
inactivation
odoroside A
-
i.e. 3beta-O-(beta-D-diginosyl)-14-hydroxy-5beta,14beta-card-20(22)-enolide, isolated from the stems and twigs of Nerum oleander, inhibits the enzyme's ATPase activity
oleandrin
oleanolic acid
oleic acid
-
-
oligomycin
Omeprazole
-
inhibit mutants G803C, T804C, V805C
orthovanadate
ouabagenin
-
-
Ouabain
p-chloro-diphenyl diselenide
-
i.e. (p-ClC6H4Se)2, activity is restored by DTT
p-methoxyl-diphenyl diselenide
-
i.e. (p-CH3OC6H4Se)2, activity is restored by DTT
palytoxin
Pb2+
-
inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase, overview. Pb2+ inhibits cycling of the enzyme, but it does not affect cytoplasmic Na+ binding and release of Na+ ions at the extracellular side at concentrations below 0.010 mM
PCMB
-
-
perillyl alcohol
polygalacic acid
-
from dried roots of Platycodon grandiflorum
PP1
-
Src kinase inhibitor
prostaglandin E2
-
PGE2
Prostaglandins
-
negative modulator
-
putrescine
-
-
reduced glutathione
-
extracellular and, or intracellular, inhibition of the transport activity of the Na-K pump
rottlerin
-
-
saikosaponin A
-
from dried roots of Bupleurum chinense or Bupleurum scorzonerifolium
sarsasapogenin
-
from dried roots of Anemarrhena asphodeloides
spermidine
-
-
spermine
-
-
strophanthidin
strophantidine
-
-
superoxide
-
-
suramin
-
potent inhibitor, acts on the inside surface of the sodium pump
tetraethylammonium chloride
-
inhibition of pump current is independent on membrane potential
Tumor necrosis factor alpha
-
TNF-alpha, TNF-alpha affects the Na+-K+ pump via PGE2-dependent pathways
-
ursolic acid
-
from whole plant of Prunella vulgaris with dried flowers
vanadate
Yes kinase
-
regulator of the Na+,K+-ATPase activity
-
[Au(2,2'-bipyridine)Cl2]+
-
-
[Au(dimethylsulfoxide)2Cl2]+
-
-
[PdCl(dien)]+
-
noncompetitive, affinity for binding in decreasing order: [PdCl4]2-, [PdCl(dien)]+, [PdCl(Me4dien)]+. Addition of L-cysteine or glutathoione before exposure to Pd(II) complexes prevents inhibition
[PdCl(Me4dien)]+
-
noncompetitive, affinity for binding in decreasing order: [PdCl4]2-, [PdCl(dien)]+, [PdCl(Me4dien)]+. Addition of L-cysteine or glutathoione before exposure to Pd(II) complexes prevents inhibition
-
[PdCl4]2-
-
noncompetitive, affinity for binding in decreasing order: [PdCl4]2-, [PdCl(dien)]+, [PdCl(Me4dien)]+. Addition of L-cysteine or glutathoione before exposure to Pd(II) complexes prevents inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-chloro-2,4-dinitrobenzene
-
increase of the hydrolytic activity of the Na/K ATPase
4-aminobutyric acid
-
hypothesis: stimulate Na+/K+ transport via specific receptor inputs
8-pCPT-CGMP
-
induces a concentration-dependent increase in pump current, activator of PKG
aldosterone
-
control of Na,K-ATPase activity and expression
Baclofen
-
hypothesis: stimulate Na+/K+ transport via specific receptor inputs
beta-catenin
-
expression of beta-catenin significantly enhances the ouabain-sensitive current of the endogeneous Na+/K+-ATPase. beta-Catenin does not significantly modify the retrieval of the Na+/K+ ATPase from the cell membrane. The stimulating effect of beta-catenin on both Na+/K+ ATPase and Na+-coupled glucose transporter SGLT1 activity is observed even in the presence of actinomycin D
-
dephostatin
-
-
desipramine
-
inhibitor of adrenalin uptake and antidepressive action. Treatment differentially enhances the activity of Na+K+-ATPase alpha isoforms
dexamethasone
-
postnatal treatment of lamb with 0.5 mg per kg results in 21.4% increase in Na+K+-ATPase activity and a 30.4% increase in catalytic subunit alpha1 expression in the cerebral cortex. Treatment is not associated with significant changes in subunit beta1 expression. In the kidney, dexamethasone treatment is not associated with significant changes in enzyme activity, or alpha-1- or beta-1-isoform expression
epinephrine
-
hypothesis: stimulate Na+/K+ transport via specific receptor inputs
glyburide
-
presence of 5 mM ATP, slightly stimulating up to 1 mM, inhibitory above. Presence of 0.05 mM ATP, stimulating at least up to 3 mM
Insulin
-
control of Na,K-ATPase activity and expression
