Information on EC 3.6.3.47 - fatty-acyl-CoA-transporting ATPase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.3.47
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RECOMMENDED NAME
GeneOntology No.
fatty-acyl-CoA-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + fatty acyl CoA/cis = ADP + phosphate + fatty acyl CoA/trans
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
transmembrane transport
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (fatty-acyl-CoA-transporting)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. An animal and yeast enzyme that transports fatty acyl CoA into and out of peroxisomes. In humans, it is associated with Zellweger's syndrome.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain BJ1991, gene ABCD1
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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germination of ped3 mutants is significantly impaired, suggesting that PED3 regulates dormancy and germination. A ped3 abi5 (ABA insensitive 5) double mutant does not show any of the expression patterns of ped3 mutants and rescues the impaired germination phenotype of the ped3 mutant. The impaired germination of ped3 can also be rescued by removal of pectin from the seed coat using exogenous polygalacturonase or acidic conditions
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + fatty acyl CoA/cis
ADP + phosphate + fatty acyl CoA/trans
show the reaction diagram
ATP + H2O + fatty acyl-CoA/cis
ADP + phosphate + fatty acyl-CoA/trans
show the reaction diagram
ATP + H2O + heptadecanoyl-CoA/cis
ADP + phosphate + heptadecanoyl-CoA/trans
show the reaction diagram
ATP + H2O + long-chain fatty acyl CoA/cis
ADP + phosphate + long-chain fatty acyl CoA/trans
show the reaction diagram
-
-
-
?
C18:1-CoA/out + ATP + H2O
C18:1-CoA/in + ADP + phosphate
show the reaction diagram
cerotic acid + ATP + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + fatty acyl CoA/cis
ADP + phosphate + fatty acyl CoA/trans
show the reaction diagram
ATP + H2O + fatty acyl-CoA/cis
ADP + phosphate + fatty acyl-CoA/trans
show the reaction diagram
ATP + H2O + long-chain fatty acyl CoA/cis
ADP + phosphate + long-chain fatty acyl CoA/trans
show the reaction diagram
Q7Z9L6
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aluminium fluoride
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strong inhibition
siRNA
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RNAi technique is used to knockdown the ABCD3 gene encoding PMP70
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additional information
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the ATPase activity of ALDP-His is insensitive to oligomycin, azide and vanadate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EGFP tag
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1.9fold increase of activity
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Pex19p
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protein required for the peroxisomal membrane synthesis, binds to PMP70 co-translationally and keeps PMP70 in a proper conformation for the localization to peroxisome, PMP70 forms 75% aggregates during translation in the absence of Pex19p
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0743
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ALDRP-His, membrane fractions (about 10 mg of membrane protein) are incubated at 37°C in 0.1 ml of 40 mM Tris-HCl (pH 7.4) containing 0.1 mM EGTA, 1 mM dithiothreitol, 2mM ouabain and 15 mM MgSO4, and 5 mM NaATP
0.0774
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ALDP-His, membrane fractions (about 10 mg of membrane protein) are incubated at 37°C in 0.1 ml of 40 mM Tris-HCl (pH 7.4) containing 0.1 mM EGTA, 1 mM dithiothreitol, 2mM ouabain and 15 mM MgSO4, and 5 mM NaATP
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
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C6 glial cell
Manually annotated by BRENDA team
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SV40-transformed, from a patient with X-linked adrenoleukodystrophy missense mutation A626T
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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when the N-terminal 80 amino acid residue (N80)-segment preceding transmembrane segment (TM) 1 is deleted and the TM1-TM2 region is fused to EGFP, the TM1 segment induces endoplasmic reticulum-targeting
Manually annotated by BRENDA team
integral membrane protein
Manually annotated by BRENDA team
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when the N-terminal 80-segment is fused to EGFP, the fusion protein is targeted to the outer mitochondrial membrane
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
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ALDRP
59753
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ALDP, x * 59753, electrospray ionization mass spectrometry
75483
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PMP70, x * 75483, electrospray ionization mass spectrometry
80000
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ALDP-His, SDS-PAGE
84000
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x * 84000, calculation from amino acid sequence
87000
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ALDRP-EGFP fusion protein
149400
x * 149400, deduced from gene sequence and SDS-PAGE
300000 - 400000
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PMP70, ALDP and ALDPR are found as large protein complexes upon sucrose density gradient centrifugation, since the sedimentation properties of a solubilized membrane protein also depend on its partial specific volume, shape and amount and type of protein-bound detergents/membrane lipids, no conclusions about regarding the true size of the observed protein complexes can be inferred
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
homodimer
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ALDP, preparative immunoprecipitation, PMP70, incubation in ATP-agarose and sucrose gradient centrifugation
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ALDP-His, immobilized metal affinity chromatography
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ATP-binding domain
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Spodoptera frugiperda strain 21 cells
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gene ABC1, DNA and amino acid sequence determination and analysis; gene ABC2, DNA and amino acid sequence determination and analysis
gene ABCD1, DNA and amino oacid sequence determination and analysis
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into pECFP-N1 and pEYFP-N1 vectors for transfection of VERO cells; into pECFP-N1, pEYFP-N1, or pECFP-C1 vectors for transfection of VERO cells
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into the pMAM2-BSD, pEGFPN-1 and pcDNA4HisMax TOP vector for transfection of CHO cells, and as templates for the construction of mutant cDNAs
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transfection of cDNA of rat PMP70 into Chineses hamster ovary cells and establishing of cell lines stably expressing PMP70
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using transient transfection of COS cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K487A
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mutation in Walker A motif, no beta-oxidation activity
ALDP390
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mutant, comprises the amino acids 1 to 390
ALDP550
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mutant, comprises the amino acids 1 to 550
ALDP658
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mutant, comprises the amino acids 1 to 658
ALDPDELTA551-657
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mutant, residues 551-657 are deleted
ALDPDELTANBF
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mutant, residues 391-657 are deleted
G116R
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naturally occuring missense mutation
H667D
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naturally occuring missense mutation
Q544R
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naturally occuring missense mutation
R104C
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naturally occuring missense mutation
R617H
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naturally occuring missense mutation
S342P
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naturally occuring missense mutation
S606L
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naturally occuring missense mutation
S606P
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naturally occuring missense mutation
Y174C
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naturally occuring missense mutation
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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