Information on EC 3.6.3.43 - peptide-transporting ATPase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.6.3.43
-
RECOMMENDED NAME
GeneOntology No.
peptide-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + peptide/in = ADP + phosphate + peptide/out
show the reaction diagram
ABC-type (ATP-binding cassette) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A family of enzymes which export alpha-hemolysin, cyclolysin, colicin V and sidophores from Gram-negative bacteria, and bacteriocin, subtilin, competence factor and pediocin from Gram-positive bacteria
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydolysis of phosphoric ester
-
-
-
-
hydrolysis of phosphoric ester
-
-
transmembrane transport
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (peptide-exporting)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A family of enzymes that exports alpha-hemolysin, cyclolysin, colicin V and siderophores from Gram-negative bacteria, and bacteriocin, subtilin, competence factor and pediocin from Gram-positive bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
strain 168
-
-
Manually annotated by BRENDA team
genes haf-4 and haf-9
-
-
Manually annotated by BRENDA team
isoform SLC15A1b
UniProt
Manually annotated by BRENDA team
Gram-negative bacteria
-
-
-
Manually annotated by BRENDA team
strain LMG 2081
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
recombinant protein expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme ablation results in 3-5fold reduction in the in vivo rate and extent of valacyclovir absorption
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O + peptide/in
AMP + phosphate + peptide/out
show the reaction diagram
ATP + H2O + 5'-Lphenylalanyl-L-glycyl-floxuridine/in
ADP + phosphate + 5'-Lphenylalanyl-L-glycyl-floxuridine/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + aerolysin/in
ADP + phosphate + aerolysin/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + alpha-haemolysin/in
ADP + phosphate + alpha-haemolysin/out
show the reaction diagram
ATP + H2O + anserine/out
ADP + phosphate + anserine/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/in
ADP + phosphate + beta-L-Ala-L-Lys-7-amido-4-methylcoumarin-3-acetic acid/out
show the reaction diagram
-
-
-
?
ATP + H2O + carnosine/out
ADP + phosphate + carnosine/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + cefadroxil/in
ADP + phosphate + cefadroxil/out
show the reaction diagram
ATP + H2O + CRYQKSTEL/in
ADP + phosphate + CRYQKSTEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + D-phenylglycine-L-Dopa/in
ADP + phosphate + D-phenylglycine-L-Dopa/out
show the reaction diagram
ATP + H2O + delta-aminolevulinic acid/in
ADP + phosphate + delta-aminolevulinic acid/out
show the reaction diagram
ATP + H2O + EPGNTWDED/out
ADP + phosphate + EPGNTWDED/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + formyl-methionyl-leucine-phenylalanine/in
ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
ATP + H2O + Gly-Gly/in
ADP + phosphate + Gly-Gly/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + Gly-L-His-Gly/in
ADP + phosphate + Gly-L-His-Gly/out
show the reaction diagram
-
isoform PTR6
-
-
?
ATP + H2O + Gly-L-His/in
ADP + phosphate + Gly-L-His/out
show the reaction diagram
-
isoform PTR6
-
-
?
ATP + H2O + glycyl-sarcosine/in
ADP + phosphate + glycyl-sarcosine/out
show the reaction diagram
ATP + H2O + glycylsarcosine/out
ADP + phosphate + glycylsarcosine/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + guanidine oseltamivir carboxylate-L-Val/in
ADP + phosphate + guanidine oseltamivir carboxylate-L-Val/out
show the reaction diagram
ATP + H2O + hemoprotein HasA/in
ADP + phosphate + hemoprotein HasA/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + JBP485/in
ADP + phosphate + JBP485/out
show the reaction diagram
ATP + H2O + L-Ala-gamma-D-Glu-meso-diaminopimelic acid/in
ADP + phosphate + L-Ala-gamma-D-Glu-meso-diaminopimelic acid/out
show the reaction diagram
ATP + H2O + L-Ala-L-Ala/in
ADP + phosphate + L-Ala-L-Ala/out
show the reaction diagram
ATP + H2O + L-Ala-L-Asp/in
ADP + phosphate + L-Ala-L-Asp/out
show the reaction diagram
ATP + H2O + L-Ala-L-Lys/in
ADP + phosphate + L-Ala-L-Lys/out
show the reaction diagram
ATP + H2O + L-Val-didanosine/in
ADP + phosphate + L-Val-didanosine/out
show the reaction diagram
ATP + H2O + lacterococcin G/in
ADP + phosphate + lacterococcin G/out
show the reaction diagram
ATP + H2O + lacterococcin/in
ADP + phosphate + lacterococcin/out
show the reaction diagram
-
lactococcal bacteriocin
-
-
?
