Information on EC 3.6.3.34 - iron-chelate-transporting ATPase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
3.6.3.34
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RECOMMENDED NAME
GeneOntology No.
iron-chelate-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + iron chelate/out = ADP + phosphate + iron chelate/in
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
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transmembrane transport
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-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (iron-chelate-importing)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports Fe-enterobactin, Fe-dicitrate, Fe-hydroxamate and other siderophores.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
R6 strain
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + albomycin/out
ADP + phosphate + albomycin/in
show the reaction diagram
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-
-
-
?
ATP + H2O + desferrioxamine/out
ADP + phosphate + desferrioxamine/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + enterobactin/out
ADP + phosphate + enterobactin/in
show the reaction diagram
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fep-transport system
-
-
?
ATP + H2O + ferric dicitrate/out
ADP + phosphate + ferric dicitrate/in
show the reaction diagram
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fec-transport system
-
-
?
ATP + H2O + ferrichrome/out
ADP + phosphate + ferrichrome/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + ferrioxamine B/out
ADP + phosphate + ferrioxamine B/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
ATP + H2O + rhodotorulic acid/out
ADP + phosphate + rhodotorulic acid/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + salmycin/out
ADP + phosphate + salmycin/in
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + desferrioxamine/out
ADP + phosphate + desferrioxamine/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + enterobactin/out
ADP + phosphate + enterobactin/in
show the reaction diagram
-
fep-transport system
-
-
?
ATP + H2O + ferric dicitrate/out
ADP + phosphate + ferric dicitrate/in
show the reaction diagram
-
fec-transport system
-
-
?
ATP + H2O + ferrichrome/out
ADP + phosphate + ferrichrome/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + ferrioxamine B/out
ADP + phosphate + ferrioxamine B/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + iron aerobactin/out
ADP + phosphate + iron aerobactin/in
show the reaction diagram
ATP + H2O + iron coprogen/out
ADP + phosphate + iron coprogen/in
show the reaction diagram
-
-
-
-
?
ATP + H2O + iron ferrichrome/out
ADP + phosphate + iron ferrichrome/in
show the reaction diagram
ATP + H2O + iron(III) hydroxamates/out
ADP + phosphate + iron(III) hydroxamates/in
show the reaction diagram
ATP + H2O + rhodotorulic acid/out
ADP + phosphate + rhodotorulic acid/in
show the reaction diagram
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-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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cross-inhibition of ferric hydroxamates to the antibiotics albomycin and salmycin determined
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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ABC transporter system characterized, albomycin and salmycin sensitivity assays described, ferrichrome and ferrioxamine B transport assays applied, active transport of albomycin by the ABC transporter shown to mediate the high antibiotic efficacy of Streptococcus pneumoniae
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Staphylococcus aureus (strain COL)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000 - 30000
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FhuD, SDS-PAGE
28000
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FhuD, cloned, overexpressed, SDS-PAGE
28190
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FhuE, nucleotide sequence
28280
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FhuD, nucleotide sequence
29260
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FhuC, nucleotide sequence
31000
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FhuD, cloned, overexpressed, SDS-PAGE
32000
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FecC, SDS-PAGE
34150
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FecD
35370
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FecC, nucleotide sequence
36000
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FhuB, nucleotide sequence
36100
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FhuG, nucleotide sequence
45000
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FhuB, unheated samples, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FhuD protein, gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
fepD, fepG, fepC
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fhu cassette operon expressed in a chromosomal fhu deletion mutant
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fhuB,C,D cloned downstream of a phage T7 promoter, transcribed by t7 RNA polymerase, overexpression
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fhuC, fhuB, fhuG
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fhuC, FhuC fhuB, FhuA fhuC, fhuACDB cloned and overexpressed
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fhuC, fhuD
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fhuD derivative, expressed in Escherichia coli DH5-alpha and BL21-DE3 and SIP401, different plasmids used
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D172E
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fhuC
D172N
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fhuC
G226A
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FhuB(N), negative effect on FhuB-mediated transport
G226E
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FhuB(N), negative effect on FhuB-mediated transport
G226V
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FhuB(N), negative effect on FhuB-mediated transport
G559A
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FhuB(C), negative effect on FhuB-mediated transport
G559V
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FhuB(C), negative effect on FhuB-mediated transport
K50E
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fhuC
K50Q
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fhuC
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
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analysis of uptake of the antibiotic albomycin into cells of Streptococcus pneumoniae
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