-
interleukin-2
-
increases transport activity of the Na/K pump and the content of Na,K-ATPase alpha1 protein
-
K+
-
extracellular
lauric acid
-
presence of 5 mM ATP, inhibitory above 0.25 mM. Presence of 0.05 mM ATP, stimulating up to 0.3 mM, inhibitory above
Lyn kinase
-
Lyn kinase directly binds to the Na+,K+-ATPase alpha3 subunit for regulation of activity
-
monoclonal antibody SSA 78
norepinephrine
-
hypothesis: stimulate Na+/K+ transport via specific receptor inputs
Ouabain
polyclonal antibody pSSA 78
-
-
-
sodium
-
control of Na,K-ATPase activity and expression by intracellular Na+
sodium nitroprusside
-
SNP, NO donor, not a simple concentration-dependent effect on pump current
Vasopressin
-
control of Na,K-ATPase activity and expression
YC-1
-
activator of soluble guanylyl cyclase, sGC
Yes kinase
-
regulator of the Na+,K+-ATPase activity
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00151 - 1.5
ATP
0.7 - 4.6
K+
0.46 - 1.47
K+/out
3.47 - 16.2
Na+
4 - 24.2
Na+/in
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 8
K+
20.1 - 25.5
Na+/in
additional information
additional information
Homo sapiens
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
benzyltriethylammonium chloride
-
half maximum inhibition of Na,K-pump current
0.000157 - 0.0002
orthovanadate
0.0124 - 0.176
Ouabain
1.5
perillyl alcohol
-
pH 7.4, 37C, Na/K-ATPase-enriched A-127 cell extract
0.076
strophanthidin
-
IC50
26.6
tetraethylammonium chloride
-
half maximum inhibition of Na,K-pump current
additional information
additional information
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017
(1R,2R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-2,3a,11,11a-tetrahydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
Homo sapiens
-
pH 7.8, 37C
0.0012
(1R,2R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9-(hydroxymethyl)-15a-methyl-1-(5-oxo-2,5-dihydrofuran-3-yl)-2-(2-oxopropanoyl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
Homo sapiens
-
pH 7.8, 37C
0.0005
(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-13a-formyl-3a,11a-dihydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-11-yl beta-D-glucopyranoside
Homo sapiens
-
pH 7.8, 37C
0.0004
(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9,15a-dimethyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
Homo sapiens
-
pH 7.8, 37C
0.0005
(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9-(hydroxymethyl)-15a-methyl-1-(5-oxo-2,5-dihydrofuran-3-yl)icosahydro-7aH,13aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxine-13a-carbaldehyde
Homo sapiens
-
pH 7.8, 37C
0.0007
1,8,9-trihydroxy-3-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
Rattus norvegicus
-
37C, pH 7.4
0.055
12,20-dihydroxydammar-24-en-3-yl D-glucopyranoside
Sus scrofa
-
-
0.003
2,3-dihydroxy-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-6-one
Rattus norvegicus
-
37C, pH 7.4
0.0007
2,8,9-trihydroxy-3-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
Rattus norvegicus
-
37C, pH 7.4
0.006
2,9-dihydroxy-3,8-dimethoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
Rattus norvegicus
-
37C, pH 7.4
0.003
3,8,9-trihydroxy-2-methoxy-6H-[1]benzofuro[3,2-c]chromen-6-one
Rattus norvegicus
-
37C, pH 7.4
0.171
4,7-diacetoxy-14-hydroxydolast-1(15),8-diene
Cavia porcellus
-
enzym preparation from kidney
0.0007
4-[(1R,3aS,5aS,6aR,7aS,9R,11R,11aS,12aR,13aR,15R,15aS)-3a,11,11a,15-tetrahydroxy-13a-(hydroxymethyl)-9,15a-dimethylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
Homo sapiens
-
pH 7.8, 37C
0.0004
4-[(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15aR)-3a,11,11a-trihydroxy-9,13a-bis(hydroxymethyl)-15a-methylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
Homo sapiens
-
pH 7.8, 37C
0.0003
4-[(1R,3aS,5aS,6aR,7aS,9S,11R,11aS,12aR,13aR,15R,15aS)-3a,11,11a,15-tetrahydroxy-9,13a-bis(hydroxymethyl)-15a-methylicosahydro-1H,7aH-cyclopenta[7,8]phenanthro[2,3-b]pyrano[3,2-e][1,4]dioxin-1-yl]furan-2(5H)-one
Homo sapiens
-
pH 7.8, 37C
0.00009
8-methoxycoumestrol
Homo sapiens
-
isozyme alpha1beta1 Na,K-ATPase, pH 7.4, 30C
0.000575
AuCl4-
Sus scrofa
-
at pH 7.4 and 37C
0.000587