ATP + H2O + lacticin 481/in
ADP + phosphate + lacticin 481/out
show the reaction diagram
-
lactococcal bacteriocin
-
-
?
ATP + H2O + major histocompatibility complex class I molecules/in
ADP + phosphate + major histocompatibility complex class I molecules/out
show the reaction diagram
ATP + H2O + metalloprotease/in
ADP + phosphate + metalloprotease/out
show the reaction diagram
-
metalloproteases PrtA,B,C,D
-
-
?
ATP + H2O + midodrine/in
ADP + phosphate + midodrine/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + muramyl dipeptide/in
ADP + phosphate + muramyl dipeptide/out
show the reaction diagram
ATP + H2O + NP-647/in
ADP + phosphate + NP-647/out
show the reaction diagram
ATP + H2O + oseltamivir carboxylate/in
ADP + phosphate + oseltamivir carboxylate/out
show the reaction diagram
ATP + H2O + oseltamivir/in
ADP + phosphate + oseltamivir/out
show the reaction diagram
ATP + H2O + peptide/in
ADP + phosphate + peptide/out
show the reaction diagram
ATP + H2O + peptide/out
ADP + phosphate + peptide/in
show the reaction diagram
-
ATP best substrate
-
-
?
ATP + H2O + protein/in
ADP + phosphate + protein/out
show the reaction diagram
Gram-negative bacteria
-
-
-
-
?
ATP + H2O + protein/out
ADP + phosphate + protein/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + RCYQKSTEL/in
ADP + phosphate + RCYQKSTEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRCQKSTEL/in
ADP + phosphate + RRCQKSTEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYCKSTEL/in
ADP + phosphate + RRYCKSTEL/out
show the reaction diagram
ATP + H2O + RRYNASTEL/out
ADP + phosphate + RRYNASTEL/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + RRYQCSTEL/in
ADP + phosphate + RRYQCSTEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYQKCTEL/in
ADP + phosphate + RRYQKCTEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYQKSCEL/in
ADP + phosphate + RRYQKSCEL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYQKSTCL/in
ADP + phosphate + RRYQKSTCL/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYQKSTEC/in
ADP + phosphate + RRYQKSTEC/out
show the reaction diagram
-
peptide transport assay, fluorescein is coupled via the cysteine
-
-
?
ATP + H2O + RRYQKSTEL/out
ADP + phosphate + RRYQKSTEL/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + RRYQNSTCL/in
ADP + phosphate + RRYQNSTCL/out
show the reaction diagram
ATP + H2O + subtilisin/in
ADP + phosphate + subtilisin/out
show the reaction diagram
ATP + H2O + TVDNKTRYR/in
ADP + phosphate + TVDNKTRYR/out
show the reaction diagram
-
substrate in peptide transport assay, peptide is biotin-labeled at the lysine residue
-
-
?
ATP + H2O + valacyclovir/in
ADP + phosphate + valacyclovir/out
show the reaction diagram
ATP + H2O + valganciclovir/in
ADP + phosphate + valganciclovir /out
show the reaction diagram
-
-
-
-
?
ATP + H2O + Zan-L-Val/in
ADP + phosphate + Zan-L-Val/out
show the reaction diagram
CTP + H2O + peptide/out
CDP + phosphate + peptide/in
show the reaction diagram
-
affinity for CTP is about 30fold lower compared to ATP
-
-
?
GTP + H2O + peptide/in
GDP + phosphate + peptide/out
show the reaction diagram
GTP + H2O + peptide/out
GDP + phosphate + peptide/in
show the reaction diagram
-
affinity for GTP is about 30fold lower compared to ATP
-
-
?
UTP + H2O + peptide/out
UDP + phosphate + peptide/in
show the reaction diagram
-
affinity for UTP is about 30fold lower compared to ATP
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + aerolysin/in
ADP + phosphate + aerolysin/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + alpha-haemolysin/in
ADP + phosphate + alpha-haemolysin/out
show the reaction diagram
ATP + H2O + formyl-methionyl-leucine-phenylalanine/in
ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
ATP + H2O + hemoprotein HasA/in
ADP + phosphate + hemoprotein HasA/out
show the reaction diagram
-
-
-
-
?
