Information on EC 3.6.3.20 - glycerol-3-phosphate-transporting ATPase

Word Map on EC 3.6.3.20
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.6.3.20
-
RECOMMENDED NAME
GeneOntology No.
glycerol-3-phosphate-transporting ATPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + glycerol-3-phosphate/out = ADP + phosphate + glycerol-3-phosphate/in
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transmembrane transport
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATPphosphohydrolase (glycerol-3-phosphate-importing)
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports phosphorylated glycerol.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
-
fosfomycin is widely used to treat urinary tract and pediatric gastrointestinal infections of bacteria. It is supposed that this antibiotic enters cells via two transport systems, including the bacterial glycerol-3-phosphate transporter, GlpT. Impaired function of GlpT is one mechanism for fosfomycin resistance. Interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + glycerol-3-phosphate/out
ADP + phosphate + glycerol-3-phosphate/in
show the reaction diagram
ATP + H2O + glycerol-3-phosphocholine/out
ADP + phosphate + glycerol-3-phosphocholine/in
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + glycerol-3-phosphate/out
ADP + phosphate + glycerol-3-phosphate/in
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
-
40.5% inhibition at 0.1 mM
bathophenanthroline
-
96.2% inhibition at 10 mM
fosfomycin
-
fosfomycin, externally added to the proteoliposomes, poorly inhibits the glycerol-3-phosphate/glycerol-3-phosphate antiport catalyzed by the reconstituted transporter, completely competitive, interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview
Mersalyl
-
71.1% inhibition at 0.1 mM
N-ethylmaleimide
-
1.5% inhibition at 1 mM
Phthalic acid
-
48.8% inhibition at 10 mM
pyridoxal 5'-phosphate
-
98.5% inhibition at 10 mM
additional information
not inhibited by phosphate or by the signal transducing protein PhoU or the phosphodiesterase UgpQ
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
kinetics, rocker-switch mechanism of membrane transport by GlpT, overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.4
fosfomycin
Escherichia coli
-
externally added to the proteoliposomes with reconstituted transporter, pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
substrate binding affinity assays
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by vapor diffusion, to 3.3 A resolution
-
in complex with glycerol-3-phosphate, sitting drop vapor diffusion method, using 5 mM CoCl3, 5 mM NiCl2, 5 mM CdCl2, 5 mM MgCl2, 0.1 M HEPES, pH 7.5, 12% (w/v) polyethylene glycol 3350, and 0.3% octyl-beta-D-glucopyranoside
X-ray structure, GlpT consists of 12 transmembrane helices, which form a N- and C-terminal domain, the structure reveals a large internal cavity, open toward the cytoplasm but completely closed to the periplasm, the substrate-binding site, formed by two arginine residues, is accessible from only one side of the membrane at a time
-
crystal structure analysis of GlpT for structure modelling
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by Ni-NTA affinity column and size-exclusion chromatography
-
Ni-NTA agarose column chromatography
recombinant His-tagged enzyme 55fold from Escherichia coli strain SH116 by nickel affinity chromatography
-
recombinant His-tagged wild-type enzyme from mutant GlpT-deficient strain SH1200 by ultracentrifugation and nickel affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 T1 DE3 cells
expression of His-tagged enzyme in Escherichia coli strain SH116
-
gene ArG3Pp3, cloning of 5 genes encoding G3Pps, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis. Differential expression patterns of the promoter-GUS fusion transgenics for the G3Pp gene family during seedling growth, overview; gene AtG3Pp1, cloning of 5 genes encoding G3Pps, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis. Differential expression patterns of the promoter-GUS fusion transgenics for the G3Pp gene family during seedling growth, overview; gene AtG3Pp2, cloning of 5 genes encoding G3Pps, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis. Differential expression patterns of the promoter-GUS fusion transgenics for the G3Pp gene family during seedling growth, overview; gene AtG3Pp4, cloning of 5 genes encoding G3Pps, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis. Differential expression patterns of the promoter-GUS fusion transgenics for the G3Pp gene family during seedling growth, overview; gene AtG3Pp5, cloning of 5 genes encoding G3Pps, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis. Differential expression patterns of the promoter-GUS fusion transgenics for the G3Pp gene family during seedling growth, overview
into vector pBAD-MycHis-A and expressed in LMG194 cells
-
overexpression of His-tagged wild-type enzyme in mutant GlpT-deficient strain SH1200
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene AtG3Pp1 is induced 24fold in the roots of phosphate-deprived seedlings; gene AtG3Pp2 is induced 3fold in the roots of phosphate-deprived seedlings; phosphate deficiency-mediated induction of AtG3Pp4 is evident in both roots and shoots; phosphate deficiency-mediated induction of gene AtG3Pp3 is evident in both roots and shoots
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G149E
-
site-directed mutagenesis, structure analysis
R28C
-
site-directed mutagenesis, structure analysis
R28C/W118R/G149E
-
site-directed mutagenesis, structure analysis
W118R
-
site-directed mutagenesis, structure analysis
additional information
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, reconstitution of GlpT into proteoliposomes, overview
-
reconstitution of the recombinant His-tagged GlpT into liposomes
-
Show AA Sequence (3974 entries)
Please use the Sequence Search for a specific query.