Information on EC 3.6.3.22 - nonpolar-amino-acid-transporting ATPase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.6.3.22
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RECOMMENDED NAME
GeneOntology No.
nonpolar-amino-acid-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + nonpolar amino acid/out = ADP + phosphate + nonpolar amino acid/in
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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transmembrane transport
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (nonpolar-amino-acid-transporting)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. Comprises bacterial enzymes that import Leu, Ile and Val.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PCC7120
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Manually annotated by BRENDA team
gene aap1or At1g58360
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
clinical isolate, live genes encoded in the liv operon
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Manually annotated by BRENDA team
clinical isolate, live genes encoded in the liv operon
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Manually annotated by BRENDA team
New Zealand White male rabbits
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Manually annotated by BRENDA team
strain Wisconsin 54-1255
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Manually annotated by BRENDA team
strain Wisconsin 54-1255
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + alanine/out
ADP + phosphate + alanine/in
show the reaction diagram
ATP + H2O + arginine/out
ADP + phosphate + arginine/in
show the reaction diagram
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-
-
-
?
ATP + H2O + aspartate/out
ADP + phosphate + aspartate/in
show the reaction diagram
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low activity
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?
ATP + H2O + glutamate/out
ADP + phosphate + glutamate/in
show the reaction diagram
ATP + H2O + glutamine/out
ADP + phosphate + glutamine/in
show the reaction diagram
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-
-
-
?
ATP + H2O + glycine/out
ADP + phosphate + glycine/in
show the reaction diagram
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high activity
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-
?
ATP + H2O + histidine/out
ADP + phosphate + histidine/in
show the reaction diagram
ATP + H2O + L-isoleucine/out
ADP + phosphate + L-isoleucine/in
show the reaction diagram
ATP + H2O + L-leucine/out
ADP + phosphate + L-leucine/in
show the reaction diagram
ATP + H2O + L-valine/out
ADP + phosphate + L-valine/in
show the reaction diagram
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45fold less transport compared to leucine
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?
ATP + H2O + leucine/out
ADP + phosphate + leucine/in
show the reaction diagram
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?
ATP + H2O + lysine/out
ADP + phosphate + lysine/in
show the reaction diagram
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-
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?
ATP + H2O + nonpolar amino acid/out
ADP + phosphate + nonpolar amino acid/in
show the reaction diagram
ATP + H2O + phenylalanine/out
ADP + phosphate + phenylalanine/in
show the reaction diagram
ATP + H2O + proline/out
ADP + phosphate + proline/in
show the reaction diagram
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-
-
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?
ATP + H2O + serine/out
ADP + phosphate + serine/in
show the reaction diagram
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best substrate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + alanine/out
ADP + phosphate + alanine/in
show the reaction diagram
ATP + H2O + arginine/out
ADP + phosphate + arginine/in
show the reaction diagram
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-
-
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?
ATP + H2O + glutamate/out
ADP + phosphate + glutamate/in
show the reaction diagram
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-
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?
ATP + H2O + glutamine/out
ADP + phosphate + glutamine/in
show the reaction diagram
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-
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?
ATP + H2O + glycine/out
ADP + phosphate + glycine/in
show the reaction diagram
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high activity
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?
ATP + H2O + histidine/out
ADP + phosphate + histidine/in
show the reaction diagram
ATP + H2O + L-leucine/out
ADP + phosphate + L-leucine/in
show the reaction diagram
ATP + H2O + leucine/out
ADP + phosphate + leucine/in
show the reaction diagram
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-
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?
ATP + H2O + lysine/out
ADP + phosphate + lysine/in
show the reaction diagram
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-
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?
ATP + H2O + nonpolar amino acid/out
ADP + phosphate + nonpolar amino acid/in
show the reaction diagram
ATP + H2O + phenylalanine/out
ADP + phosphate + phenylalanine/in
show the reaction diagram
ATP + H2O + proline/out
ADP + phosphate + proline/in
show the reaction diagram
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-
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?
ATP + H2O + serine/out
ADP + phosphate + serine/in
show the reaction diagram
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best substrate
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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leucine does not inhibit alpha-aminoadipic acid transport via PcDIP5
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methylprednisolone
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stimulates Na-alanine cotransport in the normal intestine, mechanism of stimulation is secondary to an increase in cotransporter numbers without an alteration in the affinity for the amino acid. Treatment with MP reverses the reduction in Na-alanine cotransport in villus cells from the chronically inflamed intestine, secondary to restoration in the affinity for the amino acid without a change in the Vmax, overview
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
alpha-aminoadipic acid
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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of roots and root hairs
Manually annotated by BRENDA team
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in cotyledons only
Manually annotated by BRENDA team
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from normal and chronically inflamed rabbits
Manually annotated by BRENDA team
additional information
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cellular and subcellular location of the Bgt and N-II transporters, N-I that is expressed only in vegetative cells, while the Bgt and N-II systems are present in both vegetative cells and heterocysts, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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cellular and subcellular location of the Bgt and N-II transporters, in nitrate-grown filaments, the recombinant GFP-tagged proteins are observed in all the cells of the filament, with a somewhat higher signal in the periphery than in the centre of the cells and, especially, in the intercellular septa, overview
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Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae mutant M4276
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ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA. Structure of the strain PCC 7120 genomic regions carrying the bgtB gene and the natF-natG-natH-bgtA gene cluster, overview, expression of the Bgt and N-II transporters as GFP-tagged proteins in strain PCC 7120
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
livJ expression is not induced by leucine
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information