ATP + H2O + L-Ala-gamma-D-Glu-meso-diaminopimelic acid/in
ADP + phosphate + L-Ala-gamma-D-Glu-meso-diaminopimelic acid/out
show the reaction diagram
ATP + H2O + lacterococcin G/in
ADP + phosphate + lacterococcin G/out
show the reaction diagram
ATP + H2O + lacterococcin/in
ADP + phosphate + lacterococcin/out
show the reaction diagram
-
lactococcal bacteriocin
-
-
?
ATP + H2O + lacticin 481/in
ADP + phosphate + lacticin 481/out
show the reaction diagram
-
lactococcal bacteriocin
-
-
?
ATP + H2O + major histocompatibility complex class I molecules/in
ADP + phosphate + major histocompatibility complex class I molecules/out
show the reaction diagram
ATP + H2O + metalloprotease/in
ADP + phosphate + metalloprotease/out
show the reaction diagram
-
metalloproteases PrtA,B,C,D
-
-
?
ATP + H2O + muramyl dipeptide/in
ADP + phosphate + muramyl dipeptide/out
show the reaction diagram
ATP + H2O + peptide/in
ADP + phosphate + peptide/out
show the reaction diagram
ATP + H2O + protein/in
ADP + phosphate + protein/out
show the reaction diagram
Gram-negative bacteria
-
-
-
-
?
ATP + H2O + subtilisin/in
ADP + phosphate + subtilisin/out
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
divalent cation required, Mg2+ shows 40% of the activity with Mn2+
Mn2+
-
divalent cation required, Mn2+ is most effective
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-biphenyl-L-alanyl-L-arginine methyl ester
-
peptide derived from lactoferricin, at 1 mM, 10% inhibition of glycylsarcosine-induced current
4-(gamma-aminobutanoyl)-Lys
-
anserine
beta-Ala-Lys
-
Carbonyl cyanide m-chlorophenylhydrazone
10 mM, 20% residual activity; 10 mM, 26% residual activity
carnosine
cefaclor
cisplatin
-
IC50 value for growth inhibition of cell line PC-3 0.0033 mM, DU-145 0.0045 mM, 2008 0.0194 mM, C-13 0.117 mM, L-540 0.0025 mM
diethyl dicarbonate
10 mM, 0.4% residual activity; 10 mM, 0.6% residual activity
Fv fragment of the monoclonal antibody mAb148.3
-
Fv binding to the C terminus of TAP1
-
ICP47
-
herpes simplex virus protein
-
KCl
-
no ATPase activity above 300 mM
L-Lys-L-Lys
L-Phe-L-Ala
in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala; in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe
L-Phe-L-Phe
in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe. Phe-Ala reduces leak currents by binding to the substrate-binding site with a high apparent affinity
L-Trp-L-Ala
in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe
N-ethylmaleimide
10 mM, 12% residual activity; 10 mM, 14% residual activity
NaCl
-
no ATPase activity above 300 mM
orthovanadate
RWR-NH(benzyl)
-
peptide derived from lactoferricin, at 1 mM, 87% inhibition of glycylsarcosine-induced current, peptide can also impair membrane protein functions in an unspecific manner
RWR-NH2
-
peptide derived from lactoferricin, at 1 mM, 15% inhibition of glycylsarcosine-induced current
ubiquitin-like specific protease 18
-
-
-
[AuIIIBr2(dtc-Sar-Aib-O(t-Bu))]
-
IC50 value for growth inhibition of cell line PC-3 0.0008 mM, DU-145 0.0014 mM, 2008 0.0045 mM, C-13 0.0037 mM, L-540 0.0015 mM
[AuIIICl2(dtc-Sar-Aib-O(t-Bu))]
-
IC50 value for growth inhibition of cell line PC-3 0.0011 mM, DU-145 0.0022 mM, 2008 0.0047 mM, C-13 0.0051 mM, L-540 0.0017 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.174 - 2.33
ATP
0.000574
RRYNASTEL
-
pH 7.4, 32°C
0.000161
RRYQKSTEL
-
pH 7.4, 32°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0005 - 25
ATP
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.96
alafosfalin
0.033 - 1.55
anserine
0.08 - 0.36
L-Ala-L-Ala
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 1.5
alafosfalin
0.12 - 0.56
L-Ala-L-Ala
0.016
orthovanadate
Escherichia coli
-
IC50: 0.016 mM, competitive
2.7
RWR-NH2
Homo sapiens
-
pH 7.4, 22°C
additional information
beta-Ala-Lys
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000022
-
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP; peptide transport rate, wild type, in the presence of 3 mM ATP and 1 mM orthovanadate
0.00000026
-
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL; peptide transport rate, wild type, in the presence of 3 mM AMP
0.0000003
-
