Information on EC 3.6.3.31 - polyamine-transporting ATPase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
3.6.3.31
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RECOMMENDED NAME
GeneOntology No.
polyamine-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + polyamine/out = ADP + phosphate + polyamine/in
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
transmembrane transport
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (polyamine-importing)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports putrescine and spermidine.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + putrescine/out
ADP + phosphate + putrescine/in
show the reaction diagram
ATP + H2O + spermidine/out
ADP + phosphate + spermidine/in
show the reaction diagram
putrescine/out + ATP + H2O
putrescine/in + ADP + phosphate
show the reaction diagram
spermidine/out + ADP + H2O
spermidine/in + ?
show the reaction diagram
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the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP
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?
spermidine/out + ATP + H2O
spermidine/in + ADP + phosphate
show the reaction diagram
spermidine/out + CTP + H2O
spermidine/in + CDP + phosphate
show the reaction diagram
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the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP
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?
spermidine/out + GTP + H2O
spermidine/in + GDP + phosphate
show the reaction diagram
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the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP
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?
spermidine/out + UTP + H2O
spermidine/in + UDP + phosphate
show the reaction diagram
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the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP
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?
spermine/out + ATP + H2O
spermine/in + ADP + phosphate
show the reaction diagram
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two polyamine-uptake systems: one is a putrescine-specific system and the other is a spermidine-preferential system
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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Pot D is the primary receptor of the polyamine transport system, which regulates the polyamine content in E. coli
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NEM
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inhibits ATPase activity of Pot A
PCMB
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inhibits ATPase activity of Pot A
spermidine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 7
ATP
0.0015 - 0.005
putrescine/out
0.001
spermidine/out
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spermidine-preferential system
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.4
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ATPase activity of the potA subunit in the spermidine-preferential system
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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PotB and PotC contain six putative transmembrane segments linked by hydrophobic segments of variable length
Manually annotated by BRENDA team
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PotD is a periplasmic binding protein
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
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? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence
31000
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? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence; ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence
35000
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? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence
38000
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? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence
39000
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? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence
43000
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? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence
45000
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? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligomer
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? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence; ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization of the potD subunit and a potF - putrescine complex
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PotD protein complexed with spermidine
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PotF-putrescine complex
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
PotA protein
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PotD protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C26A
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no loss of ATPase activity
C276A
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no loss of ATPase activity
C54T
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mutated PotA protein loses both ATPase and spermidine uptake activity
D172N
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potA subunit, inactive mutant
E297D
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potA subunit, spermidine uptake activity is greately reduced
E297K
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potA subunit, spermidine uptake activity is reduced
E297Q
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potA subunit, spermidine uptake activity is greately reduced
F27L
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potA subunit, spermidine uptake activity is reduced
F47L
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potA subunit, spermidine uptake activity is reduced
L60F
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potA subunit, spermidine uptake activity is reduced
L76P
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spermidine uptake activity is reduced
additional information
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construction of mutants truncated at the carboxylic terminal domain of the potA subunit, these mutants have reduced activity
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