Information on EC 3.6.3.34 - iron-chelate-transporting ATPase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.6.3.34
-
RECOMMENDED NAME
GeneOntology No.
iron-chelate-transporting ATPase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + iron chelate/out = ADP + phosphate + iron chelate/in
show the reaction diagram
the FhuC protein contains two ATP-binding domains
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ATP + H2O + iron chelate/out = ADP + phosphate + iron chelate/in
show the reaction diagram
the periplasmic FhuD interacts with a transmembrane segment and a cytoplasmic loop of the integral FhuB
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ATP + H2O + iron chelate/out = ADP + phosphate + iron chelate/in
show the reaction diagram
ABC-type (ATP-binding cassette) ATPase, characterised by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports Fe-enterobactin, Fe-dicitrate, Fe-hydoxymate and other siderophores
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
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transmembrane transport
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-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (iron-chelate-importing)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports Fe-enterobactin, Fe-dicitrate, Fe-hydroxamate and other siderophores.
CAS REGISTRY NUMBER
COMMENTARY
9000-83-3
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + albomycin/out
ADP + phosphate + albomycin/in
show the reaction diagram
-
-
-
?
ATP + H2O + desferrioxamine/out
ADP + phosphate + desferrioxamine/in
show the reaction diagram
-
-
-
?
ATP + H2O + enterobactin/out
ADP + phosphate + enterobactin/in
show the reaction diagram
-
fep-transport system
-
?
ATP + H2O + ferric dicitrate/out
ADP + phosphate + ferric dicitrate/in
show the reaction diagram
-
fec-transport system
-
?
ATP + H2O + ferrichrome/out
ADP + phosphate + ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + ferrioxamine B/out
ADP + phosphate + ferrioxamine B/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
fhu-transport system
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?
ATP + H2O + rhodotorulic acid/out
ADP + phosphate + rhodotorulic acid/in
show the reaction diagram
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-
-
?
ATP + H2O + salmycin/out
ADP + phosphate + salmycin/in
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + desferrioxamine/out
ADP + phosphate + desferrioxamine/in
show the reaction diagram
-
-
-
?
ATP + H2O + enterobactin/out
ADP + phosphate + enterobactin/in
show the reaction diagram
-
fep-transport system
-
?
ATP + H2O + ferric dicitrate/out
ADP + phosphate + ferric dicitrate/in
show the reaction diagram
-
fec-transport system
-
?
ATP + H2O + ferrichrome/out
ADP + phosphate + ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + ferrioxamine B/out
ADP + phosphate + ferrioxamine B/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
-
-
?
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
-
fhu-transport system
-
?
ATP + H2O + rhodotorulic acid/out
ADP + phosphate + rhodotorulic acid/in
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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cross-inhibition of ferric hydroxamates to the antibiotics albomycin and salmycin determined
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
ABC transporter system characterized, albomycin and salmycin sensitivity assays described, ferrichrome and ferrioxamine B transport assays applied, active transport of albomycin by the ABC transporter shown to mediate the high antibiotic efficacy of Streptococcus pneumoniae
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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ExbB; ExbD; FhuB, integral; TonB
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Manually annotated by BRENDA team
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FhuC membrane associated, preferential in the presence of FhuB, detected by FhuC antibodies
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Manually annotated by BRENDA team
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FhuB, integral; FhuC
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Manually annotated by BRENDA team
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fhuC, membrane bound; FhuD, membrane bound
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Manually annotated by BRENDA team
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FhuB, integral; FhuC, associated with the inside of the cytoplasmic membrane
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Manually annotated by BRENDA team
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FepC peripheral membrane protein
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Manually annotated by BRENDA team
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FepA, receptor, in conjunction with TonB and ExbB
Manually annotated by BRENDA team
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FhuD, periplasmic binding
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Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Staphylococcus aureus (strain COL)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25000 - 30000
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FhuD, SDS-PAGE
210337
28000
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FhuD, cloned, overexpressed, SDS-PAGE
210336
28190
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FhuE, nucleotide sequence
210341
28280
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FhuD, nucleotide sequence
210337
29260
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FhuC, nucleotide sequence
210337
29500
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FhuC, nucleotide sequence
210339
29500
-
FecD, SDS-PAGE
210341
30000
-
FhuC cloned, overexpressed, SDS-PAGE
210336
30000
-
FecB, SDS-PAGE, nucleotide sequence
210341
31000
-
FhuD, cloned, overexpressed, SDS-PAGE
210336
32000
-
FecC, SDS-PAGE
210341
34150
-
FecD
210341
35370
-
FecC, nucleotide sequence
210341
36000
-
FhuB, nucleotide sequence
210339
36100
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FhuG, nucleotide sequence
210339
45000
-
FhuB, unheated samples, SDS-PAGE
210336
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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ATP-transporter; components involved are FhuA (receptor), TonB, ExbB, ExbD and FhuC, FhuD, FhuB
additional information
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ATP-transporter; components involved are FhuA (receptor), TonB, ExbB, ExbD and FhuC, FhuD, FhuB; FhuB protein composed of two identical halfes: FhuB(N) amino acid residues 1-333 and FhuB(C)amino acid residues 334-660, active when combined in a single polypeptide chain
additional information
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ATP-transporter
additional information
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FhuD sites are asymmetrically arranged
additional information
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components involved are FhuC, FhuB, FhuG
additional information
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ATP-transporter; components involved in enterobactin uptake are FepA, TonB, ExbB, FepB, FepD, FepC, FepG; fhu-transport system for uptake of hydroxamates
additional information
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ATP-transporter; fec-transport system for citrate dependent iron uptake, components involved are FecA, FecB, FecC, FecD, FecE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FhuD protein, gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
fepD, fepG, fepC
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fhu cassette operon expressed in a chromosomal fhu deletion mutant
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fhuB,C,D cloned downstream of a phage T7 promoter, transcribed by t7 RNA polymerase, overexpression
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fhuC, FhuC fhuB, FhuA fhuC, fhuACDB cloned and overexpressed
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fhuC, fhuD
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fhuC, fhuB, fhuG
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fhuD derivative, expressed in Escherichia coli DH5-alpha and BL21-DE3 and SIP401, different plasmids used
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D172E
-
fhuC
D172N
-
fhuC
G226A
-
FhuB(N), negative effect on FhuB-mediated transport
G226E
-
FhuB(N), negative effect on FhuB-mediated transport
G226V
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FhuB(N), negative effect on FhuB-mediated transport
G559A
-
FhuB(C), negative effect on FhuB-mediated transport
G559E
-
FhuB(C), negative effect on FhuB-mediated transport
G559E
-
does not affect FhuD binding
G559V
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FhuB(C), negative effect on FhuB-mediated transport
K50E
-
fhuC
K50Q
-
fhuC
additional information
-
coupling of the reporter domain to different sites of FhuB(C); deletion in and of FhuB(N)
additional information
-
fhuC, deletion from Ile180 to Asp207, no phenotypic suppression; fhuC, duplication from Gly23 to Asp177, no phenotypic suppression
additional information
-
insertion duplication mutants and deletion mutants generated in the fhuD, fhuB, and fhuG genes, complementation studies performed, mutants in the fhuD, fhuB and fhuG genes shown to be transport inactive and resistant to the antibiotics albomycin and salmycin
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pharmacology
-
analysis of uptake of the antibiotic albomycin into cells of Streptococcus pneumoniae