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 1 mM orthovanadate
0.00000035
-
peptide transport rate, wild type, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL
0.00000039
-
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM AMP
0.00000156
-
peptide transport rate, wild type, in the presence of 3 mM ATP
0.000003
-
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-eggPG, 9 to 1
0.000004
-
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPE, 9 to 1
0.000006
-
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine heart lipids
0.000011
-
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine brain lipids
0.000023
-
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine liver lipids
0.000028
-
peptide transport rate
0.000045
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 100% phosphatidylcholine
0.00007
-
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 90% DOPS
0.000075
-
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPA, 9 to 1
0.000095
-
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 10% DOPS
0.000112
-
peptide transport rate
0.000115
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 70% phosphatidylcholine
0.000135
-
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPS, 9 to 1
0.000245
-
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 70% DOPS
0.00026
-
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 30% DOPS
0.00028
-
peptide transport rate
0.000295
-
peptide transport rate
0.00042
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids
0.000423
-
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 50% DOPS
0.000477
-
peptide transport rate
0.000505
-
peptide transport rate, TAPL reconstitution in Eschericha coli liposomes, for comparison
0.000533
-
peptide transport rate
0.000535
-
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, for comparison in Eschericha coli liposomes
0.00055
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, no phosphatidylcholine
0.000673
-
peptide transport rate
0.000715
-
peptide transport rate
0.00127
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 50% phosphatidylcholine
0.00129
-
peptide transport rate
0.00132
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 10% phosphatidylcholine
0.00168
-
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 30% phosphatidylcholine
1.93
-
pH 7.4, 32°C, substrate RRYNASTEL
2.02
-
pH 7.4, 32°C, substrate RRYQKSTEL
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
mutants E388D, E388Q
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
mutant Q701H
6 - 7
-
mutant D668/Q701H
7 - 8.5
-
pH 7.0: about 75% of maximal activity, pH 8.5: about 85% of maximal activity
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
-
peptide transport assay
37
-
peptide transport assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
TAP1-deficient skin fibroblast cell line
Manually annotated by BRENDA team
-
Burkitt lymphoma cell, i.e. Raji cell
Manually annotated by BRENDA team
-
TAPL expression is strongly induced during differentiation of monocytes to dendritic cells and to macrophages
Manually annotated by BRENDA team
-
cisplatin resistant cell
Manually annotated by BRENDA team
-
in grain-filling seeds, expression of isoforms OsPTR4, OsPTR7 and OsPTR8 increases from early to late stages of seed development and that of OsPTR2, OsPTR3 and OsPTR6 decreases
Manually annotated by BRENDA team
-
TAP2-deficient skin fibroblast cell line
Manually annotated by BRENDA team
-
human TAP-deficient lymphoblastoid cell, expressing rat wild-type TAP or single rat TAP1 or TAP2 chains
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17100
2 * 17100, isoform PEPT1, X-ray crystallography
20000
-
TMD0 subunit
20100
2 * 20100, isoform PEPT2, X-ray crystallography
22300
-
SpaG, nucleotide sequence
28400
-
LctG, nucleotide sequence
29000
-
nucleotide-binding domain of TAP1, determined by SDS-PAGE
29200
-
LctE, nucleotide sequence
34000
-
2 * 34000, SDS-PAGE
34400
-
LctF, nucleotide sequence
50200
-
SpaF, nucleotide sequence
60000
-
TAPL core complex
66000
-
gel filtration in presence of MgATP2- and BeFx or orthovanadate
80000
-
HylB, nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