beta-acetyldigoxin
Homo sapiens
-
isozyme alpha1beta1 Na,K-ATPase, pH 7.4, 30C
0.003
bufalin
Homo sapiens
-
-
0.000287
digitoxin
Homo sapiens
-
isozyme alpha1beta1 Na,K-ATPase, pH 7.4, 30C
0.000409
digoxin
Homo sapiens
-
isozyme alpha1beta1 Na,K-ATPase, pH 7.4, 30C
0.031
diphenyl diselenide
Rattus norvegicus
-
pH 7.4, 37C
0.2
epicatechin
Sus scrofa
-
pH 7.0, 37C
0.0008
epicatechin-3-gallate
Sus scrofa
-
pH 7.0, 37C
0.001
epigallocatechin-3-gallate
Sus scrofa
-
pH 7.0, 37C
0.006
m-trifluoromethyl-diphenyl diselenide
Rattus norvegicus
-
pH 7.4, 37C
0.000282
methyldigoxin
Homo sapiens
-
isozyme alpha1beta1 Na,K-ATPase, pH 7.4, 30C
0.0008 - 0.012
Mg2+
0.0012
odoroside A
Homo sapiens
-
pH 7.0, 37C
0.115
oleic acid
Cavia porcellus
-
enzym preparation from kidney
0.0001 - 0.4
Ouabain
0.006
p-chloro-diphenyl diselenide
Rattus norvegicus
-
pH 7.4, 37C
0.045
p-methoxyl-diphenyl diselenide
Rattus norvegicus
-
pH 7.4, 37C
0.0398
[Au(2,2'-bipyridine)Cl2]+
Sus scrofa
-
at pH 7.4 and 37C
0.0055
[Au(dimethylsulfoxide)2Cl2]+
Sus scrofa
-
at pH 7.4 and 37C
0.0121
[PdCl(dien)]+
Sus scrofa
-
pH 7.4, 37C
0.0236
[PdCl(Me4dien)]+
Sus scrofa
-
pH 7.4, 37C
-
0.0225
[PdCl4]2-
Sus scrofa
-
pH 7.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.085
-
high salt diet
0.133
-
enzyme activity in HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication
0.165
-
high normal diet
0.183
-
enzyme activity in HK-2 cells, pH not specified in the publication, temperature not specified in the publication
0.25
-
enzyme activity in ouabain-pretreated HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication
0.267
-
enzyme activity in ouabain-pretreated HK-2 cells, pH not specified in the publication, temperature not specified in the publication
11.96
-
-
19.84
-
in reconstituted vesicles
30
-
at pH 7.0 and 37C
42
-
with p-nitrophenyl phosphate as substrate
120
-
mutant K691A
175
-
mutant D714A
195
-
mutant K691R
259.1
-
with p-nitrophenolphosphate as substrate
575
-
mutant D714E
686.1
-
with ATP as substrate
700
-
with ATP as substrate
1105
-
wild-type
additional information
-
decrease in specific activity by 78.5% in neonatal hypothyroidism
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
7 - 7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 7.8
-
variation of extracelluar pH for measurements of Na+-K+ pump activity
7
-
more than 90% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
23
-
myocytes in superfusion chamber
30
-
assay at
32
-
cells in temperature-controlled Lucite bath for patch-clamp experiments
additional information
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
glioblastoma cells, A172 cells overexpress Na/K-ATPase alpha1 isoform in the caveolar structure
Manually annotated by BRENDA team
-
male and female
Manually annotated by BRENDA team
-
isolated mucosal cells of
Manually annotated by BRENDA team
-
inner ear
Manually annotated by BRENDA team
-
epithelium
Manually annotated by BRENDA team
-
red portion of the muscle
Manually annotated by BRENDA team
-
of the input layer of cerebellar cortex
Manually annotated by BRENDA team
-
proximal tubule cell line
Manually annotated by BRENDA team
-
muscle cell line, A-769662 inhibits the Na+-K+-ATPase cell surface abundance in L6 cells by decreasing the phosphorylation of the alpha-subunit
Manually annotated by BRENDA team
-
at fifth instar feeding stage
Manually annotated by BRENDA team
-
ventricular
Manually annotated by BRENDA team
-
enzyme activity is well-maintained in the dissociated neostriatal neurons
Manually annotated by BRENDA team
-
presence of Na+/K+-ATPAse
Manually annotated by BRENDA team
-
a pancreatic cancer cell line
Manually annotated by BRENDA team
-
a pancreatic cancer cell line
Manually annotated by BRENDA team
-
differential distribution of enzyme alpha subunit isoforms in subicular interneurones and pyramidal cells. While ATP1A3-isoforms regulate sodium and potassium homeostasis in subicular interneurones, ATPA1-isoforms assume this function in pyramidal cells
Manually annotated by BRENDA team
-
white muscle
Manually annotated by BRENDA team
additional information