homodimer
monomer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homology modeling based on the crystal structure of the Shewanella oneidensis peptide transporter PepTso, identifies Glu56 and Arg305 as potential periplasmic gating residues
recombinant cytosolic ATP-binding domain, N-terminal His-tag
-
recombinant nucleotide-binding domain
-
EPR spectroscopy using covalently attached 2,2,5,5-tetramethylpyrrolidine-1-oxyl spin probes, and 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid spin-labeled peptides. The side-chains' mobility is strongly restricted at the ends of the peptide, whereas the central region is flexible, suggesting a central peptide bulge. Peptides bind to TAP in an extended kinked structure, analogous to those bound to MHC class I proteins
-
extracellular domain from isoform PepT1, sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 6000, 0.1 M MES (pH 6.0), and 0.2 M ammonium chloride
extracellular domain from isoform PepT2, sitting drop vapor diffusion method, using 0.2 M (NH4)3 citrate (pH 5.8) and 21% PEG (w/v) 3350
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, the ATPase domain is indefinitly stable in phosphate or Tris buffer, pH 8, in the absence of ATP concentrations of up to 25 mg/ml
-
4°C, the ATPase domain is stable for at least 2 days in phosphate or Tris buffer, pH 8, in the absence of ATP concentrations of up to 25 mg/ml
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crude membranes containing TAPL are prepared from Sf9 insect cells, furthermore a SP Sepharose Fast Low column and a Zn2+-iminodiacetate column are used
-
mutant and wild-type enzymes partially by membrane fractionation
-
Ni–NTA agarose column chromatography, and gel filtration
-
recombinant cytosolic ATP-binding domain, N-terminal His-tag
-
recombinant nucleotide-binding domain
-
recombinant protein expressed in Escherichia coli
-
recombinant TAPL from membranes of Drosophila melanogaster S2 cells by detergent solubilization, and ADP or ATP affinity chromatography, not by AMP affinity
using a Zn2+-iminodiacetic acid column and a Superdex 200HR 26/60 column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a set of N-terminally truncated human TAP variants are generated, PCR products are cloned into the vector pFASTBac Dual for expression in the Baculovirus system
-
baculoviruses encoding a histidine-tagged and a green fluorescent protein tagged version of TAP1, and a yellow fluorescent protein-tagged version of TAP2 are obtained
-
exeA, subcloned into pEI14b, expressed in Escherichia coli
-
expressed in Chinese hamster ovary cells
-
expressed in Escherichia coli
-
expressed in HeLa cells; expressed in MEF cells
-
expressed in Nicotiana tabacum protoplasts and leaf epidermal cells
-
expressed in Xenopus laevis oocytes
-
expression in Caco-2 cells and HeLa cells
-
expression in Pichia pastoris
-
expression in spontaneous hypertensive rat kidney proximal tubule cells
-
expression in Xenopus laevis and Saccharomyces cerevisiae; expression in Xenopus laevis and Saccharomyces cerevisiae
expression in Xenopus laevis oocyte; expression in Xenopus laevis oocyte
expression in Xenopus laevis oocytes
-
genes haf-4 and haf-5, phylogenetic tree, overexpression of wild-type and mutant GFP-tagged enzymes
-
human TAPL is cloned into pEGFP-N3 and pIRES2-EGFP resulting in TAPL with a C-terminal EGFP and TAPL co-translated EGFP, respectively, TAPL containing a C-terminal myc-tag is cloned into pcDNA3.1+, and into the retroviral vector pLPCX
-
into the expression vector pEGFP1-N1 for transfection of MC4 cells
-
into the pSP64 vector
-
isozyme 12A, expression of TAPL in membranes of Drosophila melanogaster S2 cells
lctF, lctE, lctG and deletion mutants
-
LncC-PhoA chimeras, expressed by Escherichia coli
-
phylogenetic tree
-
recombinant baculovirus, encoding human TAP1 and TAP2, is used for infection of Sf9 cells, used for the peptide transport assay
-
subtilin gene
-
TAPL containing a C-terminal His10 tag or a Strep-tag II is cloned for expression in Sf9 insect cells infected with recombinant baculovirus
-
the coding sequence of the nucleotide-binding domain of TAP1, amino acids 489-748, is cloned into the pET21a+ vector
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
high expression during senescence
peptide transporter 1 mRNA expressions is increased at 5-7 days post hatching
short-term fasting results in up-regulation of PepT1 mRNA levels, while prolonged fasting results in down-regulation. The resumption of feeding results in up-regulation of PepT1 above pre-fasted levels
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G55A
-
mutation in the potential ATP-binding site in ExeA, decreased rate of aerolysin secretion
G55D
-
mutation in the potential ATP-binding site in ExeA, decreased rate of aerolysin secretion
G55V
-
mutation in the potential ATP-binding site in ExeA, decreased rate of aerolysin secretion
K539M
-
introduction of a Walker A mutation in the haf-4 construct. The mutation in ATP-binding site of HAF-4 inhibits the maturation of granules as dominant-negative effects. The inactive form of HAF-4 does not rescue the phenotype
A123V
partial loss of uptake
A264P
partial loss of uptake
A285V
complete loss of uptake
A303G
partial loss of uptake
A68P
complete loss of uptake
D630M
-
ATPase activity is 0.6% of wild-type activity
E20D
mutant is not affected by the bulk pH in the range tested, no dramatic change in IC50 value for peptides Gly-Lys, beta-Ala-Lys
E20Q
mutant is not affected by the bulk pH in the range tested, no dramatic change in IC50 value for peptides Gly-Lys, beta-Ala-Lys
E388D
increase in IC50 value for peptides Gly-Lys, beta-Ala-Lys, increase in pH-optimum
E388Q
increase in IC50 value for peptides Gly-Lys, beta-Ala-Lys, increase in pH-optimum
E56G
complete loss of uptake
F197I
change in selectivity
F289L
complete loss of uptake
F289S
complete loss of uptake
F301I
complete loss of uptake
G101D
partial loss of uptake
G127D
partial loss of uptake
G78C
complete loss of uptake
G86R
partial loss of uptake
H662A
-
inactive
I100V
no membrane localization
I122N
partial loss of uptake
I60N
complete loss of uptake
K274I
change in selectivity
K508M
-
ATPase activity is 1.3% of wild-type activity
L136R
complete loss of uptake
L137H
no membrane localization
L190V
change in selectivity
L324V
change in selectivity
L98R
complete loss of uptake
M154K
change in selectivity
M295K
complete loss of uptake
N196K
partial loss of uptake
N300I
complete loss of uptake
N300Y
partial loss of uptake
N306I
partial loss of uptake
P326Q
partial loss of uptake
P624C
-
insoluble mutant protein
P624L
-
insoluble mutant protein
P624R
-
insoluble mutant protein
P624S
-
insoluble mutant protein
Q320L
complete loss of uptake
R305C
partial loss of uptake
S59P
complete loss of uptake
T297A
complete loss of uptake
V252E
change in selectivity
V548A
-
insoluble mutant protein
Y477W
-
KM-value for ATP is 1.75fold higher than wild-type value. kcat for ATP is 4.3fold higher than wild-type value
D630M
-
ATPase activity is 0.6% of wild-type activity
-
K508M
-
ATPase activity is 1.3% of wild-type activity
-
P624L
-
insoluble mutant protein
-
P624S
-
insoluble mutant protein
-
V548A
-
insoluble mutant protein
-
coreTAP1
-
mutant, delta2-122
coreTAP2
-
mutant, delta2-122
D668E
-
mutation in the nucleotide-binding domain of TAP1
D668E/Q701H
-
TAP1 mutant
D668E/Q710H
-
mutation in the nucleotide-binding domain of TAP1
D668N
-
TAP1 mutant
D668N/Q701A
-
TAP1 mutant
DELTAN1-2TAP1
-
mutant, delta2-89
DELTAN1-2TAP2
-
mutant, delta2-44
DELTAN1-3TAP1
-
mutant, delta2-132
DELTAN1TAP1
-
mutant, delta2-44
DELTAN1TAP2
-
mutant, delta2-44
E632D
-
TAP2 mutant
E632D/H661Q
-
TAP2 mutant
E632Q
-
TAP2 mutant
E632Q/H661A
-
TAP2 mutant
E632Q/H662A
-
TAP2 mutant
H661Q
-
TAP2 mutant
K545A/H699A
-
TAPL double mutant
Q710H
-
mutation in the nucleotide-binding domain of TAP1
TAP2DELTATM1
-
mutant, amino acids 2-42 are removed
TAP2DELTATM2
-
mutant, amino acids 2-88 are removed
TAP2DELTATM3
-
mutant, amino acids 2-128 are removed
TAP2DELTATM4
-
mutant, amino acids 2-185 are removed
TAP2DELTATM5
-
mutant, amino acids 2-241 are removed
E599Q
-
3000fold reduction of ATPase activity compared to wild-type
additional information
Show AA Sequence (358 entries)
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